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This latent reservoir is capable of producing replication-competent infectious viruses once prolonged suppressive cART is withdrawn. Inducing the reactivation of HIV-1 gene expression in T-cells harboring a latent provirus in people living with HIV-1 under cART may result in depletion of this latent reservoir due to cytopathic effects or immune clearance. Studies have investigated molecules that reactivate HIV-1 gene expression, but to date, no latency reversal agent has been identified to eliminate latently infected cells harboring replication-competent HIV in cART-treated individuals. Stochastic fluctuations in HIV-1\n            <jats:italic>tat<\/jats:italic>\n            gene expression have been described and hypothesized to allow the progression into proviral latency. We hypothesized that exposing latently infected CD4+ T-cells to Tat would result in effective latency reversal. Our results indicate the capacity of a truncated Tat protein and mRNA to reactivate HIV-1 in latently infected T-cells\n            <jats:italic>ex vivo<\/jats:italic>\n            to a similar degree as the protein kinase C agonist: phorbol 12-myristate 13-acetate, without T-cell activation or any significant transcriptome perturbation.\n          <\/jats:p>","DOI":"10.1128\/aac.00417-23","type":"journal-article","created":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T14:31:12Z","timestamp":1698157872000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":14,"title":["A truncated HIV Tat demonstrates potent and specific latency reversal activity"],"prefix":"10.1128","volume":"67","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-3343-0644","authenticated-orcid":true,"given":"Ellen","family":"Van Gulck","sequence":"first","affiliation":[{"name":"Janssen Infectious Diseases, Janssen Research and Development, A 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