{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T05:04:15Z","timestamp":1791003855492,"version":"4.1.0"},"reference-count":42,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2009,9,1]],"date-time":"2009-09-01T00:00:00Z","timestamp":1251763200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2009,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A retrospective survey was conducted to characterize \u03b2-lactamases in a collection of 43 ceftazidime-resistant\n            <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n            isolates recovered from patients with bloodstream infections hospitalized at a Brazilian teaching hospital between January and December 2005. Resistance rates for carbapenems, aminoglycosides, and quinolones were over 80%, with only colistin remaining active against all isolates. Pulsed-field gel electrophoresis analysis identified seven different genotypes. AmpC overproduction was found to be the sole \u03b2-lactamase-mediated mechanism responsible for ceftazidime resistance in four isolates (9.3%). Nine isolates (20.9%) produced an extended-spectrum \u03b2-lactamase (ESBL), either GES-1 (\n            <jats:italic>n<\/jats:italic>\n            = 7, 16.3%) or CTX-M-2 (\n            <jats:italic>n<\/jats:italic>\n            = 2, 4.6%). Carbapenemase activity was detected in 30 (70%) additional isolates. Among those isolates, two isolates (4.6%) produced the ESBL GES-5, possessing the ability to hydrolyze imipenem; a single isolate (2.3%) produced the metallo-\u03b2-lactamase (MBL) IMP-1; and 27 isolates produced the MBL SPM-1 (62.8%). None of the isolates coproduced both ESBL and MBL. Insertion sequence elements IS\n            <jats:italic>CR4<\/jats:italic>\n            and IS\n            <jats:italic>CR1<\/jats:italic>\n            were associated with\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>SPM-1<\/jats:sub>\n            and\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M-2<\/jats:sub>\n            genes, respectively, whereas the\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>GES-1<\/jats:sub>\n            and\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>GES-5<\/jats:sub>\n            genes were part of class 1 integron structures. This study underlines the spread of MBL- and ESBL-producing\n            <jats:italic>P. aeruginosa<\/jats:italic>\n            isolates as an important source of ceftazidime resistance in Brazil.\n          <\/jats:p>","DOI":"10.1128\/aac.00453-09","type":"journal-article","created":{"date-parts":[[2009,7,14]],"date-time":"2009-07-14T01:30:38Z","timestamp":1247535038000},"page":"3908-3913","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":91,"title":["Diversity of \u03b2-Lactamases Produced by Ceftazidime-Resistant\n            <i>Pseudomonas aeruginosa<\/i>\n            Isolates Causing Bloodstream Infections in Brazil"],"prefix":"10.1128","volume":"53","author":[{"given":"Renata C.","family":"Pica\u0303o","sequence":"first","affiliation":[{"name":"Service de Bacte\u0301riologie-Virologie, INSERM U914 Emerging Resistance to Antibiotics, Ho\u0302pital de Bice\u0302tre, Assistance Publique\/Ho\u0302pitaux de Paris, Faculte\u0301 de Me\u0301decine Paris Sud, K.-Bice\u0302tre, France"},{"name":"Laborato\u0301rio ALERTA, Universidade Federal de Sa\u0303o Paulo, Sa\u0303o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurent","family":"Poirel","sequence":"additional","affiliation":[{"name":"Service de Bacte\u0301riologie-Virologie, INSERM U914 Emerging Resistance to Antibiotics, Ho\u0302pital de Bice\u0302tre, Assistance Publique\/Ho\u0302pitaux de Paris, Faculte\u0301 de Me\u0301decine Paris Sud, K.-Bice\u0302tre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana C.","family":"Gales","sequence":"additional","affiliation":[{"name":"Laborato\u0301rio ALERTA, Universidade Federal de Sa\u0303o Paulo, Sa\u0303o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrice","family":"Nordmann","sequence":"additional","affiliation":[{"name":"Service de Bacte\u0301riologie-Virologie, INSERM U914 Emerging Resistance to Antibiotics, Ho\u0302pital de Bice\u0302tre, Assistance Publique\/Ho\u0302pitaux de Paris, Faculte\u0301 de Me\u0301decine Paris Sud, K.-Bice\u0302tre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.46704-0"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.6.2344-2345.2004"},{"key":"e_1_3_2_4_2","unstructured":"Performance standards for antimicrobial susceptibility testing. 2009"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.39.1.155"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkl517"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1179\/joc.2008.20.5.600"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.8.4129-4138.2005"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.3.638-645.2002"},{"key":"e_1_3_2_10_2","first-page":"92","year":"2008","unstructured":"El Salabi, A., M. 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