{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T13:54:18Z","timestamp":1791986058640,"version":"4.4.0"},"reference-count":50,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2015,7,1]],"date-time":"2015-07-01T00:00:00Z","timestamp":1435708800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2015,7]]},"abstract":"ABSTRACT<\/jats:title>\n \n Sensititre YeastOne (SYO) is an affordable alternative to the Clinical and Laboratory Standards Institute (CLSI) reference method for antifungal susceptibility testing. In this study, the MICs of yeast isolates from 1,214 bloodstream infection episodes, generated by SYO during hospital laboratory activity (January 2005 to December 2013), were reanalyzed using current CLSI clinical breakpoints\/epidemiological cutoff values to assign susceptibility (or the wild-type [WT] phenotype) to systemic antifungal agents. Excluding\n Candida albicans<\/jats:named-content>\n (57.4% of all isolates [\n n<\/jats:italic>\n = 1,250]), the most predominant species were\n Candida parapsilosis<\/jats:named-content>\n complex (20.9%),\n Candida tropicalis<\/jats:named-content>\n (8.2%),\n Candida glabrata<\/jats:named-content>\n (6.4%),\n Candida guilliermondii<\/jats:named-content>\n (1.6%), and\n Candida krusei<\/jats:named-content>\n (1.3%). Among the non-\n Candida<\/jats:named-content>\n species (1.9%), 7 were\n Cryptococcus neoformans<\/jats:named-content>\n and 17 were other species, mainly\n Rhodotorula<\/jats:named-content>\n species. Over 97% of\n Candida<\/jats:named-content>\n isolates were susceptible (WT phenotype) to amphotericin B and flucytosine. Rates of susceptibility (WT phenotype) to fluconazole, itraconazole, and voriconazole were 98.7% in\n C. albicans<\/jats:named-content>\n , 92.3% in the\n C. parapsilosis<\/jats:named-content>\n complex, 96.1% in\n C. tropicalis<\/jats:named-content>\n , 92.5% in\n C. glabrata<\/jats:named-content>\n , 100% in\n C. guilliermondii<\/jats:named-content>\n , and 100% (excluding fluconazole) in\n C. krusei<\/jats:named-content>\n . The fluconazole-resistant isolates consisted of 6\n C. parapsilosis<\/jats:named-content>\n complex isolates, 3\n C. glabrata<\/jats:named-content>\n isolates, 2\n C. albicans<\/jats:named-content>\n isolates, 2\n C. tropicalis<\/jats:named-content>\n isolates, and 1\n Candida<\/jats:named-content>\n lusitaniae<\/jats:named-content>\n isolate. Of the non-\n Candida<\/jats:named-content>\n isolates, 2\n C. neoformans<\/jats:named-content>\n isolates had the non-WT phenotype for susceptibility to fluconazole, whereas\n Rhodotorula<\/jats:named-content>\n isolates had elevated azole MICs. Overall, 99.7% to 99.8% of\n Candida<\/jats:named-content>\n isolates were susceptible (WT phenotype) to echinocandins, but 3 isolates were nonsusceptible (either intermediate or resistant) to caspofungin (\n C. albicans<\/jats:named-content>\n ,\n C. guilliermondii<\/jats:named-content>\n , and\n C. krusei<\/jats:named-content>\n ), anidulafungin (\n C. albicans<\/jats:named-content>\n and\n C. guilliermondii<\/jats:named-content>\n ), and micafungin (\n C. albicans<\/jats:named-content>\n ). However, when the intrinsically resistant non-\n Candida<\/jats:named-content>\n isolates were included, the rate of echinocandin nonsusceptibility reached 1.8%. In summary, the SYO method proved to be able to detect yeast species showing antifungal resistance or reduced susceptibility.\n <\/jats:p>","DOI":"10.1128\/aac.00285-15","type":"journal-article","created":{"date-parts":[[2015,4,21]],"date-time":"2015-04-21T02:31:49Z","timestamp":1429583509000},"page":"3944-3955","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":70,"title":["Antifungal Susceptibility Profiles of Bloodstream Yeast Isolates by Sensititre YeastOne over Nine Years at a Large Italian Teaching Hospital"],"prefix":"10.1128","volume":"59","author":[{"given":"Brunella","family":"Posteraro","sequence":"first","affiliation":[{"name":"Institute of Public Health (Section of Hygiene), Universit\u00e0 Cattolica del Sacro Cuore, Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teresa","family":"Spanu","sequence":"additional","affiliation":[{"name":"Institute of Microbiology, Universit\u00e0 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