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We prospectively collected 80 positive blood cultures (Bactec-FX system, Becton-Dickinson, Cockeysville, MD, USA) with echinocandin-susceptible\n            <jats:named-content content-type=\"genus-species\">Candida<\/jats:named-content>\n            spp. (\n            <jats:italic>n<\/jats:italic>\n            = 60) and echinocandin-intermediate\n            <jats:named-content content-type=\"genus-species\">Candida parapsilosis<\/jats:named-content>\n            (\n            <jats:italic>n<\/jats:italic>\n            = 20) from patients with candidemia. Additionally, blood culture bottles of nonfungemic\/bacteremic patients were spiked with 35 echinocandin-resistant\n            <jats:named-content content-type=\"genus-species\">Candida<\/jats:named-content>\n            species isolates. A total of 2 to 4 drops of medium from each bottle were stroked directly onto both RPMI 1640 agar plates with micafungin and anidulafungin Etest strips (ET\n            <jats:sub>DIR<\/jats:sub>\n            ) and Sabouraud agar plates containing 2 mg\/liter of anidulafungin. The isolates were tested according to the EUCAST method and Etest standard (ET\n            <jats:sub>SD<\/jats:sub>\n            ). Essential and categorical agreement between the methods was calculated. The essential agreement and categorical agreement between the EUCAST method and ET\n            <jats:sub>DIR<\/jats:sub>\n            and ET\n            <jats:sub>SD<\/jats:sub>\n            were both &gt;97.4%. The essential agreement between ET\n            <jats:sub>DIR<\/jats:sub>\n            and the EUCAST method for both echinocandins was &gt;97%. The categorical agreement between the\n            <jats:italic>FKS<\/jats:italic>\n            sequence and ET\n            <jats:sub>DIR<\/jats:sub>\n            was 97.4%. The ET\n            <jats:sub>DIR<\/jats:sub>\n            MICs of anidulafungin and micafungin (\u22650.19 mg\/liter and \u22650.064 mg\/liter, respectively) effectively separated all susceptible\n            <jats:italic>FKS<\/jats:italic>\n            wild-type isolates from the resistant\n            <jats:italic>FKS<\/jats:italic>\n            mutant isolates. The categorical agreement (62.6%) between the EUCAST method and growth on anidulafungin-containing plates was poor, with the best agreement observed for\n            <jats:named-content content-type=\"genus-species\">Candida glabrata<\/jats:named-content>\n            (94.2%). When performed directly on positive blood cultures from patients with candidemia, the Etest with micafungin and anidulafungin is a reliable procedure for the rapid testing of susceptibility to echinocandins in\n            <jats:named-content content-type=\"genus-species\">Candida<\/jats:named-content>\n            species isolates.\n          <\/jats:p>","DOI":"10.1128\/aac.00162-18","type":"journal-article","created":{"date-parts":[[2018,4,25]],"date-time":"2018-04-25T10:44:37Z","timestamp":1524653077000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":10,"title":["Resistance to Echinocandins in Candida Can Be Detected by Performing the Etest Directly on Blood Culture Samples"],"prefix":"10.1128","volume":"62","author":[{"given":"Mar\u00eda \u00c1ngeles","family":"Bordallo-Cardona","sequence":"first","affiliation":[{"name":"Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Mara\u00f1\u00f3n, Madrid, Spain"},{"name":"Instituto de Investigaci\u00f3n Sanitaria Gregorio Mara\u00f1\u00f3n, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura Judith","family":"Marcos-Zambrano","sequence":"additional","affiliation":[{"name":"Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Mara\u00f1\u00f3n, Madrid, Spain"},{"name":"Instituto de Investigaci\u00f3n Sanitaria Gregorio Mara\u00f1\u00f3n, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos","family":"S\u00e1nchez-Carrillo","sequence":"additional","affiliation":[{"name":"Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Mara\u00f1\u00f3n, Madrid, Spain"},{"name":"Instituto de Investigaci\u00f3n Sanitaria Gregorio Mara\u00f1\u00f3n, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emilio","family":"Bouza","sequence":"additional","affiliation":[{"name":"Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Mara\u00f1\u00f3n, Madrid, Spain"},{"name":"Instituto de Investigaci\u00f3n Sanitaria Gregorio Mara\u00f1\u00f3n, Madrid, Spain"},{"name":"CIBER Enfermedades Respiratorias-CIBERES (CB06\/06\/0058), Madrid, Spain"},{"name":"Medicine Department, School of Medicine, Universidad Complutense de Madrid, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricia","family":"Mu\u00f1oz","sequence":"additional","affiliation":[{"name":"Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Mara\u00f1\u00f3n, Madrid, Spain"},{"name":"Instituto de Investigaci\u00f3n Sanitaria Gregorio Mara\u00f1\u00f3n, Madrid, Spain"},{"name":"CIBER Enfermedades Respiratorias-CIBERES (CB06\/06\/0058), Madrid, Spain"},{"name":"Medicine Department, School of Medicine, Universidad Complutense de Madrid, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pilar","family":"Escribano","sequence":"additional","affiliation":[{"name":"Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Mara\u00f1\u00f3n, Madrid, Spain"},{"name":"Instituto de Investigaci\u00f3n Sanitaria Gregorio Mara\u00f1\u00f3n, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7901-8355","authenticated-orcid":false,"given":"Jes\u00fas","family":"Guinea","sequence":"additional","affiliation":[{"name":"Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Mara\u00f1\u00f3n, Madrid, Spain"},{"name":"Instituto de Investigaci\u00f3n Sanitaria Gregorio Mara\u00f1\u00f3n, Madrid, Spain"},{"name":"CIBER Enfermedades Respiratorias-CIBERES (CB06\/06\/0058), Madrid, 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