{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T05:25:55Z","timestamp":1791091555019,"version":"4.1.0"},"reference-count":54,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2013,9,1]],"date-time":"2013-09-01T00:00:00Z","timestamp":1377993600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2013,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Kingella kingae<\/jats:named-content>\n            is a human pathogen that causes pediatric osteoarticular infections and infective endocarditis in children and adults. The bacterium is usually susceptible to \u03b2-lactam antibiotics, although \u03b2-lactam resistance has been reported in rare isolates. This study was conducted to identify \u03b2-lactam-resistant strains and to characterize the resistance mechanism. Screening of a set of 90\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">K. kingae<\/jats:named-content>\n            clinical isolates obtained from different geographic locations revealed high-level resistance to penicillins among 25% of the strains isolated from Minnesota and Iceland. These strains produced TEM-1 \u03b2-lactamase and were shown to contain additional \u226550-kb plasmids. Ion Torrent sequencing of extrachromosomal DNA from a \u03b2-lactamase-producing strain confirmed the plasmid location of the\n            <jats:italic>\n              bla\n              <jats:sub>TEM<\/jats:sub>\n            <\/jats:italic>\n            gene. An identical plasmid pattern was demonstrated by multiplex PCR in all \u03b2-lactamase producers. The porin gene's fragments were analyzed to investigate the relatedness of bacterial strains. Phylogenetic analysis revealed 27 single-nucleotide polymorphisms (SNPs) in the\n            <jats:italic>por<\/jats:italic>\n            gene fragment, resulting in two major clusters with 11 allele types forming bacterial-strain subclusters. \u03b2-Lactamase producers were grouped together based on\n            <jats:italic>por<\/jats:italic>\n            genotyping. Our results suggest that the \u03b2-lactamase-producing strains likely originate from a single plasmid-bearing\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">K. kingae<\/jats:named-content>\n            isolate that traveled from Europe to the United States, or vice versa. This study highlights the prevalence of penicillin resistance among\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">K. kingae<\/jats:named-content>\n            strains in some regions and emphasizes the importance of surveillance for antibiotic resistance of the pathogen.\n          <\/jats:p>","DOI":"10.1128\/aac.00318-13","type":"journal-article","created":{"date-parts":[[2013,6,25]],"date-time":"2013-06-25T02:01:36Z","timestamp":1372125696000},"page":"4300-4306","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":20,"title":["Characterization of TEM-1 \u03b2-Lactamase-Producing Kingella kingae Clinical Isolates"],"prefix":"10.1128","volume":"57","author":[{"given":"Anushree","family":"Banerjee","sequence":"first","affiliation":[{"name":"Department of Oral Biology, New Jersey Dental School, Newark, New Jersey, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey B.","family":"Kaplan","sequence":"additional","affiliation":[{"name":"Department of Biology, American University, Washington, DC, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amenah","family":"Soherwardy","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, New Jersey Dental School, Newark, New Jersey, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoav","family":"Nudell","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, New Jersey Dental School, Newark, New Jersey, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grace A.","family":"Mackenzie","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, New Jersey Dental School, Newark, New Jersey, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shannon","family":"Johnson","sequence":"additional","affiliation":[{"name":"Genome Biology Group, Los Alamos National Laboratory, Los Alamos, New Mexico, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nataliya V.","family":"Balashova","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, New Jersey Dental School, Newark, New Jersey, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11832-009-0233-2"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.inf.0000259954.88139.f4"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00144-09"},{"key":"e_1_3_3_5_2","first-page":"251","article-title":"Kingella kingae prosthetic valve endocarditis complicated by a paravalvular abscess","volume":"11","author":"Korach A","year":"2009","unstructured":"KorachAOlshtain-PopsKSchwartzDMosesA. 2009. Kingella kingae prosthetic valve endocarditis complicated by a paravalvular abscess. Isr. Med. Assoc. J. 11:251\u2013253.","journal-title":"Isr. Med. Assoc. J."},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.inf.0000083848.93457.e7"},{"key":"e_1_3_3_7_2","first-page":"353","article-title":"Mitral acute bacterial endocarditis caused by HACEK microorganisms","volume":"18","author":"Nguyen S","year":"2009","unstructured":"NguyenSFayadGModineTLeroyO. 2009. Mitral acute bacterial endocarditis caused by HACEK microorganisms. J. Heart Valve Dis. 18:353\u2013354.","journal-title":"J. Heart Valve Dis."},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.inf.0000172153.10569.dc"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1097\/00006454-199508000-00005"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0163-4453(86)92841-0"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1001\/archneur.57.12.1774"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1097\/00006454-199109000-00011"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbspin.2005.10.021"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1177\/000992280404300311"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2010-1867"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.inf.0000215243.42501.4f"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1097\/INF.0b013e3181b48cc3"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.58.4.920-929.1990"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.106.183095"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-004-4060-9"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/iub.532"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/17.4.818"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/33.3.635"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.3109\/00365549709011861"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1097\/INF.0b013e31824041b8"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0038078"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cis622"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1097\/INF.0b013e318240cf8a"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2004-2051"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.74.4.2015-2021.2006"},{"key":"e_1_3_3_31_2","volume-title":"Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; approved standard","author":"Clinical and Laboratory Standards Institute","unstructured":"Clinical and Laboratory Standards Institute. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; approved standard. Document M07-A9, 9th ed, vol 32. Clinical and Laboratory Standards Institute, Wayne, PA.","edition":"9"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gnj007"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01690097"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.12.3724-3732.2003"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01319-06"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1006\/plas.1999.1431"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.188.7.2300-2308.2006"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/47.2.191"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.8.4.557"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dki281"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.1.210-212.2000"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01997057"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01009-09"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01509-06"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02353-09"},{"key":"e_1_3_3_46_2","first-page":"105","article-title":"Risk factors for extended-spectrum beta-lactamase-producing Escherichia coli infection in hospitalized patients","volume":"74","author":"Ikeda Y","year":"2012","unstructured":"IkedaYMamiyaTNishiyamaHKosekiTMouriANabeshimaT. 2012. Risk factors for extended-spectrum beta-lactamase-producing Escherichia coli infection in hospitalized patients. Nagoya J. Med. Sci. 74:105\u2013114.","journal-title":"Nagoya J. Med. Sci."},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00421-12"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01418-12"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268898008656"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.12.3680-3682.1998"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01082-07"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1086\/374563"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2007\/015479-0"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.52.35332"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2011.03.005"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.00318-13","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.00318-13","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T22:30:47Z","timestamp":1645482647000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.00318-13"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,9]]},"references-count":54,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2013,9]]}},"alternative-id":["10.1128\/AAC.00318-13"],"URL":"https:\/\/doi.org\/10.1128\/aac.00318-13","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,9]]},"assertion":[{"value":"2013-02-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-06-17","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-08-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}