{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T06:04:30Z","timestamp":1790834670041,"version":"4.1.0"},"reference-count":49,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2013,7,1]],"date-time":"2013-07-01T00:00:00Z","timestamp":1372636800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2013,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            This study describes three novel\n            <jats:italic>erm<\/jats:italic>\n            (T)-carrying multiresistance plasmids that also harbor cadmium and copper resistance determinants. The plasmids, designated pUR1902, pUR2940, and pUR2941, were obtained from porcine and human methicillin-resistant\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Staphylococcus aureus<\/jats:named-content>\n            (MRSA) of the clonal lineage ST398. In addition to the macrolide-lincosamide-streptogramin B (MLS\n            <jats:sub>B<\/jats:sub>\n            ) resistance gene\n            <jats:italic>erm<\/jats:italic>\n            (T), all three plasmids also carry the tetracycline resistance gene\n            <jats:italic>tet<\/jats:italic>\n            (L). Furthermore, plasmid pUR2940 harbors the trimethoprim resistance gene\n            <jats:italic>dfrK<\/jats:italic>\n            and the MLS\n            <jats:sub>B<\/jats:sub>\n            resistance gene\n            <jats:italic>erm<\/jats:italic>\n            (C), while plasmids pUR1902 and pUR2941 possess the kanamycin\/neomycin resistance gene\n            <jats:italic>aadD<\/jats:italic>\n            . Sequence analysis of approximately 18.1 kb of the\n            <jats:italic>erm<\/jats:italic>\n            (T)-flanking region from pUR1902, 20.0 kb from pUR2940, and 20.8 kb from pUR2941 revealed the presence of several copies of the recently described insertion sequence IS\n            <jats:italic>Sau10<\/jats:italic>\n            , which is probably involved in the evolution of the respective plasmids. All plasmids carried a functional cadmium resistance operon with the genes\n            <jats:italic>cadD<\/jats:italic>\n            and\n            <jats:italic>cadX<\/jats:italic>\n            , in addition to the multicopper oxidase gene\n            <jats:italic>mco<\/jats:italic>\n            and the ATPase copper transport gene\n            <jats:italic>copA<\/jats:italic>\n            , which are involved in copper resistance. The comparative analysis of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">S. aureus<\/jats:named-content>\n            RN4220 and the three\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">S. aureus<\/jats:named-content>\n            RN4220 transformants carrying plasmid pUR1902, pUR2940, or pUR2941 revealed an 8-fold increase in CdSO\n            <jats:sub>4<\/jats:sub>\n            and a 2-fold increase in CuSO\n            <jats:sub>4<\/jats:sub>\n            MICs. The emergence of multidrug resistance plasmids that also carry heavy metal resistance genes is alarming and requires further surveillance. The colocalization of antimicrobial resistance genes and genes that confer resistance to heavy metals may facilitate their persistence, coselection, and dissemination.\n          <\/jats:p>","DOI":"10.1128\/aac.00171-13","type":"journal-article","created":{"date-parts":[[2013,4,30]],"date-time":"2013-04-30T03:01:41Z","timestamp":1367290901000},"page":"3275-3282","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":85,"title":["Novel\n            <i>erm<\/i>\n            (T)-Carrying Multiresistance Plasmids from Porcine and Human Isolates of Methicillin-Resistant Staphylococcus aureus ST398 That Also Harbor Cadmium and Copper Resistance Determinants"],"prefix":"10.1128","volume":"57","author":[{"given":"Elena","family":"G\u00f3mez-Sanz","sequence":"first","affiliation":[{"name":"Biochemistry and Molecular Biology, University of La Rioja, Logro\u00f1o, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristina","family":"Kadlec","sequence":"additional","affiliation":[{"name":"Institute of Farm Animal Genetics, Friedrich-Loeffler-Institut, Neustadt-Mariensee, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea T.","family":"Fe\u00dfler","sequence":"additional","affiliation":[{"name":"Institute of Farm Animal Genetics, Friedrich-Loeffler-Institut, Neustadt-Mariensee, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Myriam","family":"Zarazaga","sequence":"additional","affiliation":[{"name":"Biochemistry and Molecular Biology, University of La Rioja, Logro\u00f1o, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen","family":"Torres","sequence":"additional","affiliation":[{"name":"Biochemistry and Molecular Biology, University of La Rioja, Logro\u00f1o, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Schwarz","sequence":"additional","affiliation":[{"name":"Institute of Farm Animal Genetics, Friedrich-Loeffler-Institut, Neustadt-Mariensee, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2012.03842.x"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02260-10"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkq021"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00561-11"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2010.0610"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkp350"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkp378"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkr497"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1006\/plas.1994.1007"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.12.3362-3365.2001"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01325-07"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01091-09"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02799-06"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cimid.2012.10.001"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkr348"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00027-12"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02398-12"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00305-11"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1804.111419"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1608.100317"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0028369"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dks411"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-010-0389-4"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2011.06275.x"},{"key":"e_1_3_2_26_2","first-page":"99","volume-title":"Antimicrobial resistance in bacteria of animal origin","author":"Hasman H","year":"2006","unstructured":"HasmanH FrankeS RensingC. 2006. 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