{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T06:06:08Z","timestamp":1791093968628,"version":"4.1.0"},"reference-count":32,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2007,10,1]],"date-time":"2007-10-01T00:00:00Z","timestamp":1191196800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2007,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Initial attachment of the cariogenic\n            <jats:italic>Streptococcus mutans<\/jats:italic>\n            onto dental enamel is largely promoted by the adsorption of specific salivary proteins on enamel surface. Some phosphorylated salivary proteins were found to reduce\n            <jats:italic>S. mutans<\/jats:italic>\n            adhesion by competitively inhibiting the adsorption of\n            <jats:italic>S. mutans<\/jats:italic>\n            -binding salivary glycoproteins to hydroxyapatite (HA). The aim of this study was to develop antiadherence compounds for preventing dental biofilm development. We synthesized phosphorylated polyethylene glycol (PEG) derivatives and examined the possibility of surface pretreatment with them for preventing\n            <jats:italic>S. mutans<\/jats:italic>\n            adhesion in vitro and dental biofilm formation in vivo. Pretreatment of the HA surface with methacryloyloxydecyl phosphate (MDP)-PEG prior to saliva incubation hydrophilized the surface and thereby reduced salivary protein adsorption and saliva-promoted bacterial attachment to HA. However, when MDP-PEG was added to the saliva-pretreated HA (S-HA) surface, its inhibitory effect on bacterial binding was completely diminished.\n            <jats:italic>S. mutans<\/jats:italic>\n            adhesion onto S-HA was successfully reduced by treatment of the surface with pyrophosphate (PP), which desorbs salivary components from S-HA. Treatment of S-HA surfaces with MDP-PEG plus PP completely inhibited saliva-promoted\n            <jats:italic>S. mutans<\/jats:italic>\n            adhesion even when followed by additional saliva treatment. Finally, mouthwash with MDP-PEG plus PP prevented de novo biofilm development after thorough teeth cleaning in humans compared to either water or PP alone. We conclude that MDP-PEG plus PP has the potential for use as an antiadherence agent that prevents dental biofilm development.\n          <\/jats:p>","DOI":"10.1128\/aac.00380-07","type":"journal-article","created":{"date-parts":[[2007,7,24]],"date-time":"2007-07-24T00:59:18Z","timestamp":1185238758000},"page":"3634-3641","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":30,"title":["Reduction of\n            <i>Streptococcus mutans<\/i>\n            Adherence and Dental Biofilm Formation by Surface Treatment with Phosphorylated Polyethylene Glycol"],"prefix":"10.1128","volume":"51","author":[{"given":"Akira","family":"Shimotoyodome","sequence":"first","affiliation":[{"name":"Biological Science Laboratories, Kao Corporation, Tochigi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Koudate","sequence":"additional","affiliation":[{"name":"Materials Development Research Laboratories, Kao Corporation, Wakayama, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hisataka","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Safety and Microbial Control Research Center, Kao Corporation, Tochigi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junji","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Biological Science Laboratories, Kao Corporation, Tochigi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ichiro","family":"Tokimitsu","sequence":"additional","affiliation":[{"name":"Health Care Products Research Laboratories No. 1, Kao Corporation, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tadashi","family":"Hase","sequence":"additional","affiliation":[{"name":"Biological Science Laboratories, Kao Corporation, Tochigi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Inoue","sequence":"additional","affiliation":[{"name":"Department of Clinical Pathophysiology, Tokyo Dental College, Chiba, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Matsukubo","sequence":"additional","affiliation":[{"name":"Department of Epidemiology and Public Health, Tokyo Dental College, Chiba, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshinori","family":"Takaesu","sequence":"additional","affiliation":[{"name":"Department of Epidemiology and Public Health, Tokyo Dental College, Chiba, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(89)90070-8"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1177\/10454411980090010401"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.60.3.1008-1017.1992"},{"key":"e_1_3_2_5_2","first-page":"199","volume":"8","year":"1992","unstructured":"Busscher, H. 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