{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T09:12:18Z","timestamp":1790500338291,"version":"4.1.0"},"reference-count":38,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2006,10,1]],"date-time":"2006-10-01T00:00:00Z","timestamp":1159660800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2006,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The structure of the macrolide efflux genetic assembly (mega) element, its genomic locations, and its association with other resistance determinants and genetic elements were investigated in 16\n            <jats:italic>Streptococcus pneumoniae<\/jats:italic>\n            isolates carrying\n            <jats:italic>mef<\/jats:italic>\n            (E), of which 1 isolate also carried\n            <jats:italic>tet<\/jats:italic>\n            (M) and 4 isolates also carried\n            <jats:italic>tet<\/jats:italic>\n            (M) and\n            <jats:italic>erm<\/jats:italic>\n            (B). All isolates carried a mega element of similar size and structure that included the operon\n            <jats:italic>mef<\/jats:italic>\n            (E)-\n            <jats:italic>msr<\/jats:italic>\n            (D) encoding the efflux transport system. Among tetracycline-susceptible isolates, six different integration sites were identified, five of which were recognized inside open reading frames present in the R6 genome. In the five isolates also carrying\n            <jats:italic>tet<\/jats:italic>\n            (M), mega was inserted in different genetic contexts. In one isolate, it was part of previously described Tn\n            <jats:italic>916<\/jats:italic>\n            -like element Tn\n            <jats:italic>2009<\/jats:italic>\n            . In another isolate, mega was inserted in a transposon similar to Tn\n            <jats:italic>2009<\/jats:italic>\n            that also included an\n            <jats:italic>erm<\/jats:italic>\n            (B) element. This new composite transposon was designated Tn\n            <jats:italic>2010<\/jats:italic>\n            . Neither Tn\n            <jats:italic>2009<\/jats:italic>\n            nor Tn\n            <jats:italic>2010<\/jats:italic>\n            could be transferred by conjugation to pneumococcal or enterococcal recipients. In the three isolates in which mega was not physically linked with\n            <jats:italic>tet<\/jats:italic>\n            (M), this gene was associated with\n            <jats:italic>erm<\/jats:italic>\n            (B) in transposon Tn\n            <jats:italic>3872<\/jats:italic>\n            , a Tn\n            <jats:italic>916<\/jats:italic>\n            -like element. Homologies between the chromosomal insertions of these composite transposons and sequences of multidrug-resistant pneumococcal genomes in the databases indicate the presence of preferential sites for the integration of composite Tn\n            <jats:italic>916<\/jats:italic>\n            -like elements carrying multiple resistance determinants in\n            <jats:italic>S. pneumoniae<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1128\/aac.00277-06","type":"journal-article","created":{"date-parts":[[2006,9,27]],"date-time":"2006-09-27T17:13:29Z","timestamp":1159377209000},"page":"3361-3366","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":61,"title":["The\n            <i>mef<\/i>\n            (E)-Carrying Genetic Element (mega) of\n            <i>Streptococcus pneumoniae<\/i>\n            : Insertion Sites and Association with Other Genetic Elements"],"prefix":"10.1128","volume":"50","author":[{"given":"Maria","family":"Del Grosso","sequence":"first","affiliation":[{"name":"Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanita\u0300, Rome"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Romina","family":"Camilli","sequence":"additional","affiliation":[{"name":"Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanita\u0300, Rome"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Iannelli","sequence":"additional","affiliation":[{"name":"LAMMB, Dipartimento di Biologia Molecolare, Universita\u0300 di Siena, Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gianni","family":"Pozzi","sequence":"additional","affiliation":[{"name":"LAMMB, Dipartimento di Biologia Molecolare, Universita\u0300 di Siena, Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annalisa","family":"Pantosti","sequence":"additional","affiliation":[{"name":"Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanita\u0300, Rome"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.10.4203-4209.2005"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.9.3313-3318.2002"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0147-619X(87)90009-6"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(89)90759-9"},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1179\/joc.2005.17.5.502","volume":"17","year":"2005","unstructured":"Cascone, C., M. 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