{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T03:04:22Z","timestamp":1778900662877,"version":"3.51.4"},"reference-count":44,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2016,7,1]],"date-time":"2016-07-01T00:00:00Z","timestamp":1467331200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI090155"],"award-info":[{"award-number":["R01AI090155"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R21AI117338"],"award-info":[{"award-number":["R21AI117338"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI100560"],"award-info":[{"award-number":["R01AI100560"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI063517"],"award-info":[{"award-number":["R01AI063517"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI072219"],"award-info":[{"award-number":["R01AI072219"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2016,7]]},"abstract":"ABSTRACT<\/jats:title>\n \n The emergence and spread of\n Klebsiella pneumoniae<\/jats:named-content>\n carbapenemase (KPC) among\n Enterobacteriaceae<\/jats:named-content>\n presents a major public health threat to the world. Although not as common as in\n K. pneumoniae<\/jats:named-content>\n , KPC is also found in\n Escherichia coli<\/jats:named-content>\n strains. Here, we genetically characterized 9 carbapenem-resistant\n E. coli<\/jats:named-content>\n strains isolated from six hospitals in the United States and completely sequenced their\n bla<\/jats:italic>\n KPC<\/jats:sub>\n -harboring plasmids. The nine strains were isolated from different geographical locations and belonged to 8 different\n E. coli<\/jats:named-content>\n sequence types. Seven\n bla<\/jats:italic>\n KPC<\/jats:sub>\n -harboring plasmids belonged to four different known incompatibility groups (IncN, -FIA, -FII\n K2<\/jats:sub>\n , and -FII\n K1<\/jats:sub>\n ) and ranged in size from \u223c16 kb to \u223c241 kb. In this analysis, we also identified two plasmids that have novel replicons: (i) pBK28610, which is similar to p34978-3 with an insertion of Tn\n 4401b<\/jats:italic>\n , and (ii) pBK31611, which does not have an apparent homologue in the GenBank database. Moreover, we report the emergence of a pKP048-like plasmid, pBK34397, in\n E. coli<\/jats:named-content>\n in the United States. Meanwhile, we also found examples of interspecies spread of\n bla<\/jats:italic>\n KPC<\/jats:sub>\n plasmids, as pBK34592 is identical to pBK30683, isolated from\n K. pneumoniae<\/jats:named-content>\n . In addition, we discovered examples of acquisition (pBK32602 acquired an \u223c46-kb fragment including a novel replication gene, along with Tn\n 4401b<\/jats:italic>\n and other resistance genes) and\/or loss (pKpQIL-Ec has a 14.5-kb deletion compared to pKpQIL-10 and pBK33689) of DNA, demonstrating the plasticity of these plasmids and their rapid evolution in the clinic. Overall, our study shows that the spread of\n bla<\/jats:italic>\n KPC<\/jats:sub>\n -producing\n E. coli<\/jats:named-content>\n is largely due to horizontal transfer of\n bla<\/jats:italic>\n KPC<\/jats:sub>\n -harboring plasmids and related mobile elements into diverse genetic backgrounds.\n <\/jats:p>","DOI":"10.1128\/aac.00452-16","type":"journal-article","created":{"date-parts":[[2016,4,26]],"date-time":"2016-04-26T04:08:19Z","timestamp":1461643699000},"page":"4073-4081","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":33,"title":["Molecular Diversity and Plasmid Analysis of KPC-Producing Escherichia coli"],"prefix":"10.1128","volume":"60","author":[{"given":"Kalyan D.","family":"Chavda","sequence":"first","affiliation":[{"name":"Public Health Research Institute Tuberculosis Center, New Jersey Medical School, Rutgers University, Newark, New Jersey, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liang","family":"Chen","sequence":"additional","affiliation":[{"name":"Public Health Research Institute Tuberculosis Center, New Jersey Medical School, Rutgers University, Newark, New Jersey, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael R.","family":"Jacobs","sequence":"additional","affiliation":[{"name":"Department of Pathology, University Hospitals Case Medical Center, Cleveland, Ohio, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert A.","family":"Bonomo","sequence":"additional","affiliation":[{"name":"Research Service, Louis Stokes Cleveland VA Medical Center, Cleveland, Ohio, USA"},{"name":"Departments of Medicine, Pharmacology, Molecular Biology and Microbiology, Biochemistry, Proteomics and Bioinformatics, Case Western Reserve University, Cleveland, Ohio, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barry N.","family":"Kreiswirth","sequence":"additional","affiliation":[{"name":"Public Health Research Institute Tuberculosis Center, New Jersey Medical School, Rutgers University, Newark, New Jersey, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.4.1151-1161.2001"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00041-15"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01648-12"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02749-13"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00120-14"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02587-13"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00116-14"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2014.09.003"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01019-15"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01397-13"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1086\/512370"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/1469-0691.12719"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11274-015-1897-z"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01588-10"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2006.05172.x"},{"key":"e_1_3_2_17_2","first-page":"347","article-title":"The study of optimal conditions of electroporation in Escherichia coli DH10B strain","volume":"23","author":"Zhang Y","year":"2007","unstructured":"Zhang Y, Wang ZQ, Liu B, Zhang XJ, Jiang F, Xiang JH. 2007. 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