{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T00:56:12Z","timestamp":1790470572334,"version":"4.1.0"},"reference-count":41,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2017,8,1]],"date-time":"2017-08-01T00:00:00Z","timestamp":1501545600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            AxyXY-OprZ is an RND-type efflux system that confers innate aminoglycoside resistance to\n            <jats:named-content content-type=\"genus-species\">Achromobacter<\/jats:named-content>\n            spp. We investigated here a putative TetR family transcriptional regulator encoded by the\n            <jats:italic>axyZ<\/jats:italic>\n            gene located upstream of\n            <jats:italic>axyXY-oprZ<\/jats:italic>\n            . An in-frame\n            <jats:italic>axyZ<\/jats:italic>\n            gene deletion assay led to increased MICs of antibiotic substrates of the efflux system, including aminoglycosides, cefepime, fluoroquinolones, tetracyclines, and erythromycin, indicating that the product of\n            <jats:italic>axyZ<\/jats:italic>\n            negatively regulates expression of\n            <jats:italic>axyXY-oprZ<\/jats:italic>\n            . Moreover, we identified an amino acid substitution at position 29 of AxyZ (V29G) in a clinical\n            <jats:named-content content-type=\"genus-species\">Achromobacter<\/jats:named-content>\n            strain that occurred during the course of chronic respiratory tract colonization in a cystic fibrosis (CF) patient. This substitution, also detected in three other strains exposed\n            <jats:italic>in vitro<\/jats:italic>\n            to tobramycin, led to an increase in the\n            <jats:italic>axyY<\/jats:italic>\n            transcription level (5- to 17-fold) together with an increase in antibiotic resistance level. This overproduction of AxyXY-OprZ is the first description of antibiotic resistance acquisition due to modification of a chromosomally encoded mechanism in\n            <jats:named-content content-type=\"genus-species\">Achromobacter<\/jats:named-content>\n            and might have an impact on the management of infected CF patients. Indeed, tobramycin is widely used for aerosol therapy within this population, and we have demonstrated that it easily selects mutants with increased MICs of not only aminoglycosides but also fluoroquinolones, cefepime, and tetracyclines.\n          <\/jats:p>","DOI":"10.1128\/aac.00290-17","type":"journal-article","created":{"date-parts":[[2017,6,6]],"date-time":"2017-06-06T00:30:26Z","timestamp":1496709026000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":26,"title":["Role of AxyZ Transcriptional Regulator in Overproduction of AxyXY-OprZ Multidrug Efflux System in Achromobacter Species Mutants Selected by Tobramycin"],"prefix":"10.1128","volume":"61","author":[{"given":"Julien","family":"Bador","sequence":"first","affiliation":[{"name":"Laboratoire de Bact\u00e9riologie, H\u00f4pital Universitaire de Dijon, Dijon, France"},{"name":"UMR 6249 CNRS Chrono-environnement, Universit\u00e9 de Bourgogne Franche-Comt\u00e9, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Neuwirth","sequence":"additional","affiliation":[{"name":"Laboratoire de Bact\u00e9riologie, H\u00f4pital Universitaire de Dijon, Dijon, France"},{"name":"UMR 6249 CNRS Chrono-environnement, Universit\u00e9 de Bourgogne Franche-Comt\u00e9, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nad\u00e8ge","family":"Grangier","sequence":"additional","affiliation":[{"name":"Laboratoire de Bact\u00e9riologie, H\u00f4pital Universitaire de Dijon, Dijon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie","family":"Muniz","sequence":"additional","affiliation":[{"name":"Laboratoire de Bact\u00e9riologie, H\u00f4pital Universitaire de Dijon, Dijon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leslie","family":"Germ\u00e9","sequence":"additional","affiliation":[{"name":"Laboratoire de Bact\u00e9riologie, H\u00f4pital Universitaire de Dijon, Dijon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e9r\u00e9my","family":"Bonnet","sequence":"additional","affiliation":[{"name":"Laboratoire de Bact\u00e9riologie, H\u00f4pital Universitaire de Dijon, Dijon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vignesh-Guru","family":"Pillay","sequence":"additional","affiliation":[{"name":"Laboratoire de Bact\u00e9riologie, H\u00f4pital Universitaire de Dijon, Dijon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Llanes","sequence":"additional","affiliation":[{"name":"UMR 6249 CNRS Chrono-environnement, Universit\u00e9 de Bourgogne Franche-Comt\u00e9, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire","family":"de Curraize","sequence":"additional","affiliation":[{"name":"Laboratoire de Bact\u00e9riologie, H\u00f4pital Universitaire de Dijon, Dijon, France"},{"name":"UMR 6249 CNRS Chrono-environnement, Universit\u00e9 de Bourgogne Franche-Comt\u00e9, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucie","family":"Amoureux","sequence":"additional","affiliation":[{"name":"Laboratoire de Bact\u00e9riologie, H\u00f4pital Universitaire de Dijon, Dijon, France"},{"name":"UMR 6249 CNRS Chrono-environnement, Universit\u00e9 de Bourgogne Franche-Comt\u00e9, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcf.2015.03.012"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.1971.tb00607.x"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.1998.tb02306.x"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.02609-0"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.02698-0"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.025304-0"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.syapm.2012.10.003"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.000872"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.syapm.2013.06.005"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.001254"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcf.2012.10.002"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcf.2015.12.009"},{"key":"e_1_3_1_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcf.2009.10.005"},{"key":"e_1_3_1_15_2","volume-title":"Antibiotic treatment for cystic fibrosis","author":"UK Cystic Fibrosis Trust Antibiotic Working Group","year":"2009","unstructured":"UK Cystic Fibrosis Trust Antibiotic Working Group. 2009. 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