{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T16:12:01Z","timestamp":1790611921215,"version":"4.1.0"},"reference-count":44,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2010,1,1]],"date-time":"2010-01-01T00:00:00Z","timestamp":1262304000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2010,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Pseudomonas luteola<\/jats:italic>\n            (formerly classified as CDC group Ve-1 and named\n            <jats:italic>Chryseomonas luteola<\/jats:italic>\n            ) is an unusual pathogen implicated in rare but serious infections in humans. A novel \u03b2-lactamase gene,\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>LUT-1<\/jats:sub>\n            , was cloned from the whole-cell DNA of the\n            <jats:italic>P. luteola<\/jats:italic>\n            clinical isolate LAM, which had a weak narrow-spectrum \u03b2-lactam-resistant phenotype, and expressed in\n            <jats:italic>Escherichia coli<\/jats:italic>\n            . This gene encoded LUT-1, a 296-amino-acid Ambler class A \u03b2-lactamase with a pI of 6 and a theoretical molecular mass of 28.9 kDa. The catalytic efficiency of this enzyme was higher for cephalothin, cefuroxime, and cefotaxime than for penicillins. It was found to be 49% to 59% identical to other Ambler class A \u03b2-lactamases from\n            <jats:italic>Burkholderia<\/jats:italic>\n            sp. (PenA to PenL),\n            <jats:italic>Ralstonia eutropha<\/jats:italic>\n            (REUT),\n            <jats:italic>Citrobacter sedlakii<\/jats:italic>\n            (SED-1),\n            <jats:italic>Serratia fonticola<\/jats:italic>\n            (FONA and SFC-1),\n            <jats:italic>Klebsiella<\/jats:italic>\n            sp. (KPC and OXY), and CTX-M extended-spectrum \u03b2-lactamases. No gene homologous to the regulatory\n            <jats:italic>ampR<\/jats:italic>\n            genes of class A \u03b2-lactamases was found in the vicinity of the\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>LUT-1<\/jats:sub>\n            gene. The entire\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>LUT-1<\/jats:sub>\n            coding region was amplified by PCR and sequenced in five other genetically unrelated\n            <jats:italic>P. luteola<\/jats:italic>\n            strains (including the\n            <jats:italic>P. luteola<\/jats:italic>\n            type strain). A new variant of\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>LUT-1<\/jats:sub>\n            was found for each strain. These genes (named\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>LUT-2<\/jats:sub>\n            to\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>LUT-6<\/jats:sub>\n            ) had nucleotide sequences 98.1 to 99.5% identical to that of\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>LUT-1<\/jats:sub>\n            and differing from this gene by two to four nonsynonymous single nucleotide polymorphisms. The\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>LUT<\/jats:sub>\n            gene was located on a 700- to 800-kb chromosomal I-CeuI fragment, the precise size of this fragment depending on the\n            <jats:italic>P. luteola<\/jats:italic>\n            strain.\n          <\/jats:p>","DOI":"10.1128\/aac.00427-09","type":"journal-article","created":{"date-parts":[[2009,11,3]],"date-time":"2009-11-03T03:04:57Z","timestamp":1257217497000},"page":"45-51","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":17,"title":["Molecular and Biochemical Characterization of the Natural Chromosome-Encoded Class A \u03b2-Lactamase from\n            <i>Pseudomonas luteola<\/i>"],"prefix":"10.1128","volume":"54","author":[{"given":"Benoi\u0302t","family":"Doublet","sequence":"first","affiliation":[{"name":"Institut Pasteur, Laboratoire des Bacte\u0301ries Pathoge\u0300nes Ente\u0301riques, Paris, France"},{"name":"INRA, UR1282 Infectiologie Animale Sante\u0301 Publique, Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fre\u0301de\u0301ric","family":"Robin","sequence":"additional","affiliation":[{"name":"CHU de Clermont-Ferrand, Laboratoire de bacte\u0301riologie, Clermont-Ferrand, France"},{"name":"Universite\u0301 Clermont 1, UFR de Me\u0301decine, Laboratoire de Bacte\u0301riologie, JE2526 usc INRA2018, Clermont-Ferrand, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Casin","sequence":"additional","affiliation":[{"name":"Ho\u0302pital St-Louis, Service de Microbiologie, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lae\u0308titia","family":"Fabre","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Laboratoire des Bacte\u0301ries Pathoge\u0300nes Ente\u0301riques, Paris, France"},{"name":"Institut Pasteur, Unite\u0301 de Biodiversite\u0301 des Bacte\u0301ries Pathoge\u0300nes Emergentes, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Le Fleche","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Unite\u0301 de Biodiversite\u0301 des Bacte\u0301ries Pathoge\u0300nes Emergentes, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Bonnet","sequence":"additional","affiliation":[{"name":"CHU de Clermont-Ferrand, Laboratoire de bacte\u0301riologie, Clermont-Ferrand, France"},{"name":"Universite\u0301 Clermont 1, UFR de Me\u0301decine, Laboratoire de Bacte\u0301riologie, JE2526 usc INRA2018, Clermont-Ferrand, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franc\u0327ois-Xavier","family":"Weill","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Laboratoire des Bacte\u0301ries Pathoge\u0300nes Ente\u0301riques, Paris, France"},{"name":"Institut Pasteur, Unite\u0301 de Biodiversite\u0301 des Bacte\u0301ries Pathoge\u0300nes Emergentes, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.resmic.2005.02.009"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.resmic.2007.12.005"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2760269"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-47-2-249"},{"key":"e_1_3_2_6_2","first-page":"926","volume":"7","year":"1983","unstructured":"Berger, S. 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