{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,13]],"date-time":"2026-06-13T13:38:10Z","timestamp":1781357890446,"version":"3.54.1"},"reference-count":51,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2025,2,13]],"date-time":"2025-02-13T00:00:00Z","timestamp":1739404800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,2,13]],"date-time":"2025-02-13T00:00:00Z","timestamp":1739404800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2025,2,13]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              <jats:italic>Mycobacterium abscessus<\/jats:italic>\n              exhibits intrinsic resistance to most antibiotics, hence leading to infections that are difficult to treat. To address this issue, the identification of new molecular targets is essential for the development or repositioning of therapeutic agents. This study demonstrated that the\n              <jats:italic>MAB_2362<\/jats:italic>\n              -knockout strain, Mab\n              <jats:sup>\u03942362<\/jats:sup>\n              , became significantly susceptible to a range of antibiotics, not only\n              <jats:italic>in vitro<\/jats:italic>\n              but also exhibited susceptibility to rifabutin, bedaquiline, and linezolid\n              <jats:italic>in vivo<\/jats:italic>\n              . While the bacterial burden of the wild-type\n              <jats:italic>M. abscessus<\/jats:italic>\n              (Mab\n              <jats:sup>Wt<\/jats:sup>\n              ) increased by over 1 log\n              <jats:sub>10<\/jats:sub>\n              CFU\/lung in a murine infection model 16 days post-infection, that of Mab\n              <jats:sup>\u03942362<\/jats:sup>\n              strain decreased by more than 1 log\n              <jats:sub>10<\/jats:sub>\n              CFU\/lung, which suggests that the disruption leads to attenuation. Bioinformatics analysis revealed that MAB_2362 shares the highest similarity (41.35%) with SteA, a protein known to influence cell division in\n              <jats:italic>Corynebacterium glutamicum<\/jats:italic>\n              , suggesting that MAB_2362 might be involved in cell division. Mab\n              <jats:sup>\u03942362<\/jats:sup>\n              cells exhibited a median length of 2.62 \u00b5m, which was substantially longer than the 1.44 \u00b5m recorded for Mab\n              <jats:sup>Wt<\/jats:sup>\n              cells. Additionally, multiple cell division septa were observed in 42% of Mab\n              <jats:sup>\u03942362<\/jats:sup>\n              cells, whereas none were seen in Mab\n              <jats:sup>Wt<\/jats:sup>\n              cells. An ethidium bromide uptake assay further suggested a higher cell envelope permeability in Mab\n              <jats:sup>\u03942362<\/jats:sup>\n              compared to Mab\n              <jats:sup>Wt<\/jats:sup>\n              . Collectively, these findings underscore the role of\n              <jats:italic>MAB_2362<\/jats:italic>\n              in intrinsic resistance and virulence of\n              <jats:italic>M. abscessus<\/jats:italic>\n              possibly through the regulation of cell division. Thus, MAB_2362 emerges as a promising candidate for targeted interventions in the pursuit of novel antimicrobials against\n              <jats:italic>M. abscessus<\/jats:italic>\n              .\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/aac.00433-24","type":"journal-article","created":{"date-parts":[[2024,12,19]],"date-time":"2024-12-19T14:06:19Z","timestamp":1734617179000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":7,"title":["The gene\n            <i>MAB_2362<\/i>\n            is responsible for intrinsic resistance to various drugs and virulence in\n            <i>Mycobacterium abscessus<\/i>\n            by regulating cell division"],"prefix":"10.1128","volume":"69","author":[{"given":"Yanan","family":"Ju","sequence":"first","affiliation":[{"name":"School of Basic Medical Sciences, Division of Life Science and Medicine, University of Science and Technology of China","place":["Hefei, China"]},{"name":"Institute of Drug Discovery, State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences","place":["Guangzhou, China"]},{"name":"China-New Zealand Joint Laboratory on Biomedicine and Health, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijie","family":"Li","sequence":"additional","affiliation":[{"name":"School of Basic Medical Sciences, Division of Life Science and Medicine, University of Science and Technology of China","place":["Hefei, China"]},{"name":"Institute of Drug Discovery, State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences","place":["Guangzhou, China"]},{"name":"China-New Zealand Joint Laboratory on Biomedicine and Health, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingran","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Basic Medical Sciences, Division of Life Science and Medicine, University of Science and Technology of China","place":["Hefei, China"]},{"name":"Institute of Drug Discovery, State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences","place":["Guangzhou, China"]},{"name":"China-New Zealand Joint Laboratory on Biomedicine and Health, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Buhari","family":"Yusuf","sequence":"additional","affiliation":[{"name":"Institute of Drug Discovery, State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences","place":["Guangzhou, China"]},{"name":"China-New Zealand Joint Laboratory on Biomedicine and Health, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanshan","family":"Zeng","sequence":"additional","affiliation":[{"name":"Institute of Drug Discovery, State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences","place":["Guangzhou, China"]},{"name":"China-New Zealand Joint Laboratory on Biomedicine and Health, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cuiting","family":"Fang","sequence":"additional","affiliation":[{"name":"Institute of Drug Discovery, State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences","place":["Guangzhou, China"]},{"name":"China-New Zealand Joint Laboratory on Biomedicine and Health, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xirong","family":"Tian","sequence":"additional","affiliation":[{"name":"Institute of Drug Discovery, State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences","place":["Guangzhou, China"]},{"name":"China-New Zealand Joint Laboratory on Biomedicine and Health, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingli","family":"Han","sequence":"additional","affiliation":[{"name":"Institute of Drug Discovery, State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences","place":["Guangzhou, China"]},{"name":"China-New Zealand Joint Laboratory on Biomedicine and Health, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Ding","sequence":"additional","affiliation":[{"name":"Institute of Drug Discovery, State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences","place":["Guangzhou, China"]},{"name":"China-New Zealand Joint Laboratory on Biomedicine and Health, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences","place":["Guangzhou, China"]},{"name":"Institute of Physical Science and Information Technology, Anhui University","place":["Hefei, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Basic Medical Sciences, Division of Life Science and Medicine, University of Science and Technology of China","place":["Hefei, China"]},{"name":"Institute of Drug Discovery, State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences","place":["Guangzhou, China"]},{"name":"China-New Zealand Joint Laboratory on Biomedicine and Health, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanli","family":"Ma","sequence":"additional","affiliation":[{"name":"Institute of Drug Discovery, State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences","place":["Guangzhou, China"]},{"name":"China-New Zealand Joint Laboratory on Biomedicine and Health, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuai","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Drug Discovery, State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences","place":["Guangzhou, China"]},{"name":"China-New Zealand Joint Laboratory on Biomedicine and Health, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinwen","family":"Chen","sequence":"additional","affiliation":[{"name":"Guangzhou National Laboratory","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5647-6014","authenticated-orcid":true,"given":"Tianyu","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Basic Medical Sciences, Division of Life Science and Medicine, University of Science and Technology of China","place":["Hefei, China"]},{"name":"Institute of Drug Discovery, State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences","place":["Guangzhou, China"]},{"name":"China-New Zealand Joint Laboratory on Biomedicine and Health, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences","place":["Guangzhou, China"]},{"name":"Institute of Physical Science and Information Technology, Anhui University","place":["Hefei, China"]},{"name":"Guangzhou National Laboratory","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1183\/23120541.00336-2022"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms7030090"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.01739"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-020-0331-1"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1590\/0074-02760170080"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.02199-23"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciaa241"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.01977"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1812984115"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1605477113"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.046557-0"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02015-15"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiz475"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1098\/rsob.160185"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.00125"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-020-0366-3"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.GPP3-0008-2018"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.626461"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.237057"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-016-3190-4"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2007\/014076-0"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11030-023-10759-8"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1811919115"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2024.129583"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkv226"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsb.2023.01.022"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/aac.01350-22"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","unstructured":"Zeng S Ju Y Alam MS Lu Z Hameed HMA Li L Tian X Fang C Fang X Ding J Wang X Hu J Wang S Zhang T. 2024. A CRISPR-nonhomologous end-joining-based strategy for rapid and efficient gene disruption in Mycobacterium abscessus. bioRxiv. doi:10.1101\/2024.01.29.577284","DOI":"10.1101\/2024.01.29.577284"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2021.112047"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.4155\/fmc.10.220"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2014.197"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.02763-21"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciz071"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw625"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03425.x"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dku510"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.2217\/fmb-2016-0234"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.2007.24"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1080\/14787210.2020.1750951"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms9102049"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1101\/2024.03.10.584296"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.49570"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01881-19"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00330-21"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2018.02.034"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02428-14"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00550-09"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.02809-23"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1534\/g3.119.400737"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-micro-091014-104441"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1186\/s40425-018-0371-5"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aac.00433-24","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aac.00433-24","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,13]],"date-time":"2025-02-13T15:05:32Z","timestamp":1739459132000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/aac.00433-24"}},"subtitle":[],"editor":[{"given":"Sean","family":"Wasserman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,2,13]]},"references-count":51,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2025,2,13]]}},"alternative-id":["10.1128\/aac.00433-24"],"URL":"https:\/\/doi.org\/10.1128\/aac.00433-24","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,13]]},"assertion":[{"value":"2024-03-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-11-18","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-19","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00433-24"}}