{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T00:46:36Z","timestamp":1792457196237,"version":"4.5.0"},"reference-count":55,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100009930","name":"Basilea Pharmaceutica","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100009930","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2026,7,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Manogepix, the active moiety of the prodrug fosmanogepix, is a novel investigational agent in clinical trials that inhibits Gwt1, depleting glycosylphosphatidylinositol (GPI)-anchored mannoproteins critical for fungal cell wall integrity. It has potent broad-spectrum antifungal activity, including against fungi that are often resistant to clinically available antifungals (e.g.,\n                      <jats:italic toggle=\"yes\">Fusarium<\/jats:italic>\n                      species and\n                      <jats:italic toggle=\"yes\">Lomentospora prolificans<\/jats:italic>\n                      ). Our objective was to evaluate the\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      activity of manogepix against different\n                      <jats:italic toggle=\"yes\">Aspergillus<\/jats:italic>\n                      species,\n                      <jats:italic toggle=\"yes\">Coccidioides,<\/jats:italic>\n                      and rare molds with reduced\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      susceptibility or resistance to other antifungals. A total of 257 clinical isolates of\n                      <jats:italic toggle=\"yes\">Aspergillus, Coccidioides, Scopulariopsis\/Microascus<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">Rasamsonia argillaceae<\/jats:italic>\n                      species complex members,\n                      <jats:italic toggle=\"yes\">Purpureocillium lilacinum, Curvularia, Exophiala,<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">Exserohilum rostratum<\/jats:italic>\n                      were used. Minimum effective concentrations (MECs) (manogepix and caspofungin) and minimum inhibitory concentrations (MICs) (amphotericin B, fluconazole, isavuconazole, itraconazole, posaconazole, and fluconazole) were determined by CLSI M38 broth dilution. Manogepix demonstrated potent\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      activity against\n                      <jats:italic toggle=\"yes\">Aspergillus, Coccidioides,<\/jats:italic>\n                      and several other molds (MEC range \u2264 0.002\u20130.015 mg\/L; GM MEC range 0.003\u20130.007 mg\/L). The activity of manogepix was also maintained against strains with reduced\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      susceptibility to, or resistance to, other antifungals, including those with elevated MEC values to caspofungin, as well as isolates that were intrinsically resistant to other antifungals. Reduced or variable activity for manogepix was observed against\n                      <jats:italic toggle=\"yes\">Curvularia, Exophiala,<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">Exserohilum rostratum<\/jats:italic>\n                      . Additional studies are warranted to determine if this potent\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      activity translates into\n                      <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                      activity and enhanced clinical efficacy against infections caused by these fungal pathogens.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/aac.00476-26","type":"journal-article","created":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T13:00:41Z","timestamp":1780578041000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["Manogepix demonstrates potent\n                    <i>in vitro<\/i>\n                    activity against\n                    <i>Aspergillus, Coccidioides<\/i>\n                    ,\n                    <i>Scopulariopsis\/Microascus, Rasamsonia<\/i>\n                    , and\n                    <i>Purpureocillium<\/i>\n                    species, including strains with\n                    <i>in vitro<\/i>\n                    resistance to other antifungals"],"prefix":"10.1128","volume":"70","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2225-5122","authenticated-orcid":true,"given":"Nathan P.","family":"Wiederhold","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02f6dcw23","id-type":"ROR","asserted-by":"publisher"}],"name":"Fungus Testing Laboratory, Department of Pathology and Laboratory Medicine, University of Texas Health Science Center at San Antonio","place":["San Antonio, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hoja P.","family":"Patterson","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02f6dcw23","id-type":"ROR","asserted-by":"publisher"}],"name":"Fungus Testing Laboratory, Department of Pathology and Laboratory Medicine, University of Texas Health Science Center 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