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In this work, we identified a novel antimicrobial peptide, LC-AMP-I1, derived from the venom of\n              <jats:italic>Lycosa coelestis<\/jats:italic>\n              , demonstrating substantial antibacterial properties and minimal hemolytic activity. LC-AMP-I1 was subjected to additional assessment for antibacterial efficacy, anti-biofilm properties, drug resistance, stability, and cytotoxicity\n              <jats:italic>in vitro<\/jats:italic>\n              . It exhibited comparable antibacterial efficacy to melittin against six common clinical multidrug-resistant bacteria, effectively inhibiting biofilm formation and disrupting established biofilms. Additionally, LC-AMP-I1 demonstrated minimal bacterial resistance, excellent stability, negligible mammalian cell toxicity, low hemolytic activity, and appropriate selectivity for both normal and tumor cells. When combined with traditional antibiotics, LC-AMP-I1 exhibited additive or synergistic therapeutic effects. In a neutropenic mouse thigh infection model, LC-AMP-I1 exhibited a therapeutic effect in inhibiting bacterial proliferation\n              <jats:italic>in vivo<\/jats:italic>\n              . The mechanistic investigation indicated that LC-AMP-I1 could influence bacterial cell membrane permeability at low concentrations and directly disrupt structure-function at high concentrations. The results of this work indicate that LC-AMP-I1 may function as a viable alternative to traditional antibiotics in addressing multidrug-resistant bacteria.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/aac.00424-24","type":"journal-article","created":{"date-parts":[[2024,12,2]],"date-time":"2024-12-02T13:42:57Z","timestamp":1733146977000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":7,"title":["LC-AMP-I1, a novel venom-derived antimicrobial peptide from the wolf spider\n            <i>Lycosa coelestis<\/i>"],"prefix":"10.1128","volume":"69","author":[{"given":"Junyao","family":"Wang","sequence":"first","affiliation":[{"name":"Institute of Biochemistry and Molecular Biology, Hengyang Medical College, University of South China","place":["Hengyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of 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