{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T03:03:05Z","timestamp":1790910185966,"version":"4.1.0"},"reference-count":26,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2006,10,1]],"date-time":"2006-10-01T00:00:00Z","timestamp":1159660800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2006,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            In recent decades, the prevalence of\n            <jats:italic>Neisseria meningitidis<\/jats:italic>\n            isolates with reduced susceptibility to penicillins has increased. The intermediate resistance to penicillin (Pen\n            <jats:sup>i<\/jats:sup>\n            ) for most strains is due mainly to mosaic structures in the\n            <jats:italic>penA<\/jats:italic>\n            gene, encoding penicillin-binding protein 2. In this study, susceptibility to \u03b2-lactam antibiotics was determined for 60 Swedish clinical\n            <jats:italic>N. meningitidis<\/jats:italic>\n            isolates and 19 reference strains. The\n            <jats:italic>penA<\/jats:italic>\n            gene was sequenced and compared to 237\n            <jats:italic>penA<\/jats:italic>\n            sequences from GenBank in order to explore the total identified variation of\n            <jats:italic>penA<\/jats:italic>\n            . The divergent mosaic alleles differed by 3% to 24% compared to those of the designated wild-type\n            <jats:italic>penA<\/jats:italic>\n            gene. By studying the final 1,143 to 1,149 bp of\n            <jats:italic>penA<\/jats:italic>\n            in a sequence alignment, 130 sequence variants were identified. In a 402-bp alignment of the most variable regions, 84 variants were recognized. Good correlation between elevated MICs and the presence of\n            <jats:italic>penA<\/jats:italic>\n            mosaic structures was found especially for penicillin G and ampicillin. The Pen\n            <jats:sup>i<\/jats:sup>\n            isolates comprised an MIC of &gt;0.094 \u03bcg\/ml for penicillin G and an MIC of &gt;0.064 \u03bcg\/ml for ampicillin. Ampicillin was the best antibiotic for precise categorization as Pen\n            <jats:sup>s<\/jats:sup>\n            or Pen\n            <jats:sup>i<\/jats:sup>\n            . In comparison with the wild-type\n            <jats:italic>penA<\/jats:italic>\n            sequence, two specific Pen\n            <jats:sup>i<\/jats:sup>\n            sites were altered in all except two mosaic\n            <jats:italic>penA<\/jats:italic>\n            sequences, which were published in GenBank and no MICs of the corresponding isolates were described. In conclusion, monitoring the relationship between\n            <jats:italic>penA<\/jats:italic>\n            sequences and MICs to penicillins is crucial for developing fast and objective methods for susceptibility determination. By studying the\n            <jats:italic>penA<\/jats:italic>\n            gene, genotypical determination of susceptibility in culture-negative cases can also be accomplished.\n          <\/jats:p>","DOI":"10.1128\/aac.00353-06","type":"journal-article","created":{"date-parts":[[2006,9,27]],"date-time":"2006-09-27T17:13:29Z","timestamp":1159377209000},"page":"3317-3324","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":37,"title":["Total Variation in the\n            <i>penA<\/i>\n            Gene of\n            <i>Neisseria meningitidis<\/i>\n            : Correlation between Susceptibility to \u03b2-Lactam Antibiotics and\n            <i>penA<\/i>\n            Gene Heterogeneity"],"prefix":"10.1128","volume":"50","author":[{"given":"Sara","family":"Thulin","sequence":"first","affiliation":[{"name":"National Reference Laboratory for Pathogenic Neisseria, Department of Clinical Microbiology, O\u0308rebro University Hospital, O\u0308rebro, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Per","family":"Olce\u0301n","sequence":"additional","affiliation":[{"name":"National Reference Laboratory for Pathogenic Neisseria, Department of Clinical Microbiology, O\u0308rebro University Hospital, O\u0308rebro, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans","family":"Fredlund","sequence":"additional","affiliation":[{"name":"National Reference Laboratory for Pathogenic Neisseria, Department of Clinical Microbiology, O\u0308rebro University Hospital, O\u0308rebro, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magnus","family":"Unemo","sequence":"additional","affiliation":[{"name":"National Reference Laboratory for Pathogenic Neisseria, Department of Clinical Microbiology, O\u0308rebro University Hospital, O\u0308rebro, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.12.3744-3749.2002"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M304607200"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/47.3.285"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.1.358-359.2004"},{"key":"e_1_3_2_6_2","first-page":"107","year":"2001","unstructured":"Arreaza, L., and J. A. V\u00e1zquez. 2001. Molecular approach for the study of penicillin resistance in Neisseria meningitidis, p. 107-119. In A. J. Pollard and M. C. J. Maiden (ed.), Meningococcal disease: methods and protocols. Humana Press, Totowa, N.J.","journal-title":"Meningococcal disease: methods and protocols"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.176.2.333-337.1994"},{"key":"e_1_3_2_8_2","unstructured":"Clinical and Laboratory Standards Institute. 2005. Performance standards for antimicrobial susceptibility testing; 15th informational supplement. CLSI\/NCCLS M100-S15. Clinical and Laboratory Standards Institute Wayne Pa."},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.31.12.3255-3259.1993"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.7.3162-3171.2005"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.12.4531-4535.2002"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.34.7.1637-1640.1996"},{"key":"e_1_3_2_13_2","unstructured":"Olc\u00e9n P. H. Fredlund P. M\u00f6lling and M. Unemo. 2006. Annual report concerning serogroup serotype genosubtype and antibiotic sensitivity for Swedish Neisseria meningitidis isolates and results with direct PCR for diagnosis of acute bacterial meningitis 2005. National Reference Laboratory for Pathogenic Neisseria \u00d6rebro Sweden."},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.3109\/00365549509047063"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/24.1.98"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0924-8579(01)00362-4"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/5.1.71"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199703063361007"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.3.769-777.2002"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.22.8988"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.8153626"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.10.4666-4670.2003"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.50.3.887-892.2006"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.287.5459.1809"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.9.4141-4147.2003"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268898008656"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.11.3430-3434.2003"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.00353-06","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.00353-06","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T18:40:21Z","timestamp":1645468821000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.00353-06"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,10]]},"references-count":26,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2006,10]]}},"alternative-id":["10.1128\/AAC.00353-06"],"URL":"https:\/\/doi.org\/10.1128\/aac.00353-06","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,10]]},"assertion":[{"value":"2006-03-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2006-07-20","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2006-10-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}