{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T13:31:52Z","timestamp":1767706312034},"reference-count":49,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2011,3,1]],"date-time":"2011-03-01T00:00:00Z","timestamp":1298937600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2011,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Porphyromonas gingivalis<\/jats:italic>\n            is a major pathogen associated with chronic periodontitis, an inflammatory disease of the supporting tissues of the teeth. The Arg-specific (RgpA\/B) and Lys-specific (Kgp) cysteine proteinases of\n            <jats:italic>P. gingivalis<\/jats:italic>\n            are major virulence factors for the bacterium. In this study \u03ba-casein(109-137) was identified in a chymosin digest of casein as an inhibiting peptide of the\n            <jats:italic>P. gingivalis<\/jats:italic>\n            proteinases. The peptide was synthesized and shown to inhibit proteolytic activity associated with\n            <jats:italic>P. gingivalis<\/jats:italic>\n            whole cells, purified RgpA-Kgp proteinase-adhesin complexes, and purified RgpB proteinase. The peptide \u03ba-casein(109-137) exhibited synergism with Zn(II) against both Arg- and Lys-specific proteinases. The active region for inhibition was identified as \u03ba-casein(117-137) using synthetic peptides. Kinetic studies revealed that \u03ba-casein(109-137) inhibits in an uncompetitive manner. A molecular model based on the uncompetitive action and its synergistic ability with Zn(II) was developed to explain the mechanism of inhibition. Preincubation of\n            <jats:italic>P. gingivalis<\/jats:italic>\n            with \u03ba-casein(109-137) significantly reduced lesion development in a murine model of infection.\n          <\/jats:p>","DOI":"10.1128\/aac.00466-10","type":"journal-article","created":{"date-parts":[[2010,12,21]],"date-time":"2010-12-21T06:06:50Z","timestamp":1292911610000},"page":"1155-1161","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":15,"title":["<i>Porphyromonas gingivalis<\/i>\n            Cysteine Proteinase Inhibition by \u03ba-Casein Peptides"],"prefix":"10.1128","volume":"55","author":[{"given":"Elena C. Y.","family":"Toh","sequence":"first","affiliation":[{"name":"Oral Health Cooperative Research Centre, Melbourne Dental School, and The Bio21 Institute of Molecular Science and Biotechnology, The University of Melbourne, 720 Swanston Street, Victoria 3010, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stuart G.","family":"Dashper","sequence":"additional","affiliation":[{"name":"Oral Health Cooperative Research Centre, Melbourne Dental School, and The Bio21 Institute of Molecular Science and Biotechnology, The University of Melbourne, 720 Swanston Street, Victoria 3010, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. Laila","family":"Huq","sequence":"additional","affiliation":[{"name":"Oral Health Cooperative Research Centre, Melbourne Dental School, and The Bio21 Institute of Molecular Science and Biotechnology, The University of Melbourne, 720 Swanston Street, Victoria 3010, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Troy J.","family":"Attard","sequence":"additional","affiliation":[{"name":"Oral Health Cooperative Research Centre, Melbourne Dental School, and The Bio21 Institute of Molecular Science and Biotechnology, The University of Melbourne, 720 Swanston Street, Victoria 3010, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neil M.","family":"O'Brien-Simpson","sequence":"additional","affiliation":[{"name":"Oral Health Cooperative Research Centre, Melbourne Dental School, and The Bio21 Institute of Molecular Science and Biotechnology, The University of Melbourne, 720 Swanston Street, Victoria 3010, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu-Yen","family":"Chen","sequence":"additional","affiliation":[{"name":"Oral Health Cooperative Research Centre, Melbourne Dental School, and The Bio21 Institute of Molecular Science and Biotechnology, The University of Melbourne, 720 Swanston Street, Victoria 3010, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keith J.","family":"Cross","sequence":"additional","affiliation":[{"name":"Oral Health Cooperative Research Centre, Melbourne Dental School, and The Bio21 Institute of Molecular Science and Biotechnology, The University of Melbourne, 720 Swanston Street, Victoria 3010, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David P.","family":"Stanton","sequence":"additional","affiliation":[{"name":"Oral Health Cooperative Research Centre, Melbourne Dental School, and The Bio21 Institute of Molecular Science and Biotechnology, The University of Melbourne, 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