{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T02:45:52Z","timestamp":1774406752803,"version":"3.50.1"},"reference-count":25,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2008,2,1]],"date-time":"2008-02-01T00:00:00Z","timestamp":1201824000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2008,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            In\n            <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n            , it is common for plasmid-located and chromosome-located\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>SHV<\/jats:sub>\n            copies to coexist within single cells. The plasmid-borne genes are mainly derived from two separate IS\n            <jats:italic>26<\/jats:italic>\n            -mediated mobilizations of\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>SHV<\/jats:sub>\n            . The objective of this study was to test the hypothesis that the presence of a non-extended-spectrum \u03b2-lactamase (non-ESBL) encoding plasmid-borne form of\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>SHV<\/jats:sub>\n            facilitates the cefotaxime (CTX)-mediated selection of ESBL-expressing mutants, even when there is a chromosomal copy of the same gene. Twenty-one diverse ESBL-negative,\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>TEM<\/jats:sub>\n            -negative\n            <jats:italic>K. pneumoniae<\/jats:italic>\n            clinical isolates were tested for the IS\n            <jats:italic>26<\/jats:italic>\n            insertions characteristic of the two mobilization events. The isolates were then tested for their ability to be selected for ESBL-mediated CTX resistance by serial subculturing with a doubling of the CTX concentration at every subculture. Fourteen isolates possessed neither of the IS\n            <jats:italic>26<\/jats:italic>\n            insertions. None of these became ESBL positive, and all died during the course of the experiment, despite possessing chromosomal\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>SHV<\/jats:sub>\n            copies. The other isolates all became ESBL positive and grew abundantly up to a CTX concentration of 128 \u03bcg\/ml. Similar results were obtained with ceftazidime. ESBL expression was associated with the appearance of the expected G\u2192A mutation at position 1 of codon 238 and also with\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>SHV<\/jats:sub>\n            copy number amplification. It was concluded that plasmid-borne\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>SHV<\/jats:sub>\n            greatly facilitates the selection of ESBL expression, even when the same gene is on the chromosome, and that gene dosage effects are likely to contribute to this phenomenon.\n          <\/jats:p>","DOI":"10.1128\/aac.00359-07","type":"journal-article","created":{"date-parts":[[2007,11,14]],"date-time":"2007-11-14T01:23:33Z","timestamp":1195003413000},"page":"441-445","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":19,"title":["Selection of SHV Extended-Spectrum-\u03b2-Lactamase-Dependent Cefotaxime and Ceftazidime Resistance in\n            <i>Klebsiella pneumoniae<\/i>\n            Requires a Plasmid-Borne\n            <i>bla<\/i>\n            <sub>SHV<\/sub>\n            Gene"],"prefix":"10.1128","volume":"52","author":[{"given":"David S.","family":"Hammond","sequence":"first","affiliation":[{"name":"Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Queensland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tegan","family":"Harris","sequence":"additional","affiliation":[{"name":"Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Queensland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Bell","sequence":"additional","affiliation":[{"name":"Women's and Children's Hospital, Adelaide, South Australia, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Turnidge","sequence":"additional","affiliation":[{"name":"Women's and Children's Hospital, Adelaide, South Australia, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philip M.","family":"Giffard","sequence":"additional","affiliation":[{"name":"Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Queensland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","unstructured":"Applied Biosystems 2000"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.8.2230-2230.2000"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.14.4.933-951.2001"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.10.2856-2861.2001"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.72.5.1720"},{"key":"e_1_3_2_7_2","unstructured":"Performance standards for antimicrobial susceptibility testing: 17th informational supplement M100-S17. 2007"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2006.07.004"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkh251"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1198-743x.2001.00330.x"},{"key":"e_1_3_2_11_2","first-page":"951","volume":"33","year":"1995","unstructured":"Gutmann, L., B. 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French. 1997. Mechanism and stability of hyperproduction of the extended spectrum \u03b2-lactamase SHV-5 in Klebsiella pneumoniae. J. Antimicrob. Chemother.40:525-532.","journal-title":"J. Antimicrob. 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