{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T02:41:36Z","timestamp":1773283296051,"version":"3.50.1"},"reference-count":40,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2007,9,1]],"date-time":"2007-09-01T00:00:00Z","timestamp":1188604800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2007,9]]},"abstract":"ABSTRACT<\/jats:title>\n \n The aim of this study was to analyze the distributions of antibiotic susceptibility patterns, serotypes, phenotypes, genotypes, and macrolide resistance genes among 125 nonduplicated erythromycin-resistant\n Streptococcus pneumoniae<\/jats:italic>\n clinical isolates collected in a Spanish point prevalence study. The prevalence of resistance to macrolides in this study was 34.7%. Multiresistance (to three or more antimicrobials) was observed in 81.6% of these strains. Among 15 antimicrobials studied, cefotaxime, moxifloxacin, telithromycin, and quinupristin-dalfopristin were the most active drugs. The most frequent serotypes of erythromycin-resistant isolates were 19F (25%), 19A (17%), 6B (12%), 14 (10%), and 23F (10%). Of the 125 strains, 109 (87.2%) showed the MLS\n B<\/jats:sub>\n phenotype [103 had the\n erm<\/jats:italic>\n (B) gene and 6 had both\n erm<\/jats:italic>\n (B) and\n mef<\/jats:italic>\n (E) genes]. Sixteen (12.8%) strains showed the M phenotype [14 with\n mef<\/jats:italic>\n (E) and 2 with\n mef<\/jats:italic>\n (A)]. All isolates were tested by PCR for the presence of the\n int, xis, tnpR<\/jats:italic>\n , and\n tnpA<\/jats:italic>\n genes associated with conjugative transposons (Tn\n 916<\/jats:italic>\n family and Tn\n 917<\/jats:italic>\n ). Positive detection of\n erm<\/jats:italic>\n (B),\n tet<\/jats:italic>\n (M),\n int<\/jats:italic>\n , and\n xis<\/jats:italic>\n genes related to the Tn\n 916<\/jats:italic>\n family was found in 77.1% of MLS\n B<\/jats:sub>\n phenotype strains. In 16 strains, only the\n tndX, erm<\/jats:italic>\n (B), and\n tet<\/jats:italic>\n (M) genes were detected, suggesting the presence of Tn\n 1116<\/jats:italic>\n , a transposon recently described for\n Streptococcus pyogenes<\/jats:italic>\n . Five clones, namely, Sweden\n 15A<\/jats:sup>\n -25, clone\n 19F<\/jats:sup>\n ST87, Spain\n 23F<\/jats:sup>\n -1, Spain\n 6B<\/jats:sup>\n -2, and clone\n 19A<\/jats:sup>\n ST276, accounted for half of the MLS\n B<\/jats:sub>\n strains. In conclusion, the majority of erythromycin-resistant pneumococci isolated in Spain had the MLS\n B<\/jats:sub>\n phenotype, belonged to multiresistant international clones, and carried the\n erm<\/jats:italic>\n (B),\n tet<\/jats:italic>\n (M),\n xis<\/jats:italic>\n , and\n int<\/jats:italic>\n genes, suggesting the spread of transposons of the Tn\n 916<\/jats:italic>\n family.\n <\/jats:p>","DOI":"10.1128\/aac.00157-07","type":"journal-article","created":{"date-parts":[[2007,7,3]],"date-time":"2007-07-03T00:39:36Z","timestamp":1183423176000},"page":"3240-3246","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":45,"title":["Serotypes, Clones, and Mechanisms of Resistance of Erythromycin-Resistant\n Streptococcus pneumoniae<\/i>\n Isolates Collected in Spain"],"prefix":"10.1128","volume":"51","author":[{"given":"Laura","family":"Calatayud","sequence":"first","affiliation":[{"name":"Microbiology Department, Hospital Universitari de Bellvitge, University of Barcelona, IDIBELL, Barcelona, Spain"},{"name":"Ciber de Enfermedades Respiratorias, ISCIII, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Ardanuy","sequence":"additional","affiliation":[{"name":"Microbiology Department, Hospital Universitari de Bellvitge, University of Barcelona, IDIBELL, Barcelona, Spain"},{"name":"Ciber de Enfermedades Respiratorias, ISCIII, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Cercenado","sequence":"additional","affiliation":[{"name":"Ciber de Enfermedades Respiratorias, ISCIII, Madrid, Spain"},{"name":"Microbiology Department, Hospital Gregorio Maran\u0303o\u0301n, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Fenoll","sequence":"additional","affiliation":[{"name":"Microbiology Department, Centro Nacional de Microbiologi\u0301a, Instituto de Salud Carlos III, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Bouza","sequence":"additional","affiliation":[{"name":"Ciber de Enfermedades Respiratorias, ISCIII, Madrid, Spain"},{"name":"Microbiology Department, Hospital Gregorio Maran\u0303o\u0301n, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Pallares","sequence":"additional","affiliation":[{"name":"Ciber de Enfermedades Respiratorias, ISCIII, Madrid, Spain"},{"name":"Infectious Diseases Department, Clinical Research Unit, Hospital Universitari de Bellvitge, University of Barcelona, IDIBELL, Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Marti\u0301n","sequence":"additional","affiliation":[{"name":"Microbiology Department, Hospital Universitari de Bellvitge, University of Barcelona, IDIBELL, Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Lin\u0303ares","sequence":"additional","affiliation":[{"name":"Microbiology Department, Hospital Universitari de Bellvitge, University of Barcelona, IDIBELL, Barcelona, Spain"},{"name":"Ciber de Enfermedades Respiratorias, ISCIII, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.9.3313-3318.2002"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.2.827-829.2005"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00269-06"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2006.9997"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01484-06"},{"key":"e_1_3_2_7_2","unstructured":"Abstr. 16th Eur. 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