{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T00:27:12Z","timestamp":1791419232636,"version":"4.3.3"},"reference-count":58,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Unpigmented, yeast-like\n                      <jats:italic toggle=\"yes\">Prototheca<\/jats:italic>\n                      algae are emerging pathogens responsible for unusual infections in humans and animals. Their treatment is often unsuccessful, as traditional antifungals and antibacterials show unsatisfactory activity against\n                      <jats:italic toggle=\"yes\">Prototheca<\/jats:italic>\n                      . Therefore, the development of improved drugs that consider the peculiarities of algal biology is needed. Here, we describe a novel anti-\n                      <jats:italic toggle=\"yes\">Prototheca<\/jats:italic>\n                      compound, the 2-methoxy-1,4-naphthoquinone, identified among molecules known to mediate plant and algal allelopathy. The 2-methoxy-1,4-naphthoquinone was highly active\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      against a range of\n                      <jats:italic toggle=\"yes\">Prototheca<\/jats:italic>\n                      isolates from different species, both planktonically and in biofilms. Its mode of action included the induction of toxic reactive oxygen species, and it targeted energy generation in the pathogen\u2019s mitochondria. It effectively treated\n                      <jats:italic toggle=\"yes\">Prototheca bovis<\/jats:italic>\n                      skin infection in a mouse model, demonstrating translational potential. Altogether, this study not only identified a new effective anti-\n                      <jats:italic toggle=\"yes\">Prototheca<\/jats:italic>\n                      compound, but also identified 1,4-naphthoquinone backbone as a lead structure for further therapeutic development and demonstrated the feasibility of exploring known anti-plant compounds as a source of anti-\n                      <jats:italic toggle=\"yes\">Prototheca<\/jats:italic>\n                      treatments.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/aac.00497-25","type":"journal-article","created":{"date-parts":[[2025,8,27]],"date-time":"2025-08-27T14:42:58Z","timestamp":1756305778000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["Allelopathic compound 2-methoxy-1,4-naphthoquinone is broadly effective against pathogenic\n                    <i>Prototheca<\/i>\n                    species\n                    <i>in vitro<\/i>\n                    and\n                    <i>in vivo<\/i>"],"prefix":"10.1128","volume":"69","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0252-0945","authenticated-orcid":true,"given":"Amir","family":"Aliramezani","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03bqmcz70","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University","place":["Krak\u00f3w, Poland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0834-1080","authenticated-orcid":true,"given":"Grzegorz","family":"Szewczyk","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03bqmcz70","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University","place":["Krak\u00f3w, 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