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In a previous study, we described a high-throughput assay for screening for small molecules that selectively inhibit bacterial motility and identified a family of quinazoline-2,4-diamino analogs (Q24DAs) that (i) paralyzed the sodium-driven polar flagellum of<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Vibrios<\/jats:named-content>and (ii) diminished cholera toxin secreted by El Tor biotype<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">V. cholerae<\/jats:named-content>. In this study, we provide evidence that a Q24DA paralyzes the polar flagellum by interacting with the motor protein PomB. Inhibition of motility with the Q24DA enhanced the transcription of the cholera toxin genes in both biotypes. We also show that the Q24DA interacts with outer membrane protein OmpU and other porins to induce envelope stress and expression of the extracellular RNA polymerase sigma factor \u03c3<jats:sup>E<\/jats:sup>. We suggest that Q24DA-induced envelope stress could affect the correct folding, assembly, and secretion of pentameric cholera toxin in El Tor biotype<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">V. cholerae<\/jats:named-content>independently of its effect on motility.<\/jats:p>","DOI":"10.1128\/aac.00473-13","type":"journal-article","created":{"date-parts":[[2013,6,4]],"date-time":"2013-06-04T00:46:00Z","timestamp":1370306760000},"page":"3950-3959","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":11,"title":["A Quinazoline-2,4-Diamino Analog Suppresses Vibrio cholerae Flagellar Motility by Interacting with Motor Protein PomB and Induces Envelope Stress"],"prefix":"10.1128","volume":"57","author":[{"given":"Hongxia","family":"Wang","sequence":"first","affiliation":[{"name":"Southern Research Institute Drug Discovery Division, Birmingham, Alabama, USA"},{"name":"Morehouse School of Medicine Department of Microbiology, Biochemistry &amp; Immunology, Atlanta, Georgia, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Zhang","sequence":"additional","affiliation":[{"name":"Morehouse School of Medicine Department of Microbiology, Biochemistry &amp; Immunology, Atlanta, Georgia, USA"},{"name":"National Institute for Communicable Disease Control and Prevention and State Key Laboratory for Infectious Disease Prevention and Control, Chinese Center for Disease Control and Prevention, Beijing, People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anisia J.","family":"Silva","sequence":"additional","affiliation":[{"name":"Morehouse School of Medicine Department of Microbiology, Biochemistry &amp; Immunology, Atlanta, Georgia, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jorge A.","family":"Benitez","sequence":"additional","affiliation":[{"name":"Southern Research Institute Drug Discovery Division, Birmingham, Alabama, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMp1013771"},{"key":"e_1_3_2_3_2","first-page":"478","article-title":"Trend of antibiotic resistance of Vibrio cholerae strains from East Delhi","volume":"127","author":"Das S","year":"2008","unstructured":"DasS SahaR KaurIR. 2008. 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