{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T21:52:51Z","timestamp":1789854771196,"version":"4.0.1"},"reference-count":33,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2010,9,1]],"date-time":"2010-09-01T00:00:00Z","timestamp":1283299200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2010,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Identification of clinical isolates of\n            <jats:italic>Neisseria meningitidis<\/jats:italic>\n            that are resistant to rifampin is important to avoid prophylaxis failure in contacts of patients, but it is hindered by the absence of a breakpoint for resistance, despite many efforts toward standardization. We examined a large number (\n            <jats:italic>n<\/jats:italic>\n            = 392) of clinical meningococcal isolates, spanning 25 years (1984 to 2009), that were collected in 11 European countries, Argentina, and the Central African Republic. The collection comprises all clinical isolates with MICs of \u22650.25 mg\/liter (\n            <jats:italic>n<\/jats:italic>\n            = 161) received by the national reference laboratories for meningococci in the participating countries. Representative isolates displaying rifampin MICs of &lt;0.25 mg\/liter were also examined (\n            <jats:italic>n<\/jats:italic>\n            = 231). Typing of isolates was performed, and a 660-bp DNA fragment of the\n            <jats:italic>rpoB<\/jats:italic>\n            gene was sequenced. Sequences differing by at least one nucleotide were defined as unique\n            <jats:italic>rpoB<\/jats:italic>\n            alleles. The geometric mean of the MICs was calculated for isolates displaying the same allele. The clinical isolates displaying rifampin MICs of &gt;1 mg\/liter possessed\n            <jats:italic>rpoB<\/jats:italic>\n            alleles with nonsynonymous mutations at four critical amino acid residues, D542, H552, S548, and S557, that were absent in the alleles found in all isolates with MICs of \u22641 mg\/liter. Rifampin-susceptible isolates could be defined as those with MICs of \u22641 mg\/liter. The\n            <jats:italic>rpoB<\/jats:italic>\n            allele sequence and isolate data have been incorporated into the PubMLST\n            <jats:italic>Neisseria<\/jats:italic>\n            database (\n            <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"url\" xlink:href=\"http:\/\/pubmlst.org\/neisseria\/\">http:\/\/pubmlst.org\/neisseria\/<\/jats:ext-link>\n            ). The rifampin-resistant isolates belonged to diverse genetic lineages and were associated with lower levels of bacteremia and inflammatory cytokines in mice. This biological cost may explain the lack of clonal expansion of these isolates.\n          <\/jats:p>","DOI":"10.1128\/aac.00315-10","type":"journal-article","created":{"date-parts":[[2010,7,7]],"date-time":"2010-07-07T02:37:20Z","timestamp":1278470240000},"page":"3651-3658","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":41,"title":["Multicenter Study for Defining the Breakpoint for Rifampin Resistance in\n            <i>Neisseria meningitidis<\/i>\n            by\n            <i>rpoB<\/i>\n            Sequencing"],"prefix":"10.1128","volume":"54","author":[{"given":"Muhamed-Kheir","family":"Taha","sequence":"first","affiliation":[{"name":"Institut Pasteur, National Reference Centre for Meningococci, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Thulin Hedberg","sequence":"additional","affiliation":[{"name":"National Reference Laboratory for Pathogenic Neisseria, O\u0308rebro University Hospital, O\u0308rebro, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marek","family":"Szatanik","sequence":"additional","affiliation":[{"name":"Institut Pasteur, National Reference Centre for Meningococci, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Hong","sequence":"additional","affiliation":[{"name":"Institut Pasteur, National Reference Centre for Meningococci, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Corinne","family":"Ruckly","sequence":"additional","affiliation":[{"name":"Institut Pasteur, National Reference Centre for Meningococci, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raquel","family":"Abad","sequence":"additional","affiliation":[{"name":"Reference Laboratory for Neisserias, National Center for Microbiology, Institute of Health Carlos III, Majadahonda, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie","family":"Bertrand","sequence":"additional","affiliation":[{"name":"National Reference Centre for Neisseria meningitidis, Bacteriology Division, Scientific Institute of Public Health, Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francoise","family":"Carion","sequence":"additional","affiliation":[{"name":"National Reference Centre for Neisseria meningitidis, Bacteriology Division, Scientific Institute of Public Health, Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heike","family":"Claus","sequence":"additional","affiliation":[{"name":"Institute for Hygiene and Microbiology, National Reference Center for Meningococci, University of Wu\u0308rzburg, Wu\u0308rzburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandra","family":"Corso","sequence":"additional","affiliation":[{"name":"Depto de Bacterilogia INEI-ANLIS Dr. Carlos G. Malbran, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roci\u0301o","family":"Enri\u0301quez","sequence":"additional","affiliation":[{"name":"Reference Laboratory for Neisserias, National Center for Microbiology, Institute of Health Carlos III, Majadahonda, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sigrid","family":"Heuberger","sequence":"additional","affiliation":[{"name":"National Reference Centre for Meningococci, Austrian Agency for Health and Food Safety, Graz, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Waleria","family":"Hryniewicz","sequence":"additional","affiliation":[{"name":"National Reference Centre for Bacterial Meningitis, National Medicines Institute, Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keith A.","family":"Jolley","sequence":"additional","affiliation":[{"name":"Department of Zoology, University of Oxford, Oxford, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula","family":"Kriz","sequence":"additional","affiliation":[{"name":"National Reference Laboratory for Meningococcal Infections, NIPH, Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Mollerach","sequence":"additional","affiliation":[{"name":"Catedra de Microbiologia Facultad de Farmacia y Bioquimica, Universidad de Buenos Aires, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Musilek","sequence":"additional","affiliation":[{"name":"National Reference Laboratory for Meningococcal Infections, NIPH, Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arianna","family":"Neri","sequence":"additional","affiliation":[{"name":"Department of Infectious Parasitic and Immune-Mediated Diseases, Instituto Superiore di Sanita\u0300, Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Per","family":"Olce\u0301n","sequence":"additional","affiliation":[{"name":"National Reference Laboratory for Pathogenic Neisseria, O\u0308rebro University Hospital, O\u0308rebro, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"Pana","sequence":"additional","affiliation":[{"name":"Cantacuzino Institute, National Reference Center for Neisseria meningitidis, Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Skoczynska","sequence":"additional","affiliation":[{"name":"National Reference Centre for Bacterial Meningitis, National Medicines Institute, Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cecilia","family":"Sorhouet Pereira","sequence":"additional","affiliation":[{"name":"Depto de Bacterilogia INEI-ANLIS Dr. Carlos G. Malbran, Buenos Aires, Argentina"},{"name":"Catedra de Microbiologia Facultad de Farmacia y Bioquimica, Universidad de Buenos Aires, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Stefanelli","sequence":"additional","affiliation":[{"name":"Department of Infectious Parasitic and Immune-Mediated Diseases, Instituto Superiore di Sanita\u0300, Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georgina","family":"Tzanakaki","sequence":"additional","affiliation":[{"name":"National Meningitis Reference Laboratory, National School of Public Health, Athens, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magnus","family":"Unemo","sequence":"additional","affiliation":[{"name":"National Reference Laboratory for Pathogenic Neisseria, O\u0308rebro University Hospital, O\u0308rebro, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julio A.","family":"Va\u0301zquez","sequence":"additional","affiliation":[{"name":"Reference Laboratory for Neisserias, National Center for Microbiology, Institute of Health Carlos III, Majadahonda, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrich","family":"Vogel","sequence":"additional","affiliation":[{"name":"Institute for Hygiene and Microbiology, National Reference Center for Meningococci, University of Wu\u0308rzburg, Wu\u0308rzburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Izabela","family":"Wasko","sequence":"additional","affiliation":[{"name":"National Reference Centre for Bacterial Meningitis, National Medicines Institute, Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0882-4010(88)90045-9"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/47.3.285"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2005.07.014"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2007.00905.x"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2006.01617.x"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.38.6.1256"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.9.3066-3071.2001"},{"key":"e_1_3_2_9_2","unstructured":"Performance standards for antimicrobial susceptibility testing; 20th informational supplement. 2010"},{"key":"e_1_3_2_10_2","first-page":"515","volume":"4","year":"2002","unstructured":"Diggle, M. 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