{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,9]],"date-time":"2025-04-09T04:47:36Z","timestamp":1744174056664},"reference-count":7,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2009,2,1]],"date-time":"2009-02-01T00:00:00Z","timestamp":1233446400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2009,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Recently the CLSI recommended a disk diffusion method and breakpoints for meningococci which include breakpoints derived for nalidixic acid which serve as surrogate markers for gyrase A mutations associated with diminished fluoroquinolone susceptibility. This study presents the application of this methodology to a panel of 57 meningococcal strains isolated in Spain that include all levels of susceptibility to ciprofloxacin. In conclusion, the most useful method to predict isolates with\n            <jats:italic>gyrA<\/jats:italic>\n            mutations that decrease the activity of fluoroquinolones is the use of 30-\u03bcg nalidixic acid disks.\n          <\/jats:p>","DOI":"10.1128\/aac.00531-08","type":"journal-article","created":{"date-parts":[[2008,11,18]],"date-time":"2008-11-18T02:26:03Z","timestamp":1226975163000},"page":"796-797","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["Nalidixic Acid Disk for Laboratory Detection of Ciprofloxacin Resistance in\n            <i>Neisseria meningitidis<\/i>"],"prefix":"10.1128","volume":"53","author":[{"given":"Roci\u0301o","family":"Enri\u0301quez","sequence":"first","affiliation":[{"name":"Reference Laboratory for Meningococci-Servicio de Bacteriologi\u0301a, National Centre for Microbiology, National Institute of Health Carlos III, Majadahonda (Madrid), Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raquel","family":"Abad","sequence":"additional","affiliation":[{"name":"Reference Laboratory for Meningococci-Servicio de Bacteriologi\u0301a, National Centre for Microbiology, National Institute of Health Carlos III, Majadahonda (Madrid), Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Celia","family":"Salcedo","sequence":"additional","affiliation":[{"name":"Reference Laboratory for Meningococci-Servicio de Bacteriologi\u0301a, National Centre for Microbiology, National Institute of Health Carlos III, Majadahonda (Madrid), Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julio A.","family":"Va\u0301zquez","sequence":"additional","affiliation":[{"name":"Reference Laboratory for Meningococci-Servicio de Bacteriologi\u0301a, National Centre for Microbiology, National Institute of Health Carlos III, Majadahonda (Madrid), Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"173","volume":"57","year":"2008","unstructured":"Centers for Disease Control and Prevention. 2008. Emergence of fluoroquinolone-resistant Neisseria meningitidis\u2014Minnesota and North Dakota, 2007-2008. MMWR Morb. Mortal. Wkly. Rep.57:173-175.","journal-title":"MMWR Morb. Mortal. Wkly. Rep."},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2006.11.028"},{"key":"e_1_3_2_4_2","unstructured":"Supplement M100-S16. 2007"},{"key":"e_1_3_2_5_2","first-page":"286","volume":"61","year":"2008","unstructured":"Enr\u00edquez, R., R. Abad, C. Salcedo, S. P\u00e9rez, and J. A. V\u00e1zquez. 2008. Fluoroquinolone resistance in Neisseria meningitidis in Spain. J. Antimicrob. Chemother.61:286-290.","journal-title":"J. Antimicrob. Chemother."},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.44.5.1744-1754.2006"},{"key":"e_1_3_2_7_2","doi-asserted-by":"crossref","first-page":"1614","DOI":"10.3201\/eid1310.060820","volume":"13","year":"2007","unstructured":"Singhal, S., K. P. Purnapatre, V. Kalia, S. Dube, D. Nair, M. Deb, P. Aggarwal, S. Gupta, D. J. Upadhyay, A. Rattan, and V. S. Raj. 2007. Ciprofloxacin-resistant Neisseria meningitidis, Delhi, India. Emerg. Infect. Dis.13:1614-1616.","journal-title":"Emerg. Infect. Dis."},{"key":"e_1_3_2_8_2","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1111\/j.1574-6976.2006.00050.x","volume":"31","year":"2007","unstructured":"V\u00e1zquez, J. A. 2007. Resistance testing of meningococci: the recommendations of the European Monitoring Group on Meningococci. FEMS Microbiol. Rev.31:97-100.","journal-title":"FEMS Microbiol. Rev."}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.00531-08","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.00531-08","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T19:51:17Z","timestamp":1645473077000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.00531-08"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,2]]},"references-count":7,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2009,2]]}},"alternative-id":["10.1128\/AAC.00531-08"],"URL":"https:\/\/doi.org\/10.1128\/aac.00531-08","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,2]]},"assertion":[{"value":"2008-04-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2008-11-08","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-02-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}