{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T11:27:58Z","timestamp":1790681278671,"version":"4.1.0"},"reference-count":39,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2015,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Three\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Klebsiella pneumoniae<\/jats:named-content>\n            clinical isolates demonstrating carbapenem resistance were recovered from different patients hospitalized at two medical centers in S\u00e3o Paulo, Brazil. Resistance to all \u03b2-lactams, quinolones, and some aminoglycosides was observed for these isolates that were susceptible to polymyxin B. Carbapenem hydrolysis, which was inhibited by clavulanic acid, was observed for all\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">K. pneumoniae<\/jats:named-content>\n            isolates that belonged to the same pulsed-field gel electrophoresis (PFGE) type and a novel sequence type (ST), ST1781 (clonal complex 442 [CC442]). A 10-kb nonconjugative incompatibility group Q (IncQ) plasmid, denominated p60136, was transferred to\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Escherichia coli<\/jats:named-content>\n            strain TOP10 cells by electroporation. The full sequencing of p60136 showed that it was composed of a mobilization system, IS\n            <jats:italic>Kpn23<\/jats:italic>\n            , the phosphotransferase aph3A-VI, and a 941-bp open reading frame (ORF) that codified a 313-amino acid protein. This ORF was named\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>BKC-1<\/jats:sub>\n            . Brazilian\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Klebsiella<\/jats:named-content>\n            carbapenemase-1 (BKC-1) showed a pI of 6.0 and possessed the highest identity (63%) with a \u03b2-lactamase of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Sinorhizobium meliloti<\/jats:named-content>\n            , an environmental bacterium. Hydrolysis studies demonstrated that purified BKC-1 not only hydrolyzed carbapenems but also penicillins, cephalosporins, and monobactams. However, the carbapenems were less efficiently hydrolyzed due to their very low\n            <jats:italic>\n              k\n              <jats:sub>cat<\/jats:sub>\n            <\/jats:italic>\n            values (0.0016 to 0.031 s\n            <jats:sup>\u22121<\/jats:sup>\n            ). In fact, oxacillin was the best substrate for BKC-1 (\n            <jats:italic>\n              k\n              <jats:sub>cat<\/jats:sub>\n            <\/jats:italic>\n            \/\n            <jats:italic>\n              K\n              <jats:sub>m<\/jats:sub>\n            <\/jats:italic>\n            , 53,522.6 mM\n            <jats:sup>\u22121<\/jats:sup>\n            s\n            <jats:sup>\u22121<\/jats:sup>\n            ). Here, we report a new class A carbapenemase, confirming the diversity and rapid evolution of \u03b2-lactamases in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">K. pneumoniae<\/jats:named-content>\n            clinical isolates.\n          <\/jats:p>","DOI":"10.1128\/aac.00158-15","type":"journal-article","created":{"date-parts":[[2015,6,10]],"date-time":"2015-06-10T19:10:15Z","timestamp":1433963415000},"page":"5159-5164","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":78,"title":["Characterization of BKC-1 Class A Carbapenemase from Klebsiella pneumoniae Clinical Isolates in Brazil"],"prefix":"10.1128","volume":"59","author":[{"given":"Adriana Giannini","family":"Nicoletti","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio Alerta, Disciplina de Infectologia, Departamento de Medicina, Universidade Federal de S\u00e3o Paulo, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcelo F. M.","family":"Marcondes","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Enzimologia, Departamento de Biof\u00edsica, Universidade Federal de S\u00e3o Paulo, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Willames M. B. S.","family":"Martins","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio Alerta, Disciplina de Infectologia, Departamento de Medicina, Universidade Federal de S\u00e3o Paulo, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiz G. 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