{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T12:22:09Z","timestamp":1790338929465,"version":"4.1.0"},"reference-count":32,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2016,8,1]],"date-time":"2016-08-01T00:00:00Z","timestamp":1470009600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2016,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>A simple and rapid ultra-high-performance liquid chromatography (UHPLC) method using UV detection was developed for the simultaneous determination of eight \u03b2-lactam antibiotics in human plasma, including four penicillins, amoxicillin (AMX), cloxacillin (CLX), oxacillin (OXA), and piperacillin (PIP), and four cephalosporins, cefazolin (CFZ), cefepime (FEP), cefotaxime (CTX), and ceftazidime (CAZ). One hundred-microliter samples were spiked with thiopental as an internal standard, and proteins were precipitated by acetonitrile containing 0.1% formic acid. Separation was achieved on a pentafluorophenyl (PFP) column with a mobile phase composed of phosphoric acid (10 mM) and acetonitrile in gradient elution mode at a flow rate of 500 \u03bcl\/min. Detection was performed at 230 nm for AMX, CLX, OXA, and PIP and 260 nm for CFZ, FEP, CTX, and CAZ. The total analysis time did not exceed 13 min. The method was found to be linear at concentrations ranging from 2 to 100 mg\/liter for each compound, and all validation parameters fulfilled international requirements. Between- and within-run accuracy errors ranged from \u22125.2% to 11.4%, and precision was lower than 14.2%. This simple method requires small-volume samples and can easily be implemented in most clinical laboratories to promote the therapeutic drug monitoring of \u03b2-lactam antibiotics. The simultaneous determination of several antibiotics considerably reduces the time to results for clinicians, which may improve treatment efficiency, especially in critically ill patients.<\/jats:p>","DOI":"10.1128\/aac.00176-16","type":"journal-article","created":{"date-parts":[[2016,5,24]],"date-time":"2016-05-24T16:26:28Z","timestamp":1464107188000},"page":"4734-4742","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":77,"title":["Simultaneous Determination of Eight \u03b2-Lactam Antibiotics, Amoxicillin, Cefazolin, Cefepime, Cefotaxime, Ceftazidime, Cloxacillin, Oxacillin, and Piperacillin, in Human Plasma by Using Ultra-High-Performance Liquid Chromatography with Ultraviolet Detection"],"prefix":"10.1128","volume":"60","author":[{"given":"Tiphaine","family":"Legrand","sequence":"first","affiliation":[{"name":"Laboratoire de Pharmacologie-Toxicologie, Assistance Publique des H\u00f4pitaux de Paris, H\u00f4pitaux Universitaires Henri Mondor, Cr\u00e9teil, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominique","family":"Vodovar","sequence":"additional","affiliation":[{"name":"Service d'Anesth\u00e9sie et des R\u00e9animations Chirurgicales, Assistance Publique des H\u00f4pitaux de Paris, H\u00f4pitaux Universitaires Henri Mondor, Cr\u00e9teil, France"},{"name":"IMIV, CEA, INSERM, CNRS, Universit\u00e9 Paris-Sud, Universit\u00e9 Paris Saclay, CEA-SHFJ, Orsay, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Tournier","sequence":"additional","affiliation":[{"name":"IMIV, CEA, INSERM, CNRS, Universit\u00e9 Paris-Sud, Universit\u00e9 Paris Saclay, CEA-SHFJ, Orsay, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nihel","family":"Khoudour","sequence":"additional","affiliation":[{"name":"Laboratoire de Pharmacologie-Toxicologie, Assistance Publique des H\u00f4pitaux de Paris, H\u00f4pitaux Universitaires Henri Mondor, Cr\u00e9teil, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Hulin","sequence":"additional","affiliation":[{"name":"Laboratoire de Pharmacologie-Toxicologie, Assistance Publique des H\u00f4pitaux de Paris, H\u00f4pitaux Universitaires Henri Mondor, Cr\u00e9teil, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2333"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1097\/FTD.0b013e3181e60ca6"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2010.06.008"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1186\/cc9091"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1186\/cc10441"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.4155\/bio.10.180"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkt240"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkv201"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201507-1487ED"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.5603\/AIT.a2015.0082"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-005-0021-y"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2125.2011.03991.x"},{"key":"e_1_3_1_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2012.10.002"},{"key":"e_1_3_1_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(14)70036-2"},{"key":"e_1_3_1_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2007.06.002"},{"key":"e_1_3_1_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2015.06.016"},{"key":"e_1_3_1_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2008.01.037"},{"key":"e_1_3_1_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2009.06.034"},{"key":"e_1_3_1_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2010.05.027"},{"key":"e_1_3_1_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00533-11"},{"key":"e_1_3_1_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2012.10.024"},{"key":"e_1_3_1_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2011.03.001"},{"key":"e_1_3_1_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2012.06.022"},{"key":"e_1_3_1_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2012.10.046"},{"key":"e_1_3_1_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2013.11.004"},{"key":"e_1_3_1_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2014.04.029"},{"key":"e_1_3_1_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2014.11.034"},{"key":"e_1_3_1_29_2","unstructured":"European Medicines Agency. 2011. 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