{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T02:38:46Z","timestamp":1773283126125,"version":"3.50.1"},"reference-count":32,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2018,6,1]],"date-time":"2018-06-01T00:00:00Z","timestamp":1527811200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001412","name":"Council of Scientific and Industrial Research, India","doi-asserted-by":"crossref","award":["F. No. 09\/174(0068)\/2014-EMR-I"],"award-info":[{"award-number":["F. No. 09\/174(0068)\/2014-EMR-I"]}],"id":[{"id":"10.13039\/501100001412","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2018,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:named-content content-type=\"genus-species\">Candida glabrata<\/jats:named-content>\n                    infections are increasing worldwide and exhibit greater rates of antifungal resistance than those with other species. DNA mismatch repair (MMR) gene deletions, such as\n                    <jats:italic>msh2\u0394<\/jats:italic>\n                    , in\n                    <jats:named-content content-type=\"genus-species\">C. glabrata<\/jats:named-content>\n                    resulting in a mutator phenotype have recently been reported to facilitate rapid acquisition of antifungal resistance. This study determined the antifungal susceptibility profiles of 210\n                    <jats:named-content content-type=\"genus-species\">C. glabrata<\/jats:named-content>\n                    isolates in 10 hospitals in India and investigated the impact of novel\n                    <jats:italic>MSH2<\/jats:italic>\n                    polymorphisms on mutation potential. No echinocandin- or azole-resistant strains and no mutations in\n                    <jats:italic>FKS<\/jats:italic>\n                    hot spot regions were detected among the\n                    <jats:named-content content-type=\"genus-species\">C. glabrata<\/jats:named-content>\n                    isolates, supporting our\n                    <jats:italic>in vitro<\/jats:italic>\n                    susceptibility testing results. CLSI antifungal susceptibility data showed that the MICs of anidulafungin (geometric mean [GM], 0.12 \u03bcg\/ml) and micafungin (GM, 0.01 \u03bcg\/ml) were lower and below the susceptibility breakpoint compared to that of caspofungin (CAS) (GM, 1.31 \u03bcg\/ml). Interestingly, 69% of the\n                    <jats:named-content content-type=\"genus-species\">C. glabrata<\/jats:named-content>\n                    strains sequenced contained six nonsynonymous mutations in\n                    <jats:italic>MSH2<\/jats:italic>\n                    , i.e., V239L and the novel mutations E459K, R847C, Q386K, T772S, and V239\/D946E. Functional analysis of\n                    <jats:italic>MSH2<\/jats:italic>\n                    mutations revealed that 49% of the tested strains (40\/81) contained a partial loss-of-function\n                    <jats:italic>MSH2<\/jats:italic>\n                    mutation. The novel\n                    <jats:italic>MSH2<\/jats:italic>\n                    substitution Q386K produced higher frequencies of CAS-resistant colonies upon expression in the\n                    <jats:italic>msh2\u0394<\/jats:italic>\n                    mutant. However, expression of two other novel\n                    <jats:italic>MSH2<\/jats:italic>\n                    alleles, i.e., E459K or R847C, did not confer selection of resistant colonies, confirming that not all mutations in the\n                    <jats:italic>MSH2<\/jats:italic>\n                    MMR pathway affect its function or generate a phenotype of resistance to antifungal drugs. The lack of drug resistance prevented any correlations from being drawn with respect to\n                    <jats:italic>MSH2<\/jats:italic>\n                    genotype.\n                  <\/jats:p>","DOI":"10.1128\/aac.00195-18","type":"journal-article","created":{"date-parts":[[2018,3,29]],"date-time":"2018-03-29T09:26:24Z","timestamp":1522315584000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":51,"title":["Absence of Azole or Echinocandin Resistance in Candida glabrata Isolates in India despite Background Prevalence of Strains with Defects in the DNA Mismatch Repair Pathway"],"prefix":"10.1128","volume":"62","author":[{"given":"Ashutosh","family":"Singh","sequence":"first","affiliation":[{"name":"Department of Medical Mycology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kelley R.","family":"Healey","sequence":"additional","affiliation":[{"name":"Public Health Research Institute, Rutgers Biomedical and Health Sciences, Newark, New Jersey, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Priyanka","family":"Yadav","sequence":"additional","affiliation":[{"name":"Department of Medical Mycology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gargi","family":"Upadhyaya","sequence":"additional","affiliation":[{"name":"Department of Medical Mycology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neelam","family":"Sachdeva","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Rajiv Gandhi Cancer Institute &amp; Research Center, Delhi, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Smita","family":"Sarma","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Medanta\u2014The Medicity, Gurgaon, Haryana, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anil","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Amrita Institute of Medical Sciences and Research Center, Amrita University, Ponekkara, Cochin, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bansidhar","family":"Tarai","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Max Hospital, New Delhi, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David S.","family":"Perlin","sequence":"additional","affiliation":[{"name":"Public Health Research Institute, Rutgers Biomedical and Health Sciences, Newark, New Jersey, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2028-7462","authenticated-orcid":false,"given":"Anuradha","family":"Chowdhary","sequence":"additional","affiliation":[{"name":"Department of Medical Mycology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi, India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00029-06"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.03255-14"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cit136"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/myc.12472"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00027-12"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciu407"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms11128"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.01995"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.02038"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkx480"},{"key":"e_1_3_3_12_2","volume-title":"CLSI document M27-A3","author":"Clinical and Laboratory Standards Institute","year":"2008","unstructured":"Clinical and Laboratory Standards Institute. 2008. 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