{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T13:47:00Z","timestamp":1762004820798,"version":"3.40.5"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2016,12,1]],"date-time":"2016-12-01T00:00:00Z","timestamp":1480550400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"National Key Program of Mega Infectious Diseases","award":["2013ZX10003002-004"],"award-info":[{"award-number":["2013ZX10003002-004"]}]},{"name":"Scientific Research Fund Project of Department of Health of Hebei Province","award":["20130650"],"award-info":[{"award-number":["20130650"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Ethambutol (EMB) is an essential first-line drug for tuberculosis (TB) treatment. Nucleotide substitutions at\n            <jats:italic>embB<\/jats:italic>\n            codon 306 (\n            <jats:italic>embB<\/jats:italic>\n            306) have been proposed to be a potential marker for EMB resistance and a predictor of broad drug resistance in clinical\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Mycobacterium tuberculosis<\/jats:named-content>\n            isolates. However, discordant findings about the association between\n            <jats:italic>embB<\/jats:italic>\n            306 mutations and EMB resistance were reported. Hebei Province is located in the Beijing-Tianjin-Hebei integration region in China; however, little information about the genetic diversity of the\n            <jats:italic>embB<\/jats:italic>\n            locus in this area is available. In this study, we sequenced the region surrounding\n            <jats:italic>embB<\/jats:italic>\n            306 (codons 207 to 445) in 62 ethambutol-resistant (EMB\n            <jats:sup>r<\/jats:sup>\n            ) isolates, 214 ethambutol-susceptible isolates resistant to other first-line drugs (EMB\n            <jats:sup>s<\/jats:sup>\n            isolates), and 100 pan-sensitive isolates. Our data indicated that none of the pan-sensitive isolates showed mutations at\n            <jats:italic>embB<\/jats:italic>\n            306 and 63 drug-resistant isolates harbored\n            <jats:italic>embB<\/jats:italic>\n            306 substitutions, with these substitutions being found in 56.5% (35\/62) of EMB\n            <jats:sup>r<\/jats:sup>\n            isolates and 13.1% (28\/214) of EMB\n            <jats:sup>s<\/jats:sup>\n            isolates. A significant association between the\n            <jats:italic>embB<\/jats:italic>\n            306 mutation and resistance to isoniazid, rifampin, EMB, and multiple drugs was observed, and the rate of mutation of\n            <jats:italic>embB<\/jats:italic>\n            306 increased with increasing numbers of first-line drugs to which the isolates were resistant. The\n            <jats:italic>embB<\/jats:italic>\n            306 mutation is not the sole causative factor for EMB resistance, and the poor sensitivity limits its utility as a marker for drug-resistant TB. However, it may be a potential marker for broad drug resistance, especially for multidrug resistance. The mycobacterial interspersed repetitive unit\u2013variable-number tandem-repeat profiles may serve as markers for predicting the\n            <jats:italic>embB<\/jats:italic>\n            306 substitutions that may occur in drug-resistant\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">M. tuberculosis<\/jats:named-content>\n            isolates under antimicrobial selection pressure.\n          <\/jats:p>","DOI":"10.1128\/aac.00532-16","type":"journal-article","created":{"date-parts":[[2016,9,27]],"date-time":"2016-09-27T03:44:12Z","timestamp":1474947852000},"page":"7295-7302","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":7,"title":["Association between\n            <i>embB<\/i>\n            Codon 306 Mutations, Phenotypic Resistance Profiles, and Genotypic Characterization in Clinical Mycobacterium tuberculosis Isolates from Hebei, China"],"prefix":"10.1128","volume":"60","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4982-9373","authenticated-orcid":false,"given":"Yanan","family":"Li","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, the Fifth Hospital of Shijiazhuang, Hebei Medical University, Shijiazhuang, Hebei, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuling","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Tuberculosis, the Fifth Hospital of Shijiazhuang, Hebei Medical University, Shijiazhuang, Hebei, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, the Fifth Hospital of Shijiazhuang, Hebei Medical University, Shijiazhuang, Hebei, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huixia","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, the Fifth Hospital of Shijiazhuang, Hebei Medical University, Shijiazhuang, Hebei, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haibin","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Tuberculosis, the Fifth Hospital of Shijiazhuang, Hebei Medical University, Shijiazhuang, Hebei, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinfeng","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Tuberculosis, the Fifth Hospital of Shijiazhuang, Hebei Medical University, Shijiazhuang, Hebei, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shumin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Tuberculosis, the Fifth Hospital of Shijiazhuang, Hebei Medical University, Shijiazhuang, Hebei, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuzhen","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, the Fifth Hospital of Shijiazhuang, Hebei Medical University, Shijiazhuang, Hebei, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianhua","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, the Fifth Hospital of Shijiazhuang, Hebei Medical University, Shijiazhuang, Hebei, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zungui","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Tuberculosis, the Fifth Hospital of Shijiazhuang, Hebei Medical University, Shijiazhuang, Hebei, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erhei","family":"Dai","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, the Fifth Hospital of Shijiazhuang, Hebei Medical University, Shijiazhuang, Hebei, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","unstructured":"World Health Organization. 2015. Global tuberculosis report. World Health Organization Geneva Switzerland. http:\/\/www.who.int\/tb\/publications\/global_report\/gtbr15_main_text.pdf."},{"key":"e_1_3_3_3_2","first-page":"1","article-title":"Treatment of tuberculosis","volume":"52","author":"American Thoracic Society, CDC, Infectious Diseases Society of America","year":"2003","unstructured":"American Thoracic Society, CDC, Infectious Diseases Society of America. 2003. Treatment of tuberculosis. MMWR Recommend Rep 52 (RR-11):1\u201377.","journal-title":"MMWR Recommend Rep"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm0597-567"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03681.x"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.50.5.1900-1902.2006"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.04933-14"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.9.3794-3802.2005"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00117-15"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01550-10"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkr284"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01516-06"},{"key":"e_1_3_3_13_2","volume-title":"The 2000 National Tuberculosis Epidemiological Survey data compilation, p 15\u201316","author":"Ministry of Health of the People's Republic of China","year":"2003","unstructured":"Ministry of Health of the People's Republic of China. 2003. The 2000 National Tuberculosis Epidemiological Survey data compilation, p 15\u201316. People's Medical Publishing House, Beijing, China."},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.4.907-914.1997"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01392-06"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.10.3810-3813.2002"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.resmic.2004.06.007"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkt448"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00150-15"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.3349\/ymj.2002.43.1.59"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.39.11.2484"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.11.4447-4449.2004"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2008.09.021"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng.2743"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.41.10.2270"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.2.326-336.2000"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01486-07"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00007-10"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.6.2218-2225.2005"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01357-08"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-016-1441-2"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.00532-16","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.00532-16","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T20:28:30Z","timestamp":1645475310000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.00532-16"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,12]]},"references-count":30,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2016,12]]}},"alternative-id":["10.1128\/AAC.00532-16"],"URL":"https:\/\/doi.org\/10.1128\/aac.00532-16","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"type":"print","value":"0066-4804"},{"type":"electronic","value":"1098-6596"}],"subject":[],"published":{"date-parts":[[2016,12]]},"assertion":[{"value":"2016-03-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-09-20","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-11-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}