{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T13:52:39Z","timestamp":1791381159618,"version":"4.3.3"},"reference-count":50,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:00:00Z","timestamp":1791331200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:00:00Z","timestamp":1791331200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2026,10,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Talaromyces marneffei<\/jats:italic>\n                      is a thermally dimorphic fungus causing life-threatening systemic mycosis in immunocompromised patients. The emergence of azole-resistant clinical isolates, especially those with reduced susceptibility to voriconazole (VOC) and fluconazole (FLC), presents a critical therapeutic challenge. Although target-gene alterations and efflux-related mechanisms have been reported in pathogenic fungi, the broader molecular basis distinguishing high-minimum-inhibitory concentration (MIC) and low-MIC\n                      <jats:italic toggle=\"yes\">T. marneffei<\/jats:italic>\n                      clinical isolates remains incompletely defined. Here, we performed integrated transcriptomic (RNA-seq), proteomic (DIA-LC-MS\/MS), and RT-qPCR analyses comparing four resistant and four susceptible\n                      <jats:italic toggle=\"yes\">T. marneffei<\/jats:italic>\n                      clinical isolates. Differentially expressed genes and proteins were identified, functionally interpreted, and integrated to prioritize resistance-associated molecular candidates. The multi-omics results supported a constitutive, multitiered resistance-associated state involving transport and cell wall processes, redox-detoxification, mitochondrial metabolism, and stress regulatory adaptation. Signals involving coilin, CatA, and other discriminant features contributed to the multi-omics prioritization layer. RT-qPCR supported a 17-gene panel associated with transport, redox-detoxification, mitochondrial metabolism, and stress regulation. Five genes,\n                      <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PMAA_008970\" xlink:type=\"simple\">PMAA_008970<\/jats:ext-link>\n                      ,\n                      <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PMAA_058960\" xlink:type=\"simple\">PMAA_058960<\/jats:ext-link>\n                      ,\n                      <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PMAA_070510\" xlink:type=\"simple\">PMAA_070510<\/jats:ext-link>\n                      ,\n                      <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PMAA_088690\" xlink:type=\"simple\">PMAA_088690<\/jats:ext-link>\n                      , and\n                      <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PMAA_092180\" xlink:type=\"simple\">PMAA_092180<\/jats:ext-link>\n                      , showed statistically significant R\/S differences, while the remaining panel members were detectable and trend-supporting within the same functional framework. These findings refine a hierarchical azole-resistance model in\n                      <jats:italic toggle=\"yes\">T. marneffei<\/jats:italic>\n                      and provide a focused candidate panel for future mechanistic validation.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/aac.00293-26","type":"journal-article","created":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T16:25:21Z","timestamp":1788366321000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Comparative transcriptome and proteome analysis of resistant and susceptible\n                    <i>Talaromyces marneffei<\/i>\n                    clinical isolates reveals a systemic multitiered defense network against azoles"],"prefix":"10.1128","volume":"70","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7809-5527","authenticated-orcid":true,"given":"Zheng","family":"Yanqing","sequence":"first","affiliation":[{"name":"The Fourth People`s Hospital of Nanning","place":["Nanning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Gaoyuan","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03dveyr97","id-type":"ROR","asserted-by":"publisher"}],"name":"Guangxi Key Laboratory of Mycosis Prevention and Treatment, Department of Dermatology and Venereology, The First Affiliated Hospital of Guangxi Medical University","place":["Nanning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pang","family":"Xiaofeng","sequence":"additional","affiliation":[{"name":"The Fourth People`s Hospital of Nanning","place":["Nanning, 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