{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T20:54:10Z","timestamp":1790801650083,"version":"4.1.0"},"reference-count":37,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2011,11,1]],"date-time":"2011-11-01T00:00:00Z","timestamp":1320105600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2011,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The 16S rRNA methyltransferase ArmA is a worldwide emerging determinant that confers high-level resistance to most clinically relevant aminoglycosides. We report here the identification and characterization of a multidrug-resistant<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Salmonella enterica<\/jats:named-content>subspecies I.4,12:i:\u2212 isolate recovered from chicken meat sampled in a supermarket on February 2009 in La Reunion, a French island in the Indian Ocean. Susceptibility testing showed an unusually high-level resistance to gentamicin, as well as to ampicillin, expanded-spectrum cephalosporins and amoxicillin-clavulanate. Molecular analysis of the 16S rRNA methyltransferases revealed presence of the<jats:italic>armA<\/jats:italic>gene, together with<jats:italic>bla<\/jats:italic><jats:sub>TEM-1<\/jats:sub>,<jats:italic>bla<\/jats:italic><jats:sub>CMY-2<\/jats:sub>, and<jats:italic>bla<\/jats:italic><jats:sub>CTX-M-3<\/jats:sub>. All of these genes could be transferred<jats:italic>en bloc<\/jats:italic>through conjugation into<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Escherichia coli<\/jats:named-content>at a frequency of 10<jats:sup>\u22125<\/jats:sup>CFU\/donor. Replicon typing and S1 pulsed-field gel electrophoresis revealed that the<jats:italic>armA<\/jats:italic>gene was borne on an \u223c150-kb broad-host-range IncP plasmid, pB1010. To elucidate how<jats:italic>armA<\/jats:italic>had integrated in pB1010, a PCR mapping strategy was developed for Tn<jats:italic>1548<\/jats:italic>, the genetic platform for<jats:italic>armA.<\/jats:italic>The gene was embedded in a Tn<jats:italic>1548<\/jats:italic>-like structure, albeit with a deletion of the macrolide resistance genes, and an IS<jats:italic>26<\/jats:italic>was inserted within the<jats:italic>mel<\/jats:italic>gene. To our knowledge, this is the first report of ArmA methyltransferase in food, showing a novel route of transmission for this resistance determinant. Further surveillance in food-borne bacteria will be crucial to determine the role of food in the spread of 16S rRNA methyltransferase genes worldwide.<\/jats:p>","DOI":"10.1128\/aac.00308-11","type":"journal-article","created":{"date-parts":[[2011,8,23]],"date-time":"2011-08-23T03:06:23Z","timestamp":1314068783000},"page":"5262-5266","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":27,"title":["ArmA Methyltransferase in a Monophasic Salmonella enterica Isolate from Food"],"prefix":"10.1128","volume":"55","author":[{"given":"Sophie A.","family":"Granier","sequence":"first","affiliation":[{"name":"ANSES, Laboratoire de S\u00e9curit\u00e9 des Aliments, Maisons-Alfort, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Hidalgo","sequence":"additional","affiliation":[{"name":"Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, Spain"},{"name":"Centro de Vigilancia Sanitaria Veterinaria (VISAVET), Universidad Complutense de Madrid, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alvaro","family":"San Millan","sequence":"additional","affiliation":[{"name":"Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, Spain"},{"name":"Centro de Vigilancia Sanitaria Veterinaria (VISAVET), Universidad Complutense de Madrid, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose Antonio","family":"Escudero","sequence":"additional","affiliation":[{"name":"Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, Spain"},{"name":"Centro de Vigilancia Sanitaria Veterinaria (VISAVET), Universidad Complutense de Madrid, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Belen","family":"Gutierrez","sequence":"additional","affiliation":[{"name":"Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, Spain"},{"name":"Centro de Vigilancia Sanitaria Veterinaria (VISAVET), Universidad Complutense de Madrid, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Brisabois","sequence":"additional","affiliation":[{"name":"ANSES, Laboratoire de S\u00e9curit\u00e9 des Aliments, Maisons-Alfort, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno","family":"Gonzalez-Zorn","sequence":"additional","affiliation":[{"name":"Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, Spain"},{"name":"Centro de Vigilancia Sanitaria Veterinaria (VISAVET), Universidad Complutense de Madrid, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2008.0812"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.19.5725-5732.2001"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-11-151"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2005.03.018"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkm065"},{"key":"e_1_3_2_7_2","volume-title":"M2\u2013A9: performance standards for antimicrobial disk susceptibility tests; approved standards","author":"Clinical and Laboratory Standards Institute","year":"2006","unstructured":"Clinical and Laboratory Standards Institute. 2006. 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