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HMW615, an appendiceal isolate, contains at least two copies of\n            <jats:italic>nimJ<\/jats:italic>\n            on its genome, while HMW616, an isolate from a patient with sepsis, contains one genomic copy of\n            <jats:italic>nimJ. B. fragilis<\/jats:italic>\n            NimJ is phylogenetically closer to\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Prevotella baroniae<\/jats:named-content>\n            NimI and\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Clostridium botulinum<\/jats:named-content>\n            NimA than to the other known\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Bacteroides<\/jats:named-content>\n            Nim proteins. The predicted protein structure of NimJ, based on fold recognition analysis, is consistent with the crystal structures derived for known Nim proteins, and specific amino acid residues important for substrate binding in the active site are conserved. This study demonstrates that the \u201cuniversal\u201d\n            <jats:italic>nim<\/jats:italic>\n            primers will not detect all\n            <jats:italic>nim<\/jats:italic>\n            genes with the ability to confer metronidazole resistance, but\n            <jats:italic>nimJ<\/jats:italic>\n            alone cannot account for the very high metronidazole MICs of these resistant clinical isolates.\n          <\/jats:p>","DOI":"10.1128\/aac.00386-13","type":"journal-article","created":{"date-parts":[[2013,5,29]],"date-time":"2013-05-29T04:58:59Z","timestamp":1369803539000},"page":"3767-3774","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":50,"title":["Two Multidrug-Resistant Clinical Isolates of Bacteroides fragilis Carry a Novel Metronidazole Resistance\n            <i>nim<\/i>\n            Gene (\n            <i>nimJ<\/i>\n            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