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T16 accumulated inside\n                    <jats:italic>Babesia<\/jats:italic>\n                    -infected erythrocytes (cellular accumulation ratio, &gt;60) by a saturable process with an apparent\n                    <jats:italic>\n                      K\n                      <jats:sub>m<\/jats:sub>\n                    <\/jats:italic>\n                    of 0.65 \u03bcM. Subcellular fractionation of\n                    <jats:italic>Babesia<\/jats:italic>\n                    parasites revealed the accumulation of T16 into a low-density fraction, while in malaria-infected erythrocytes a significant fraction of the drug was associated with heme malaria pigment. T16 exerts an early and specific inhibition of the de novo biosynthesis of phosphatidylcholine both in\n                    <jats:italic>B. divergens-<\/jats:italic>\n                    and\n                    <jats:italic>Plasmodium falciparum<\/jats:italic>\n                    -infected erythrocytes. Choline accumulation into isolated\n                    <jats:italic>Babesia<\/jats:italic>\n                    parasites was highly sensitive to inhibition by T16. These data are consistent with the hypothesis that bisthiazolium drugs target the de novo phosphatidylcholine biosynthesis of intraerythrocytic hematozoan parasites. In malaria parasites, which generate ferriprotoporphyrin IX during hemoglobin digestion, T16 binding to heme may enhance the accumulation and activity of the drug. The selectivity of accumulation and potent activity of this class of drug into parasite-infected erythrocytes offers unique advantages over more traditional antihematozoan drugs.\n                  <\/jats:p>","DOI":"10.1128\/aac.00443-06","type":"journal-article","created":{"date-parts":[[2006,9,27]],"date-time":"2006-09-27T13:13:29Z","timestamp":1159362809000},"page":"3381-3388","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":26,"title":["Potent Antihematozoan Activity of Novel Bisthiazolium Drug T16: Evidence for Inhibition of Phosphatidylcholine Metabolism in Erythrocytes Infected with\n                    <i>Babesia<\/i>\n                    and\n                    <i>Plasmodium<\/i>\n                    spp"],"prefix":"10.1128","volume":"50","author":[{"given":"Eric","family":"Richier","sequence":"first","affiliation":[{"name":"Centre National de la Recherche Scientifique (CNRS), Unite\u0301 Mixte de Recherche (UMR) 5539"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giancarlo A.","family":"Biagini","sequence":"additional","affiliation":[{"name":"Molecular and Biochemical Parasitology Group, Liverpool School of Tropical Medicine, Liverpool, L3 5QA, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharon","family":"Wein","sequence":"additional","affiliation":[{"name":"Centre National de la Recherche Scientifique (CNRS), Unite\u0301 Mixte de Recherche (UMR) 5539"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frederic","family":"Boudou","sequence":"additional","affiliation":[{"name":"Centre National de la Recherche Scientifique (CNRS), Unite\u0301 Mixte de Recherche (UMR) 5539"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick G.","family":"Bray","sequence":"additional","affiliation":[{"name":"Molecular and Biochemical Parasitology Group, Liverpool School of Tropical Medicine, Liverpool, L3 5QA, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steve A.","family":"Ward","sequence":"additional","affiliation":[{"name":"Molecular and Biochemical Parasitology Group, Liverpool School of Tropical Medicine, Liverpool, L3 5QA, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Precigout","sequence":"additional","affiliation":[{"name":"ERT 1038 \u201cVaccination antiparasitaire\u201d, UFR Pharmacie, 15 avenue Charles Flahault, F-34093 Montpellier Cedex 05, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miche\u0300le","family":"Calas","sequence":"additional","affiliation":[{"name":"CNRS UMR 5810, Universite\u0301 Montpellier II Place E. 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