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Cloning experiments identified OXA-198, a new class D \u03b2-lactamase which was weakly related (less than 45% amino acid identity) to other class D \u03b2-lactamases. Expression in<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Escherichia coli<\/jats:named-content>TOP10 and in<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">P. aeruginosa<\/jats:named-content>PAO1 led to transformants that were resistant to ticarcillin and showed reduced susceptibility to carbapenems and cefepime. The<jats:italic>bla<\/jats:italic><jats:sub>OXA-198<\/jats:sub>gene was harbored by a class 1 integron carried by a ca. 46-kb nontypeable plasmid. This study describes a novel class D \u03b2-lactamase involved in carbapenem resistance in<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">P. aeruginosa<\/jats:named-content>.<\/jats:p>","DOI":"10.1128\/aac.00522-11","type":"journal-article","created":{"date-parts":[[2011,7,26]],"date-time":"2011-07-26T03:57:58Z","timestamp":1311652678000},"page":"4828-4833","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":50,"title":["OXA-198, an Acquired Carbapenem-Hydrolyzing Class D \u03b2-Lactamase from Pseudomonas aeruginosa"],"prefix":"10.1128","volume":"55","author":[{"given":"Farid","family":"El Garch","sequence":"first","affiliation":[{"name":"Laboratoire de Bact\u00e9riologie, CHU Mont-Godinne, Universit\u00e9 Catholique de Louvain, B-5530 Yvoir, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Bogaerts","sequence":"additional","affiliation":[{"name":"Laboratoire de Bact\u00e9riologie, CHU Mont-Godinne, Universit\u00e9 Catholique de Louvain, B-5530 Yvoir, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carine","family":"Bebrone","sequence":"additional","affiliation":[{"name":"Laboratory of Biological Macromolecules, Centre for Protein Engineering, Department of Life Sciences, University of Liege, Li\u00e8ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moreno","family":"Galleni","sequence":"additional","affiliation":[{"name":"Laboratory of Biological Macromolecules, Centre for Protein Engineering, Department of Life Sciences, University of Liege, Li\u00e8ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youri","family":"Glupczynski","sequence":"additional","affiliation":[{"name":"Laboratoire de Bact\u00e9riologie, CHU Mont-Godinne, Universit\u00e9 Catholique de Louvain, B-5530 Yvoir, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00203-002-0432-4"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200405-644ST"},{"key":"e_1_3_2_4_2","volume-title":"Current protocols in molecular biology","author":"Ausubel F. 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