{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T22:11:04Z","timestamp":1790719864844,"version":"4.1.0"},"reference-count":35,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2012,10,1]],"date-time":"2012-10-01T00:00:00Z","timestamp":1349049600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2012,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The uncontrolled, often inappropriate use of antibiotics has resulted in the increasing prevalence of antibiotic-resistant pathogens, with major cost implications for both United States and European health care systems. We describe the utilization of a low-molecular-weight oligosaccharide nanomedicine (OligoG), based on the biopolymer alginate, which is able to perturb multidrug-resistant (MDR) bacteria by modulating biofilm formation and persistence and reducing resistance to antibiotic treatment, as evident using conventional and robotic MIC screening and microscopic analyses of biofilm structure. OligoG increased (up to 512-fold) the efficacy of conventional antibiotics against important MDR pathogens, including\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Pseudomonas<\/jats:named-content>\n            ,\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Acinetobacter<\/jats:named-content>\n            , and\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Burkholderia<\/jats:named-content>\n            spp., appearing to be effective with several classes of antibiotic (i.e., macrolides, \u03b2-lactams, and tetracyclines). Using confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM), increasing concentrations (2%, 6%, and 10%) of alginate oligomer were shown to have a direct effect on the quality of the biofilms produced and on the health of the cells within that biofilm. Biofilm growth was visibly weakened in the presence of 10% OligoG, as seen by decreased biomass and increased intercellular spaces, with the bacterial cells themselves becoming distorted and uneven due to apparently damaged cell membranes. This report demonstrates the feasibility of reducing the tolerance of wound biofilms to antibiotics with the use of specific alginate preparations.\n          <\/jats:p>","DOI":"10.1128\/aac.00525-12","type":"journal-article","created":{"date-parts":[[2012,7,24]],"date-time":"2012-07-24T04:37:37Z","timestamp":1343104657000},"page":"5134-5141","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":153,"title":["Overcoming Drug Resistance with Alginate Oligosaccharides Able To Potentiate the Action of Selected Antibiotics"],"prefix":"10.1128","volume":"56","author":[{"given":"Saira","family":"Khan","sequence":"first","affiliation":[{"name":"Wound Biology Group, Tissue Engineering and Reparative Dentistry, Cardiff University School of Dentistry, Cardiff, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"T\u00f8ndervik","sequence":"additional","affiliation":[{"name":"SINTEF Materials and Chemistry, Trondheim, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00e5vard","family":"Sletta","sequence":"additional","affiliation":[{"name":"SINTEF Materials and Chemistry, Trondheim, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geir","family":"Klinkenberg","sequence":"additional","affiliation":[{"name":"SINTEF Materials and Chemistry, Trondheim, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charlotte","family":"Emanuel","sequence":"additional","affiliation":[{"name":"Wound Biology Group, Tissue Engineering and Reparative Dentistry, Cardiff University School of Dentistry, Cardiff, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edvar","family":"Ons\u00f8yen","sequence":"additional","affiliation":[{"name":"AlgiPharma AS, Sandvika, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rolf","family":"Myrvold","sequence":"additional","affiliation":[{"name":"AlgiPharma AS, Sandvika, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robin A.","family":"Howe","sequence":"additional","affiliation":[{"name":"Microbiology Cardiff (Velindre NHS Trust), UHW, Heath Park, Cardiff, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy R.","family":"Walsh","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, School of Medicine, Cardiff University, Heath Park, Cardiff, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katja E.","family":"Hill","sequence":"additional","affiliation":[{"name":"Wound Biology Group, Tissue Engineering and Reparative Dentistry, Cardiff University School of Dentistry, Cardiff, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David W.","family":"Thomas","sequence":"additional","affiliation":[{"name":"Wound Biology Group, Tissue Engineering and Reparative Dentistry, Cardiff University School of Dentistry, Cardiff, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1016\/j.ijantimicag.2008.08.013","article-title":"In vitro susceptibility of established biofilms composed of a clinical wound isolate of Pseudomonas aeruginosa treated with lactoferrin and xylitol","volume":"33","author":"Ammons MCB","year":"2009","unstructured":"AmmonsMCB WardLS FisheraST WolcottRD JamesGA. 2009. 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