{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T12:57:07Z","timestamp":1790773027974,"version":"4.1.0"},"reference-count":50,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2015,7,1]],"date-time":"2015-07-01T00:00:00Z","timestamp":1435708800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2015,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The resistance-nodulation-division (RND)-type efflux pump is one of the causes of the multidrug resistance of\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Stenotrophomonas maltophilia<\/jats:named-content>\n                    . The roles of the RND-type efflux pump in physiological functions and virulence, in addition to antibiotic extrusion, have attracted much attention. In this study, the contributions of the constitutively expressed SmeYZ efflux pump to drug resistance, virulence-related characteristics, and virulence were evaluated.\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">S. maltophilia<\/jats:named-content>\n                    KJ is a clinical isolate of multidrug resistance. The\n                    <jats:italic>smeYZ<\/jats:italic>\n                    isogenic deletion mutant, KJ\u0394YZ, was constructed by a gene replacement strategy. The antimicrobial susceptibility, virulence-related physiological characteristics, susceptibility to human serum and neutrophils, and\n                    <jats:italic>in vivo<\/jats:italic>\n                    virulence between KJ and KJ\u0394YZ were comparatively assessed. The SmeYZ efflux pump contributed resistance to aminoglycosides and trimethoprim-sulfamethoxazole. Inactivation of\n                    <jats:italic>smeYZ<\/jats:italic>\n                    resulted in attenuation of oxidative stress susceptibility, swimming, flagella formation, biofilm formation, and secreted protease activity. Furthermore, loss of SmeYZ increased susceptibility to human serum and neutrophils and decreased\n                    <jats:italic>in vivo<\/jats:italic>\n                    virulence in a murine model. These findings suggest the possibility of attenuation of the resistance and virulence of\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">S. maltophilia<\/jats:named-content>\n                    with inhibitors of the SmeYZ efflux pump.\n                  <\/jats:p>","DOI":"10.1128\/aac.00372-15","type":"journal-article","created":{"date-parts":[[2015,4,27]],"date-time":"2015-04-27T21:51:47Z","timestamp":1430171507000},"page":"4067-4073","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":96,"title":["The SmeYZ Efflux Pump of Stenotrophomonas maltophilia Contributes to Drug Resistance, Virulence-Related Characteristics, and Virulence in Mice"],"prefix":"10.1128","volume":"59","author":[{"given":"Yi-Tsung","family":"Lin","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, Taipei Veterans General Hospital, Taipei, Taiwan"},{"name":"School of Medicine, National Yang-Ming University, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Wei","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiang-Jiuun","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Life Science, Institute of Ecology and Evolutionary Biology and TechComm-5, College of Life Science, National Taiwan University, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chia-Wei","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsuey-Ching","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.2217\/fmb.09.45"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00019-11"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.1997.tb00345.x"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2009.02.017"},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1128\/microbe.3.179.1","article-title":"Bacteria multidrug efflux pumps serve other functions","volume":"3","author":"Poole K","year":"2008","unstructured":"Poole K. 2008. 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