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By employing a recently validated assay based on the release of functional phosphoglucose isomerase (PGI) from dying parasites, the activities of 26 dicationic compounds and of the (+)- and (\u2212)-<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">erythro<\/jats:named-content>-enantiomers of mefloquine were investigated. Initial screening of compounds was performed at 40 \u03bcM, and those compounds exhibiting considerable antiparasitic activities were also assessed at lower concentrations. Of the dicationic drugs, DB1127 (a diguanidino compound) with activities comparable to nitazoxanide was further studied. The activity of DB1127 was dose dependent and led to severe structural alterations, as visualized by electron microscopy. The (+)- and (\u2212)-<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">erythro<\/jats:named-content>-enantiomers of mefloquine showed similar dose-dependent effects, although higher concentrations of these compounds than of DB1127 were required for metacestode damage. In conclusion, of the drugs investigated here, the diguanidino compound DB1127 represents the most promising compound for further study in appropriate<jats:italic>in vivo<\/jats:italic>models for<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Echinococcus multilocularis<\/jats:named-content>infection.<\/jats:p>","DOI":"10.1128\/aac.00478-11","type":"journal-article","created":{"date-parts":[[2011,7,19]],"date-time":"2011-07-19T04:42:33Z","timestamp":1311050553000},"page":"4866-4872","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":36,"title":["<i>In Vitro<\/i>Efficacy of Dicationic Compounds and Mefloquine Enantiomers against Echinococcus multilocularis Metacestodes"],"prefix":"10.1128","volume":"55","author":[{"given":"Britta","family":"Stadelmann","sequence":"first","affiliation":[{"name":"Institute of Parasitology, Vetsuisse Faculty, University of Berne, L\u00e4nggass-Strasse 122, CH-3012 Berne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatiana","family":"K\u00fcster","sequence":"additional","affiliation":[{"name":"Institute of Parasitology, Vetsuisse Faculty, University of Berne, L\u00e4nggass-Strasse 122, CH-3012 Berne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabrina","family":"Scholl","sequence":"additional","affiliation":[{"name":"Institute of Parasitology, Vetsuisse Faculty, University of Berne, L\u00e4nggass-Strasse 122, CH-3012 Berne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabienne","family":"Barna","sequence":"additional","affiliation":[{"name":"Institute of Parasitology, Vetsuisse Faculty, University of Berne, L\u00e4nggass-Strasse 122, CH-3012 Berne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Kropf","sequence":"additional","affiliation":[{"name":"Institute of Parasitology, Vetsuisse Faculty, University of Berne, L\u00e4nggass-Strasse 122, CH-3012 Berne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Keiser","sequence":"additional","affiliation":[{"name":"Department of Medical Parasitology and Infection Biology, Swiss Tropical Institute, Basel Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David W.","family":"Boykin","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Georgia State University, P.O. 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