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Mosquitoes are vectors of multiple pathogens and have developed resistance mechanisms against them, which often involve antimicrobial peptides (AMPs). An-cecB is an AMP of the malaria-transmitting mosquito genus\n              <jats:italic>Anopheles<\/jats:italic>\n              , and we herein report its antimalarial activity against\n              <jats:italic>Plasmodium falciparum<\/jats:italic>\n              3D7, the artemisinin-resistant strain 803, and the chloroquine-resistant strain Dd2\n              <jats:italic>in vitro<\/jats:italic>\n              . We also demonstrate its anti-parasite activity\n              <jats:italic>in vivo<\/jats:italic>\n              , using the rodent malaria parasite\n              <jats:italic>Plasmodium berghei<\/jats:italic>\n              (ANKA). We show that An-cecB displays potent antimalarial activity and that its mechanism of action may occur through direct killing of the parasite or through interaction with infected red blood cell membranes. Unfortunately, An-cecB was found to be cytotoxic to mammalian cells and had poor antimalarial activity\n              <jats:italic>in vivo<\/jats:italic>\n              . However, its truncated peptide An-cecB-1 retained most of its antimalarial activity and avoided its cytotoxicity\n              <jats:italic>in vitro<\/jats:italic>\n              . An-cecB-1 also showed better antimalarial activity\n              <jats:italic>in vivo<\/jats:italic>\n              . Mosquito-derived AMPs may provide new ideas for the development of antimalarial drugs against drug-resistant parasites, and An-cecB has potential use as a template for antimalarial peptides.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/aac.00311-24","type":"journal-article","created":{"date-parts":[[2024,6,14]],"date-time":"2024-06-14T12:33:25Z","timestamp":1718368405000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":15,"title":["Antimalarial activity of cecropin antimicrobial peptides derived from\n            <i>Anopheles<\/i>\n            mosquitoes"],"prefix":"10.1128","volume":"68","author":[{"given":"Junchao","family":"Lou","sequence":"first","affiliation":[{"name":"School of Public Health, Nanjing Medical University, Nanjing, Jiangsu, China"},{"name":"National Health Commission Key Laboratory of Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and 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China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoding","family":"Zhu","sequence":"additional","affiliation":[{"name":"School of Public Health, Nanjing Medical University, Nanjing, Jiangsu, China"},{"name":"National Health Commission Key Laboratory of Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases, Wuxi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meihua","family":"Zhang","sequence":"additional","affiliation":[{"name":"National Health Commission Key Laboratory of Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases, Wuxi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianxia","family":"Tang","sequence":"additional","affiliation":[{"name":"School of Public Health, Nanjing Medical University, Nanjing, Jiangsu, China"},{"name":"National Health Commission Key Laboratory of Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases, Wuxi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaqun","family":"Fang","sequence":"additional","affiliation":[{"name":"Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9840-5238","authenticated-orcid":false,"given":"Xiaoqin","family":"He","sequence":"additional","affiliation":[{"name":"School of Public Health, Nanjing Medical University, Nanjing, Jiangsu, China"},{"name":"National Health Commission Key Laboratory of Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases, Wuxi, 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