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Current therapies for toxoplasmosis are restricted to tachyzoites and have little or no effect on bradyzoites, which are maintained in tissue cysts. Consequently, new therapeutic alternatives have been proposed as the use of atovaquone has demonstrated partial efficacy against tachyzoites and bradyzoites. This work studies the effect of 3-bromopyruvate (3-BrPA), a compound that is being tested against cancer cells, on the infection of LLC-MK2 cells with\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">T. gondii<\/jats:named-content>\n            tachyzoites, RH strain. No effect of 3-BrPA on host cell proliferation or viability was observed, but it inhibited the proliferation of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">T. gondii<\/jats:named-content>\n            . The incubation of cultures with lectin\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Dolichos biflorus<\/jats:named-content>\n            agglutinin (DBA) showed the development of cystogenesis, and an ultrastructural analysis of parasite intracellular development confirmed morphological characteristics commonly found in tissue cysts. Moreover, the presence of degraded parasites and the influence of 3-BrPA on endodyogeny were observed. Infected cultures were alternatively treated with a combination of this compound plus atovaquone. This resulted in a 73% reduction in intracellular parasites after 24 h of treatment and a 71% reduction after 48 h; cyst wall formation did not occur in these cultures. Therefore, we conclude that the use of 3-BrPA may serve as an important tool for the study of (i)\n            <jats:italic>in vitro<\/jats:italic>\n            cystogenesis; (ii) parasite metabolism, requiring a deeper understanding of the target of action of this compound on\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">T. gondii<\/jats:named-content>\n            ; (iii) the alternative parasite metabolic pathways; and (iv) the molecular\/cellular mechanisms that trigger parasite death.\n          <\/jats:p>","DOI":"10.1128\/aac.00337-15","type":"journal-article","created":{"date-parts":[[2015,6,16]],"date-time":"2015-06-16T03:01:14Z","timestamp":1434423674000},"page":"5239-5249","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":18,"title":["Effect of 3-Bromopyruvate and Atovaquone on Infection during\n            <i>In Vitro<\/i>\n            Interaction of Toxoplasma gondii and LLC-MK2 Cells"],"prefix":"10.1128","volume":"59","author":[{"given":"Loyze Paola O.","family":"de Lima","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Biologia Estrutural, Instituto Oswaldo Cruz, Fiocruz, Manguinhos, Rio de Janeiro, Brazil"},{"name":"Laborat\u00f3rio de Tecnologia em Bioqu\u00edmica e Microscopia, Centro Universit\u00e1rio Estadual da Zona Oeste (UEZO), Campo Grande, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio H.","family":"Seabra","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Tecnologia em Bioqu\u00edmica e Microscopia, Centro Universit\u00e1rio Estadual da Zona Oeste (UEZO), Campo Grande, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henrique","family":"Carneiro","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Estrutural, Instituto Oswaldo Cruz, Fiocruz, Manguinhos, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5672-5444","authenticated-orcid":false,"given":"Helene S.","family":"Barbosa","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Estrutural, Instituto Oswaldo Cruz, Fiocruz, Manguinhos, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(00)00124-7"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.64.3.607-623.2000"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)16412-X"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0104-42302003000100016"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.5.1163-1173.2002"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.40.9.2015"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01203-07"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.9.2240"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2004.09.047"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1158\/1535-7163.MCT-07-0115"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10863-012-9422-7"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3835(01)00667-X"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10863-012-9419-2"},{"key":"e_1_3_2_15_2","first-page":"356","article-title":"Safety and outcome of treatment of metastatic melanoma using 3-bromopyruvate: a concise literature review and case study","volume":"33","author":"El Sayed SM","year":"2014","unstructured":"El Sayed SM, Mohamed WG, Seddik MA, Ahmed AS, Mahmoud AG, Amer WH, Helmy Nabo MM, Hamed AR, Ahmed NS, Abd-Allah AA. 2014. 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