{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T21:51:10Z","timestamp":1791323470399,"version":"4.3.1"},"reference-count":16,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2015,6,1]],"date-time":"2015-06-01T00:00:00Z","timestamp":1433116800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2015,6]]},"abstract":"ABSTRACT<\/jats:title>\n \n The OqxAB efflux pump, a plasmid-mediated quinolone resistance (PMQR) determinant, has become increasingly prevalent among members of\n Enterobacteriaceae<\/jats:named-content>\n over the past decade. To investigate the evolution and dissemination routes of the\n oqxAB<\/jats:italic>\n operon, we assessed the prevalence of\n oqxAB<\/jats:italic>\n -like elements among various Gram-negative bacterial species and analyzed the genotypic and phenotypic characteristics of organisms harboring such elements. With a comprehensive genotyping approach, a chromosome-based\n oqxAB<\/jats:italic>\n operon was detectable in all\n Klebsiella pneumoniae<\/jats:named-content>\n strains tested, including organisms isolated before the year 1984. Sequence and phylogenetic analyses confirmed that the\n oqxAB<\/jats:italic>\n operon in\n K. pneumoniae<\/jats:named-content>\n isolates was genetically closest to their plasmid-borne counterparts recoverable only from\n Escherichia coli<\/jats:named-content>\n and\n Salmonella<\/jats:named-content>\n isolates collected from the year 2003 onward. Chromosomal elements with much lower sequence homology were also found among the\n Enterobacter<\/jats:named-content>\n spp. but not other Gram-negative species. Contrary to the quinolone resistance phenotypes which were consistently observable among organisms with\n oqxAB<\/jats:italic>\n -harboring plasmids, chromosomal\n oqxAB<\/jats:italic>\n elements generally did not confer quinolone resistance, except for\n K. pneumoniae<\/jats:named-content>\n strains, which exhibited a typical\n oqxAB<\/jats:italic>\n -mediated phenotype characterized by cross-resistance to olaquindox, chloramphenicol, and the quinolones. Gene expression analysis illustrated that such phenotypes were due to elevated expression of the chromosomal\n oqxAB<\/jats:italic>\n operon. Furthermore, transposition of the\n oqxAB<\/jats:italic>\n operon from the bacterial chromosome to plasmids was found to result in a >80-fold increase in the level of expression of the OqxAB pump, confirming its status as the first constitutively expressed efflux system located in bacterial mobile elements.\n <\/jats:p>","DOI":"10.1128\/aac.00310-15","type":"journal-article","created":{"date-parts":[[2015,3,24]],"date-time":"2015-03-24T02:32:47Z","timestamp":1427164367000},"page":"3290-3297","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":89,"title":["Evolution and Dissemination of OqxAB-Like Efflux Pumps, an Emerging Quinolone Resistance Determinant among Members of Enterobacteriaceae"],"prefix":"10.1128","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5472-8337","authenticated-orcid":false,"given":"Marcus Ho Yin","family":"Wong","sequence":"first","affiliation":[{"name":"Shenzhen Key Laboratory for Food Biological Safety Control, Food Safety and Technology Research Centre, Hong Kong PolyU Shenzhen Research Institute, Shenzhen, People's Republic of China"},{"name":"State Key Laboratory of Chirosciences, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward Wai Chi","family":"Chan","sequence":"additional","affiliation":[{"name":"Shenzhen Key Laboratory for Food Biological Safety Control, Food Safety and Technology Research Centre, Hong Kong PolyU Shenzhen Research Institute, Shenzhen, People's Republic of China"},{"name":"State Key Laboratory of Chirosciences, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheng","family":"Chen","sequence":"additional","affiliation":[{"name":"Shenzhen Key Laboratory for Food Biological Safety Control, Food Safety and Technology Research Centre, Hong Kong PolyU Shenzhen Research Institute, Shenzhen, People's Republic of China"},{"name":"State Key Laboratory of Chirosciences, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.9.3332-3337.2004"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.plasmid.2008.03.003"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkm167"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01574-08"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01144-12"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00139-10"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dks086"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dku340"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00725-13"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dks377"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/mst197"},{"key":"e_1_3_2_13_2","volume-title":"Performance standards for antimicrobial susceptibility testing; 23rd informational supplement","author":"Clinical and Laboratory Standards Institute","year":"2013","unstructured":"Clinical and Laboratory Standards Institute. 2013. Performance standards for antimicrobial susceptibility testing; 23rd informational supplement. CLSI M100-S23. Clinical and Laboratory Standards Institute, Wayne, PA."},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.06191-11"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.1999.tb04612.x"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00456-12"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2013.00125"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.00310-15","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.00310-15","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T23:07:22Z","timestamp":1645484842000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.00310-15"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,6]]},"references-count":16,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2015,6]]}},"alternative-id":["10.1128\/AAC.00310-15"],"URL":"https:\/\/doi.org\/10.1128\/aac.00310-15","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,6]]},"assertion":[{"value":"2015-02-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-03-16","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-05-14","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}