{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T17:43:12Z","timestamp":1781199792146,"version":"3.54.1"},"reference-count":40,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2007,11,1]],"date-time":"2007-11-01T00:00:00Z","timestamp":1193875200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2007,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A chromosomal \u03b2-lactamase gene from\n            <jats:italic>Bacillus clausii<\/jats:italic>\n            NR, which is used as a probiotic, was cloned and expressed in\n            <jats:italic>Escherichia coli<\/jats:italic>\n            . It encodes a clavulanic acid-susceptible Ambler class A \u03b2-lactamase, BCL-1, with a pI of 5.5 and a molecular mass of ca. 32 kDa. It shares 91% and 62% amino acid identity with the chromosomally encoded PenP penicillinases from\n            <jats:italic>B. clausii<\/jats:italic>\n            KSM-K16 and\n            <jats:italic>Bacillus licheniformis<\/jats:italic>\n            , respectively. The hydrolytic profile of this \u03b2-lactamase includes penicillins, narrow-spectrum cephalosporins, and cefpirome. This chromosome-encoded enzyme was inducible in\n            <jats:italic>B. clausii<\/jats:italic>\n            , and its gene is likely related to upstream-located regulatory genes that share significant identity with those reported to be upstream of the penicillinase gene of\n            <jats:italic>B. licheniformis<\/jats:italic>\n            . The\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>BCL-1<\/jats:sub>\n            gene was located next to the known chromosomal\n            <jats:italic>aadD2<\/jats:italic>\n            gene and the\n            <jats:italic>erm34<\/jats:italic>\n            gene, which encode resistance to aminoglycosides and macrolides, respectively. Similar genes were found in a collection of\n            <jats:italic>B. clausii<\/jats:italic>\n            reference strains.\n          <\/jats:p>","DOI":"10.1128\/aac.00537-07","type":"journal-article","created":{"date-parts":[[2007,9,11]],"date-time":"2007-09-11T00:55:20Z","timestamp":1189472120000},"page":"4009-4014","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":26,"title":["Molecular and Biochemical Characterization of the Chromosome-Encoded Class A \u03b2-Lactamase BCL-1 from\n            <i>Bacillus clausii<\/i>"],"prefix":"10.1128","volume":"51","author":[{"given":"Delphine","family":"Girlich","sequence":"first","affiliation":[{"name":"Service de Bacte\u0301riologie-Virologie, Ho\u0302pital de Bice\u0302tre, Assistance Publique\/Ho\u0302pitaux de Paris, Faculte\u0301 de Me\u0301decine Paris-Sud, 94275 Le Kremlin-Bice\u0302tre"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roland","family":"Leclercq","sequence":"additional","affiliation":[{"name":"Service de Microbiologie, Hopital Co\u0302te de Nacre, Universite\u0301 de Caen, 14033 Caen Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thierry","family":"Naas","sequence":"additional","affiliation":[{"name":"Service de Bacte\u0301riologie-Virologie, Ho\u0302pital de Bice\u0302tre, Assistance Publique\/Ho\u0302pitaux de Paris, Faculte\u0301 de Me\u0301decine Paris-Sud, 94275 Le Kremlin-Bice\u0302tre"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrice","family":"Nordmann","sequence":"additional","affiliation":[{"name":"Service de Bacte\u0301riologie-Virologie, Ho\u0302pital de Bice\u0302tre, Assistance Publique\/Ho\u0302pitaux de Paris, Faculte\u0301 de Me\u0301decine Paris-Sud, 94275 Le Kremlin-Bice\u0302tre"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2760269"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.8.1924-1930.1997"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.2.968-978.2005"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.4.1343-1346.2003"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.1.280-284.2004"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.11.2720"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.39.6.1211"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2006.03042.x"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.3.823-830.2003"},{"key":"e_1_3_2_11_2","first-page":"45","volume":"3","year":"1984","unstructured":"Ciffo, F. 1984. 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