{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,13]],"date-time":"2023-09-13T20:15:53Z","timestamp":1694636153953},"reference-count":39,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2009,10,1]],"date-time":"2009-10-01T00:00:00Z","timestamp":1254355200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2009,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Lyme disease, the most common vector-borne zoonosis in North America, is caused by the spirochetal pathogen\n            <jats:italic>Borrelia burgdorferi<\/jats:italic>\n            . The telomere resolvase encoded by this organism (ResT) promotes the formation of covalently closed hairpin ends on the linear DNA molecules of\n            <jats:italic>B. burgdorferi<\/jats:italic>\n            through a two-step transesterification. ResT is essential for survival and is therefore an attractive target for the development of highly specific antiborrelial drugs. To identify ResT inhibitors, a novel fluorescence-based high-throughput assay was developed and used to screen a library of 27,520 small-molecule drug-like compounds. Six confirmed inhibitors of ResT, with 50% inhibitory concentrations between 2 and 10 \u03bcM, were identified. The inhibitors were characterized further and were grouped into three distinct classes based on their inhibitory features. The high-throughput screening assay developed in this paper, along with the six inhibitory compounds identified, provides a starting point for the future development of novel antiborrelial drugs as well as small-molecule inhibitors that will be helpful for the further dissection of the reaction mechanism.\n          <\/jats:p>","DOI":"10.1128\/aac.00529-09","type":"journal-article","created":{"date-parts":[[2009,7,14]],"date-time":"2009-07-14T01:30:38Z","timestamp":1247535038000},"page":"4441-4449","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":7,"title":["High-Throughput Screening Identifies Three Inhibitor Classes of the Telomere Resolvase from the Lyme Disease Spirochete"],"prefix":"10.1128","volume":"53","author":[{"given":"Georgia","family":"Lefas","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology and Department of Microbiology and Infectious Diseases, University of Calgary, 3330 Hospital Dr. N.W., Calgary, Alberta T2N 4N1, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"George","family":"Chaconas","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology and Department of Microbiology and Infectious Diseases, University of Calgary, 3330 Hospital Dr. N.W., Calgary, Alberta T2N 4N1, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0405762101"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2006.05423.x"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3603026"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.04688.x"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.9.2445-2452.2000"},{"key":"e_1_3_2_7_2","first-page":"119","volume":"95","year":"2001","unstructured":"Burgin, A. 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