{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T16:55:24Z","timestamp":1790355324669,"version":"4.1.0"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2017,7,1]],"date-time":"2017-07-01T00:00:00Z","timestamp":1498867200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"National Major Project","award":["2014CB542800"],"award-info":[{"award-number":["2014CB542800"]}]},{"name":"National Major Project","award":["2013CB911100"],"award-info":[{"award-number":["2013CB911100"]}]},{"name":"Tsinghua University Initiative Scientific Research Program","award":["20131089228"],"award-info":[{"award-number":["20131089228"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31370733"],"award-info":[{"award-number":["31370733"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81322023"],"award-info":[{"award-number":["81322023"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2017,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Hand-foot-and-mouth disease (HFMD), caused by enterovirus, is a threat to public health worldwide. To date, enterovirus 71 (EV71) has been one of the major causative agents of HFMD in the Pacific-Asia region, and outbreaks with EV71 cause millions of infections. However, no drug is currently available for clinical therapeutics. In our previous works, we developed a set of protease inhibitors (PIs) targeting the EV71 3C protease (3C\n            <jats:sup>pro<\/jats:sup>\n            ). Among these are NK-1.8k and NK-1.9k, which have various active groups and high potencies and selectivities. In the study described here, we determined the structures of the PI NK-1.8k in complex with wild-type (WT) and drug-resistant EV71 3C\n            <jats:sup>pro<\/jats:sup>\n            . Comparison of these structures with the structure of unliganded EV71 3C\n            <jats:sup>pro<\/jats:sup>\n            and its complex with AG7088 indicated that the mutation of N69 to a serine residue destabilized the S2 pocket. Thus, the mutation influenced the cleavage activity of EV71 3C\n            <jats:sup>pro<\/jats:sup>\n            and the inhibitory activity of NK-1.8k in an\n            <jats:italic>in vitro<\/jats:italic>\n            protease assay and highlighted that site 69 is an additional key site for PI design. More information for the optimization of the P1\u2032 to P4 groups of PIs was also obtained from these structures. Together with the results of our previous works, these in-depth results elucidate the inhibitory mechanism of PIs and shed light to develop PIs for the clinical treatment of infections caused by EV71 and other enteroviruses.\n          <\/jats:p>","DOI":"10.1128\/aac.00298-17","type":"journal-article","created":{"date-parts":[[2017,5,2]],"date-time":"2017-05-02T00:20:41Z","timestamp":1493684441000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":22,"title":["Structure of the Enterovirus 71 3C Protease in Complex with NK-1.8k and Indications for the Development of Antienterovirus Protease Inhibitor"],"prefix":"10.1128","volume":"61","author":[{"given":"Yaxin","family":"Wang","sequence":"first","affiliation":[{"name":"College of Pharmacy &amp; State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Cao","sequence":"additional","affiliation":[{"name":"College of Pharmacy &amp; State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yangyang","family":"Zhai","sequence":"additional","affiliation":[{"name":"College of Pharmacy &amp; State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng","family":"Yin","sequence":"additional","affiliation":[{"name":"College of Pharmacy &amp; State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuna","family":"Sun","sequence":"additional","affiliation":[{"name":"National Laboratory of Macromolecules, Institute of Biophysics, Chinese Academy of Science, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luqing","family":"Shang","sequence":"additional","affiliation":[{"name":"College of Pharmacy &amp; State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1301.020112"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2006-3331"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2013.12.008"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1218713"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02733-12"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01712-12"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1024071514438"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2011.03.007"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1021\/cr950025u"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.2174\/092986706776055733"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1021\/ci200029y"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/s41048-015-0006-z"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.04698-14"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02274-15"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2014.10.009"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00049-15"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2016.08.064"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.5b01013"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2008.06.015"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.20.11000"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.10.2444"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.05107-11"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00787-11"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(97)76066-X"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0021889807021206"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444904019158"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444902016657"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444909042073"},{"key":"e_1_3_2_30_2","volume-title":"The PyMOL molecular graphics system","author":"DeLano W","year":"2002","unstructured":"DeLano W . 2002. 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