{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,25]],"date-time":"2025-04-25T11:07:30Z","timestamp":1745579250388},"reference-count":41,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2014,2,1]],"date-time":"2014-02-01T00:00:00Z","timestamp":1391212800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2014,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The bacterial fatty acid biosynthesis pathway is a validated target for the development of novel chemotherapeutics. However, since\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Burkholderia pseudomallei<\/jats:named-content>\n            carries genes that encode both FabI and FabV enoyl-acyl carrier protein (ACP) reductase homologues, the enoyl-ACP reductase that is essential for\n            <jats:italic>in vivo<\/jats:italic>\n            growth needs to be defined so that the correct drug target can be chosen for development. Accordingly, \u0394\n            <jats:italic>fabI1<\/jats:italic>\n            , \u0394\n            <jats:italic>fabI2<\/jats:italic>\n            , and \u0394\n            <jats:italic>fabV<\/jats:italic>\n            knockout strains were constructed and tested in a mouse model of infection. Mice infected with a \u0394\n            <jats:italic>fabI1<\/jats:italic>\n            strain did not show signs of morbidity, mortality, or dissemination after 30 days of infection compared to the wild-type and \u0394\n            <jats:italic>fabI2<\/jats:italic>\n            and \u0394\n            <jats:italic>fabV<\/jats:italic>\n            mutant strains that had times to mortality of 60 to 84 h. Although signs of morbidity and mortality of \u0394\n            <jats:italic>fabI2<\/jats:italic>\n            and \u0394\n            <jats:italic>fabV<\/jats:italic>\n            strains were not significantly different from those of the wild-type strain, a slight delay was observed. A FabI1-specific inhibitor was used to confirm that inhibition of FabI1 results in reduced bacterial burden and efficacy in an acute\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">B. pseudomallei<\/jats:named-content>\n            murine model of infection. This work establishes that FabI1 is required for growth of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Burkholderia pseudomallei<\/jats:named-content>\n            <jats:italic>in vivo<\/jats:italic>\n            and is a potential molecular target for drug development.\n          <\/jats:p>","DOI":"10.1128\/aac.00176-13","type":"journal-article","created":{"date-parts":[[2013,11,26]],"date-time":"2013-11-26T03:28:07Z","timestamp":1385436487000},"page":"931-935","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":15,"title":["The Burkholderia pseudomallei Enoyl-Acyl Carrier Protein Reductase FabI1 Is Essential for\n            <i>In Vivo<\/i>\n            Growth and Is the Target of a Novel Chemotherapeutic with Efficacy"],"prefix":"10.1128","volume":"58","author":[{"given":"Jason E.","family":"Cummings","sequence":"first","affiliation":[{"name":"Rocky Mountain Regional Center of Excellence for Biodefense and Emerging Infectious Diseases Research and Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luke C.","family":"Kingry","sequence":"additional","affiliation":[{"name":"Rocky Mountain Regional Center of Excellence for Biodefense and Emerging Infectious Diseases Research and Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Drew A.","family":"Rholl","sequence":"additional","affiliation":[{"name":"Rocky Mountain Regional Center of Excellence for Biodefense and Emerging Infectious Diseases Research and Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Herbert P.","family":"Schweizer","sequence":"additional","affiliation":[{"name":"Rocky Mountain Regional Center of Excellence for Biodefense and Emerging Infectious Diseases Research and Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter J.","family":"Tonge","sequence":"additional","affiliation":[{"name":"Institute for Chemical Biology &amp; Drug Discovery, Department of Chemistry, Stony Brook University, Stony Brook, New York, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard A.","family":"Slayden","sequence":"additional","affiliation":[{"name":"Rocky Mountain Regional Center of Excellence for Biodefense and Emerging Infectious Diseases Research and Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.18.2.383-416.2005"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1385"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2009.00189.x"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.qco.0000244046.31135.b3"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000900"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0035-9203(08)70002-6"},{"key":"e_1_3_2_8_2","first-page":"1438","article-title":"Melioidosis\u2013a report from Pondicherry, South India","volume":"50","author":"Kanungo R","year":"2002","unstructured":"KanungoRPadhanPBhattacharyaSSrimannarayanaJJayanthiSSwaminathanRP. 2002. 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