{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T05:12:51Z","timestamp":1791090771662,"version":"4.1.0"},"reference-count":37,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2011,12,1]],"date-time":"2011-12-01T00:00:00Z","timestamp":1322697600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2011,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            KJ09C, a multidrug-resistant mutant of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Stenotrophomonas maltophilia<\/jats:named-content>\n            KJ, was generated by\n            <jats:italic>in vitro<\/jats:italic>\n            selection with chloramphenicol. The multidrug-resistant phenotype of KJ09C was attributed to overexpression of a resistance nodulation division (RND)-type efflux system encoded by an operon consisting of five genes:\n            <jats:italic>smeU1<\/jats:italic>\n            ,\n            <jats:italic>smeV<\/jats:italic>\n            ,\n            <jats:italic>smeW<\/jats:italic>\n            ,\n            <jats:italic>smeU2<\/jats:italic>\n            , and\n            <jats:italic>smeX<\/jats:italic>\n            . Proteins encoded by\n            <jats:italic>smeV<\/jats:italic>\n            ,\n            <jats:italic>smeW<\/jats:italic>\n            , and\n            <jats:italic>smeX<\/jats:italic>\n            were similar to the membrane fusion protein, RND transporter, and outer membrane protein, respectively, of known RND-type systems. The proteins encoded by\n            <jats:italic>smeU1<\/jats:italic>\n            and\n            <jats:italic>smeU2<\/jats:italic>\n            were found to belong to the family of short-chain dehydrogenases\/reductases. Mutant KJ09C exhibited increased resistance to chloramphenicol, quinolones, and tetracyclines and susceptibility to aminoglycosides; susceptibility to \u03b2-lactams and erythromycin was not affected. The expression of the\n            <jats:italic>smeU1-V-W-U2-X<\/jats:italic>\n            operon was regulated by the divergently transcribed LysR-type regulator gene\n            <jats:italic>smeRv<\/jats:italic>\n            . Overexpression of the SmeVWX pump contributed to the acquired resistance to chloramphenicol, quinolones, and tetracyclines. Inactivation of\n            <jats:italic>smeV<\/jats:italic>\n            and\n            <jats:italic>smeW<\/jats:italic>\n            completely abolished the activity of the SmeVWX pump, whereas inactivation of\n            <jats:italic>smeX<\/jats:italic>\n            alone decreased the activity of the SmeVWX pump. The enhanced aminoglycoside susceptibility observed in KJ09C resulted from SmeX overexpression.\n          <\/jats:p>","DOI":"10.1128\/aac.00317-11","type":"journal-article","created":{"date-parts":[[2011,9,20]],"date-time":"2011-09-20T08:18:33Z","timestamp":1316506713000},"page":"5826-5833","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":81,"title":["Contribution of Resistance-Nodulation-Division Efflux Pump Operon\n            <i>smeU1-V-W-U2-X<\/i>\n            to Multidrug Resistance of Stenotrophomonas maltophilia"],"prefix":"10.1128","volume":"55","author":[{"given":"Chao-Hsien","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung 404, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiang-Ching","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung 404, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsao-Chuen","family":"Chung","sequence":"additional","affiliation":[{"name":"School of Medical Laboratory Science and Biotechnology, Taipei Medical University, Taipei 110, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rouh-Mei","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Asia University, Taichung 413, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Wei","family":"Huang","sequence":"additional","affiliation":[{"name":"Graduate Institute of Veterinary Public Health, National Chung-Hsing University, Taichung 402, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsuey-Ching","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung 404, Taiwan"},{"name":"Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, Taipei 112, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.03.004"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.11.3079-3086.2000"},{"key":"e_1_3_2_4_2","volume-title":"M100-S20.","author":"Clinical and Laboratory Standards Institute","year":"2010","unstructured":"Clinical and Laboratory Standards Institute. 2010. 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