{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T12:12:43Z","timestamp":1789647163053,"version":"4.0.0"},"reference-count":36,"publisher":"American Society for Microbiology","license":[{"start":{"date-parts":[[2025,5,23]],"date-time":"2025-05-23T00:00:00Z","timestamp":1747958400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,5,23]],"date-time":"2025-05-23T00:00:00Z","timestamp":1747958400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81672063"],"award-info":[{"award-number":["81672063"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81971973"],"award-info":[{"award-number":["81971973"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82270005"],"award-info":[{"award-number":["82270005"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81800003"],"award-info":[{"award-number":["81800003"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007219","name":"Natural Science Foundation of Shanghai Municipality","doi-asserted-by":"publisher","award":["22ZR1452000"],"award-info":[{"award-number":["22ZR1452000"]}],"id":[{"id":"10.13039\/100007219","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018625","name":"Science and Technology Innovation Plan Of Shanghai Science and Technology Commission","doi-asserted-by":"publisher","award":["23S11901400"],"award-info":[{"award-number":["23S11901400"]}],"id":[{"id":"10.13039\/501100018625","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Shanghai Municipal Health Commission Excellence Project","award":["20244Z0017"],"award-info":[{"award-number":["20244Z0017"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Antibiotic therapy for\n                      <jats:italic toggle=\"yes\">Mycobacterium abscessus<\/jats:italic>\n                      infections is challenging due to resistance of the organism to many clinically available antimicrobials. Here, the efficacy of NITD-916, an enoyl-ACP reductase inhibitor, in preventing\n                      <jats:italic toggle=\"yes\">M. abscessus<\/jats:italic>\n                      growth\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                      is demonstrated. The minimal inhibitory concentrations (MICs) of NITD-916 for 12 non-tuberculosis mycobacteria (NTM) reference strains and a collection of 194 clinical\n                      <jats:italic toggle=\"yes\">M. abscessus<\/jats:italic>\n                      isolates were determined using the broth microdilution method. Compatibility of NITD-916 with 10 clinically important antibiotics was ascertained by checkerboard assay. The activity of NITD-916 against\n                      <jats:italic toggle=\"yes\">M. abscessus<\/jats:italic>\n                      growing in cultured macrophages was also evaluated. Finally, the potency of NITD-916\n                      <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                      was determined in a mouse model that mimicked an acute pulmonary\n                      <jats:italic toggle=\"yes\">M. abscessus<\/jats:italic>\n                      infection. NITD-916 was bacteriostatic for\n                      <jats:italic toggle=\"yes\">M. abscessus<\/jats:italic>\n                      replicating\n                      <jats:italic toggle=\"yes\">in vitro,<\/jats:italic>\n                      expressing a MIC\n                      <jats:sub>50<\/jats:sub>\n                      of 0.125 mg\/L and a MIC\n                      <jats:sub>90<\/jats:sub>\n                      of 1 mg\/L against the screened clinical isolates. Furthermore, NITD-916 synergized with clarithromycin in treating 2 out of 5 subsp.\n                      <jats:italic toggle=\"yes\">massiliense<\/jats:italic>\n                      strains. NITD-916 was active against\n                      <jats:italic toggle=\"yes\">M. abscessus<\/jats:italic>\n                      replicating in both cultured macrophages and infected mice. The administration of 100 mg\/kg NITD-916 for 14 days resulted in a 5.6 log\n                      <jats:sub>10<\/jats:sub>\n                      colony-forming units (CFUs) reduction in the bacterial load in mouse lung tissue. NITD-916 is active against\n                      <jats:italic toggle=\"yes\">M. abscessus in vitro<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                      and may be used potentially to treat\n                      <jats:italic toggle=\"yes\">M. abscessus<\/jats:italic>\n                      diseases.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/aac.00249-25","type":"journal-article","created":{"date-parts":[[2025,5,23]],"date-time":"2025-05-23T09:00:27Z","timestamp":1747990827000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["An enoyl-ACP reductase inhibitor, NITD-916, expresses anti-\n                    <i>Mycobacterium abscessus<\/i>\n                    activity"],"prefix":"10.1128","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-0034-8230","authenticated-orcid":false,"given":"Yaping","family":"Jia","sequence":"first","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine, Shanghai Pulmonary Hospital, School of Medicine,Tongji University","place":["Shanghai, China"]},{"name":"School of Medicine, Tongji University","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junsheng","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine, Shanghai Pulmonary Hospital, School of Medicine,Tongji University","place":["Shanghai, China"]},{"name":"School of Medicine, Tongji University","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhili","family":"Tan","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine, Shanghai Pulmonary Hospital, School of Medicine,Tongji University","place":["Shanghai, China"]},{"name":"School of Medicine, Tongji University","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anqi","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine, Shanghai Pulmonary Hospital, School of Medicine,Tongji University","place":["Shanghai, China"]},{"name":"School of Medicine, Tongji University","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siyuan","family":"He","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine, Shanghai Pulmonary Hospital, School of Medicine,Tongji University","place":["Shanghai, China"]},{"name":"School of Medicine, Tongji University","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yani","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine, Shanghai Pulmonary Hospital, School of Medicine,Tongji University","place":["Shanghai, China"]},{"name":"School of Medicine, Tongji University","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine, Shanghai Pulmonary Hospital, School of Medicine,Tongji University","place":["Shanghai, China"]},{"name":"School of Medicine, Tongji University","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhemin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine, Shanghai Pulmonary Hospital, School of Medicine,Tongji 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China"]},{"name":"Shanghai Key Laboratory of Tuberculosis, Shanghai Pulmonary Hospital, School of Medicine,Tongji University","place":["Shanghai, 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CLSI standard document M24. Susceptibility testing of mycobacteria, Nocardia spp., and other aerobic actinomycetes. 3rd ed. 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