{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T17:03:31Z","timestamp":1790269411506,"version":"4.1.0"},"reference-count":40,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2012,7,1]],"date-time":"2012-07-01T00:00:00Z","timestamp":1341100800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2012,7]]},"abstract":"ABSTRACT<\/jats:title>\n \n Therapeutic treatment for systemic mycoses is severely hampered by the extremely limited number of antifungals. The difficulty of treatment of fungal infections in the central nervous system is further compounded by the poor central nervous system (CNS) penetration of most antifungals due to the blood-brain barrier. Only a few fungistatic azole drugs, such as fluconazole, show reasonable CNS penetration. Here we demonstrate that sertraline (Zoloft), the most frequently prescribed antidepressant, displays potent antifungal activity against\n Cryptococcus neoformans<\/jats:named-content>\n , the major causative agent of fungal meningitis. In\n in vitro<\/jats:italic>\n assays, this neurotropic drug is fungicidal to all natural\n Cryptococcus<\/jats:named-content>\n isolates tested at clinically relevant concentrations. Furthermore, sertraline interacts synergistically or additively with fluconazole against\n Cryptococcus<\/jats:named-content>\n . Importantly, consistent with our\n in vitro<\/jats:italic>\n observations, sertraline used alone reduces the brain fungal burden at an efficacy comparable to that of fluconazole in a murine model of systemic cryptococcosis. It works synergistically with fluconazole in reducing the fungal burden in brain, kidney, and spleen. In contrast to its potency against\n Cryptococcus<\/jats:named-content>\n , sertraline is less effective against strains of\n Candida<\/jats:named-content>\n species and its interactions with fluconazole against\n Candida<\/jats:named-content>\n strains are often antagonistic. Therefore, our data suggest the unique application of sertraline against cryptococcosis. To understand the antifungal mechanisms of sertraline, we screened a whole-genome deletion collection of\n Saccharomyces cerevisiae<\/jats:named-content>\n for altered sertraline susceptibility. Gene ontology analyses of selected mutations suggest that sertraline perturbs translation.\n In vitro<\/jats:italic>\n translation assays using fungal cell extracts show that sertraline inhibits protein synthesis. Taken together, our findings indicate the potential of adopting this antidepressant in treating cryptococcal meningitis.\n <\/jats:p>","DOI":"10.1128\/aac.00212-12","type":"journal-article","created":{"date-parts":[[2012,4,17]],"date-time":"2012-04-17T01:22:29Z","timestamp":1334625749000},"page":"3758-3766","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":186,"title":["The Antidepressant Sertraline Provides a Promising Therapeutic Option for Neurotropic Cryptococcal Infections"],"prefix":"10.1128","volume":"56","author":[{"given":"Bing","family":"Zhai","sequence":"first","affiliation":[{"name":"Department of Biology, Texas A&M University, College Station, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Biology, Texas A&M University, College Station, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linqi","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Biology, Texas A&M University, College Station, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew S.","family":"Sachs","sequence":"additional","affiliation":[{"name":"Department of Biology, Texas A&M University, College Station, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaorong","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Biology, Texas A&M University, College Station, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1192\/S0007125000291745","article-title":"The antidepressant effects of 5-HT uptake inhibitors","volume":"1989","author":"Aberg-Wistedt A","year":"1989","unstructured":"Aberg-WistedtA. 1989. 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Fluconazole penetration into cerebrospinal fluid: implications for treating fungal infections of the central nervous system. J. Infect. Dis. 157:178\u2013180.","journal-title":"J. Infect. Dis."},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1086\/507895"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/9781555818241"},{"key":"e_1_3_3_7_2","doi-asserted-by":"crossref","first-page":"1175","DOI":"10.1097\/00004583-200110000-00011","article-title":"Long-term sertraline treatment of children and adolescents with obsessive-compulsive disorder","volume":"40","author":"Cook EH","year":"2001","unstructured":"CookEH . 2001. Long-term sertraline treatment of children and adolescents with obsessive-compulsive disorder. J. Am. Acad. Child Adolesc. Psychiatry 40:1175\u20131181.","journal-title":"J. Am. Acad. Child Adolesc. 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