{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T06:21:27Z","timestamp":1778566887270,"version":"3.51.4"},"reference-count":19,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2018,7,1]],"date-time":"2018-07-01T00:00:00Z","timestamp":1530403200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["15-15-00072"],"award-info":[{"award-number":["15-15-00072"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001826","name":"ZonMw","doi-asserted-by":"publisher","award":["50-52200-98-313"],"award-info":[{"award-number":["50-52200-98-313"]}],"id":[{"id":"10.13039\/501100001826","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2018,7]]},"abstract":"ABSTRACT<\/jats:title>\n \n Borrelia miyamotoi<\/jats:named-content>\n is an emerging relapsing fever (RF)\n Borrelia<\/jats:named-content>\n species that is reported to cause human disease in regions in which Lyme borreliosis is endemic. We recently showed that\n B. miyamotoi<\/jats:named-content>\n tick isolates are resistant to amoxicillin\n in vitro<\/jats:italic>\n ; however, clinical isolates have not been studied. Therefore, our aim was to show the antimicrobial susceptibility of recently obtained clinical isolates of\n B. miyamotoi<\/jats:named-content>\n . A dilution series of various antibiotics was made in modified Kelly-Pettenkofer medium with 10% fetal calf serum. The susceptibilities of different\n B. miyamotoi<\/jats:named-content>\n clinical,\n B. miyamotoi<\/jats:named-content>\n tick, RF\n Borrelia<\/jats:named-content>\n , and\n Borrelia burgdorferi<\/jats:named-content>\n sensu lato<\/jats:named-content>\n isolates were tested by measuring MICs through colorimetric changes and by counting motile spirochetes by dark-field microscopy after 72 h of incubation. The ceftriaxone and azithromycin MIC ranges of the six\n B. miyamotoi<\/jats:named-content>\n clinical isolates tested were 0.03 to 0.06 mg\/liter and 0.0016 to 0.0032 mg\/liter, respectively. These values are similar to MICs for RF\n Borrelia<\/jats:named-content>\n strains and\n B. miyamotoi<\/jats:named-content>\n tick isolates. All tested RF\n Borrelia<\/jats:named-content>\n strains were susceptible to doxycycline (microscopic MIC range, 0.0625 to 0.25 mg\/liter). In contrast to the MICs of the tested\n B. burgdorferi sensu lato<\/jats:named-content>\n strains and in line with our previous findings, the amoxicillin MICs (range, 8 to 32 mg\/liter) of all RF\n Borrelia<\/jats:named-content>\n strains, including\n B. miyamotoi<\/jats:named-content>\n clinical isolates, were above the clinical breakpoint for resistance (\u22644 mg\/liter). Clinical isolates of\n B. miyamotoi<\/jats:named-content>\n are highly susceptible to doxycycline, azithromycin, and ceftriaxone\n in vitro<\/jats:italic>\n . Interestingly, as described previously for tick isolates, amoxicillin shows poor\n in vitro<\/jats:italic>\n activity against\n B. miyamotoi<\/jats:named-content>\n clinical isolates.\n <\/jats:p>","DOI":"10.1128\/aac.00419-18","type":"journal-article","created":{"date-parts":[[2018,4,17]],"date-time":"2018-04-17T09:53:01Z","timestamp":1523958781000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":12,"title":["In Vitro<\/i>\n Antimicrobial Susceptibility of Clinical Isolates of Borrelia miyamotoi"],"prefix":"10.1128","volume":"62","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4604-5111","authenticated-orcid":false,"given":"Joris","family":"Koetsveld","sequence":"first","affiliation":[{"name":"Center for Experimental and Molecular Medicine, Academic Medical Center, University of Amsterdam, Amsterdam, the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annemijn","family":"Manger","sequence":"additional","affiliation":[{"name":"Center for Experimental and Molecular Medicine, Academic Medical Center, University of Amsterdam, Amsterdam, the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dieuwertje","family":"Hoornstra","sequence":"additional","affiliation":[{"name":"Center for Experimental and Molecular Medicine, Academic Medical Center, University of Amsterdam, Amsterdam, the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ronald O.","family":"Draga","sequence":"additional","affiliation":[{"name":"Center for Experimental and Molecular Medicine, Academic Medical Center, University of Amsterdam, Amsterdam, the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anneke","family":"Oei","sequence":"additional","affiliation":[{"name":"Center for Experimental and Molecular Medicine, Academic Medical Center, University of Amsterdam, Amsterdam, the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nadezhda M.","family":"Kolyasnikova","sequence":"additional","affiliation":[{"name":"Central Research Institute of Epidemiology, Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marina G.","family":"Toporkova","sequence":"additional","affiliation":[{"name":"Medical Association Novaya Bolnitsa, Yekaterinburg, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Denis S.","family":"Sarksyan","sequence":"additional","affiliation":[{"name":"Izhevsk State Medical Academy, Izhevsk, Udmurt Republic, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alex","family":"Wagemakers","sequence":"additional","affiliation":[{"name":"Center for Experimental and Molecular Medicine, Academic Medical Center, University of Amsterdam, Amsterdam, the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander E.","family":"Platonov","sequence":"additional","affiliation":[{"name":"Central Research Institute of Epidemiology, Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joppe W.","family":"Hovius","sequence":"additional","affiliation":[{"name":"Center for Experimental and Molecular Medicine, Academic Medical Center, University of Amsterdam, Amsterdam, the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2014.00103"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2016.90"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1710.101474"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.7326\/M15-0333"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(13)61644-X"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1209039"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMc1215469"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid2209.152034"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cll.2015.07.001"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1086\/508667"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2016.06.015"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00535-17"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2017.01.009"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/s100960050005"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2012.11.016"},{"key":"e_1_3_2_17_2","volume-title":"Document M100-S20","author":"Clinical and Laboratory Standards Institute","year":"2010","unstructured":"Clinical and Laboratory Standards Institute. 2010. Performance standards for antimicrobial susceptibility testing; 20th informational supplement. Document M100-S20. Clinical and Laboratory Standards Institute, Wayne, PA."},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1186\/1756-3305-7-418"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.3109\/00365549609049067"},{"key":"e_1_3_2_20_2","first-page":"34","article-title":"Clinical presentation of \u201cnew\u201d tick-borne borreliosis caused by Borrelia miyamotoi","volume":"84","author":"Sarksyan DS","year":"2012","unstructured":"Sarksyan DS, Platonov AE, Karan LS, Malinin IE, Khalitova LI, Shakhov VI, Dudarev MV, Malinin OV, Maleev VV. 2012. Clinical presentation of \u201cnew\u201d tick-borne borreliosis caused by Borrelia miyamotoi. Ter Arkh 84:34\u201341. (In Russian.)","journal-title":"Ter Arkh"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.00419-18","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.00419-18","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T21:15:51Z","timestamp":1645478151000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.00419-18"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,7]]},"references-count":19,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2018,7]]}},"alternative-id":["10.1128\/AAC.00419-18"],"URL":"https:\/\/doi.org\/10.1128\/aac.00419-18","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,7]]},"assertion":[{"value":"2018-03-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-04-11","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-06-26","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00419-18"}}