{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T14:48:53Z","timestamp":1790779733453,"version":"4.1.0"},"reference-count":69,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2018,5,1]],"date-time":"2018-05-01T00:00:00Z","timestamp":1525132800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2018,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Multidrug-resistant (MDR)\n            <jats:named-content content-type=\"genus-species\">Acinetobacter baumannii<\/jats:named-content>\n            strains appeared as serious emerging nosocomial pathogens in clinical environments and especially in intensive care units (ICUs).\n            <jats:named-content content-type=\"genus-species\">A. baumannii<\/jats:named-content>\n            strain K50, recovered from a hospitalized patient in Kuwait, exhibited resistance to carbapenems and additionally to ciprofloxacin, chloramphenicol, sulfonamides, amikacin, and gentamicin. Genome sequencing revealed that the strain possesses two plasmids, pK50a (79.6 kb) and pK50b (9.5 kb), and a 3.75-Mb chromosome.\n            <jats:named-content content-type=\"genus-species\">A. baumannii<\/jats:named-content>\n            K50 exhibits an average nucleotide identity (ANI) of 99.98% to the previously reported Iraqi clinical isolate AA-014, even though the latter strain lacked plasmid pK50a. Strain K50 belongs to sequence type 158 (ST158) (Pasteur scheme) and ST499 (Oxford scheme). Plasmid pK50a is a member of the Aci6 (replication group 6 [RG6]) group of\n            <jats:named-content content-type=\"genus-species\">Acinetobacter<\/jats:named-content>\n            plasmids and carries a conjugative transfer module and two antibiotic resistance gene regions. The transposon Tn\n            <jats:italic>2008<\/jats:italic>\n            carries the carbapenemase gene\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>OXA-23<\/jats:sub>\n            , whereas a class 1 integron harbors the resistance genes\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>GES-11<\/jats:sub>\n            ,\n            <jats:italic>aacA4<\/jats:italic>\n            ,\n            <jats:italic>dfrA7<\/jats:italic>\n            ,\n            <jats:italic>qacE<\/jats:italic>\n            \u0394\n            <jats:italic>1<\/jats:italic>\n            , and\n            <jats:italic>sul1<\/jats:italic>\n            , conferring resistance to all \u03b2-lactams and reduced susceptibility to carbapenems and resistance to aminoglycosides, trimethoprim, quaternary ammonium compounds, and sulfamethoxazole, respectively. The class 1 integron is flanked by MITEs (miniature inverted-repeat transposable elements) delimiting the element at its insertion site.\n          <\/jats:p>","DOI":"10.1128\/aac.00212-18","type":"journal-article","created":{"date-parts":[[2018,2,14]],"date-time":"2018-02-14T12:33:57Z","timestamp":1518611637000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":37,"title":["Complete Genome Sequencing of Acinetobacter baumannii Strain K50 Discloses the Large Conjugative Plasmid pK50a Encoding Carbapenemase OXA-23 and Extended-Spectrum \u03b2-Lactamase GES-11"],"prefix":"10.1128","volume":"62","author":[{"given":"Daniel","family":"Wibberg","sequence":"first","affiliation":[{"name":"Genome Research of Industrial Microorganisms, Center for Biotechnology (CeBiTec), Bielefeld University, Bielefeld, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ileana P.","family":"Salto","sequence":"additional","affiliation":[{"name":"IBBM (Instituto de Biotecnolog\u00eda y Biolog\u00eda Molecular), CCT-CONICET-La Plata, Departamento de Ciencias Biol\u00f3gicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felix G.","family":"Eikmeyer","sequence":"additional","affiliation":[{"name":"von Laer Stiftung, Bielefeld, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irena","family":"Maus","sequence":"additional","affiliation":[{"name":"Genome Research of Industrial Microorganisms, Center for Biotechnology (CeBiTec), Bielefeld University, Bielefeld, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anika","family":"Winkler","sequence":"additional","affiliation":[{"name":"Genome Research of Industrial Microorganisms, Center for Biotechnology (CeBiTec), Bielefeld University, Bielefeld, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrice","family":"Nordmann","sequence":"additional","affiliation":[{"name":"Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, Faculty of Science, University of Fribourg, Fribourg, Switzerland"},{"name":"INSERM European Unit (LEA Paris, France), University of Fribourg, Fribourg, Switzerland"},{"name":"Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Fribourg, Switzerland"},{"name":"HFR-H\u00f4pital Cantonal, Fribourg, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alfred","family":"P\u00fchler","sequence":"additional","affiliation":[{"name":"Genome Research of Industrial Microorganisms, Center for Biotechnology (CeBiTec), Bielefeld University, Bielefeld, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurent","family":"Poirel","sequence":"additional","affiliation":[{"name":"Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, Faculty of Science, University of Fribourg, Fribourg, Switzerland"},{"name":"INSERM European Unit (LEA Paris, France), University of Fribourg, Fribourg, Switzerland"},{"name":"Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Fribourg, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Schl\u00fcter","sequence":"additional","affiliation":[{"name":"Genome Research of Industrial Microorganisms, Center for Biotechnology (CeBiTec), Bielefeld University, Bielefeld, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1086\/529198"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/2049-632X.12125"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/iub.531"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-11-224"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01258-08"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2006.00195.x"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00072-09"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00871-10"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01384-12"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkt465"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2014.07.003"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgar.2016.03.005"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2015.07.007"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00142-17"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.03930-14"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw255"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0010034"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0058628"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp690"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv1239"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0062160"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.carres.2015.03.016"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1104404108"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0107833"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkx343"},{"key":"e_1_3_3_27_2","first-page":"113","article-title":"Organisation and evolution of the tol-pal gene cluster","volume":"3","author":"Sturgis JN","year":"2001","unstructured":"Sturgis JN. 2001. Organisation and evolution of the tol-pal gene cluster. J Mol Microbiol Biotechnol 3:113\u2013122.","journal-title":"J Mol Microbiol Biotechnol"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109696200"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2009.01659.x"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw387"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2008.01191.x"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.26204-0"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dku188"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00834-08"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00542-10"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dku527"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01661-10"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.00163"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.02176-12"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2009.00168.x"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.4161\/mge.24263"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkv102"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00131-15"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.4161\/mge.22967"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00056-13"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01033-09"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00692-13"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkt454"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00244-11"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.plasmid.2012.01.011"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.plasmid.2012.11.001"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02784-14"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12941-017-0208-y"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkv072"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkq095"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1159\/000448785"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1016\/0147-619X(84)90063-5"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2011.01.010"},{"key":"e_1_3_3_59_2","doi-asserted-by":"publisher","DOI":"10.1128\/genomeA.00850-15"},{"key":"e_1_3_3_60_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00124-16"},{"key":"e_1_3_3_61_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu153"},{"key":"e_1_3_3_62_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkg312"},{"key":"e_1_3_3_63_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-12-444"},{"key":"e_1_3_3_64_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2012.03.007"},{"key":"e_1_3_3_65_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0409727102"},{"key":"e_1_3_3_66_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0144769"},{"key":"e_1_3_3_67_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01310-13"},{"key":"e_1_3_3_68_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.01445"},{"key":"e_1_3_3_69_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0066584"},{"key":"e_1_3_3_70_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-7-433"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.00212-18","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.00212-18","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T21:14:15Z","timestamp":1645478055000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.00212-18"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,5]]},"references-count":69,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2018,5]]}},"alternative-id":["10.1128\/AAC.00212-18"],"URL":"https:\/\/doi.org\/10.1128\/aac.00212-18","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,5]]},"assertion":[{"value":"2018-02-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-02-03","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-04-26","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00212-18"}}