{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:27:24Z","timestamp":1787016444104,"version":"build-2736575974"},"reference-count":18,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2018,8,1]],"date-time":"2018-08-01T00:00:00Z","timestamp":1533081600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Professor Tsuei-Chu Mong Merit Scholarship","award":["40419001"],"award-info":[{"award-number":["40419001"]}]},{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","award":["MOST 104-2320-B-010-023-MY3"],"award-info":[{"award-number":["MOST 104-2320-B-010-023-MY3"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The linkage of the protease-chaperon system, SmeYZ pump, and aminoglycoside resistance was assessed in\n                    <jats:named-content content-type=\"genus-species\">Stenotrophomonas maltophilia<\/jats:named-content>\n                    . The\n                    <jats:italic>clpA<\/jats:italic>\n                    ,\n                    <jats:italic>clpS<\/jats:italic>\n                    ,\n                    <jats:italic>clpP<\/jats:italic>\n                    , and\n                    <jats:italic>htpX<\/jats:italic>\n                    genes were upregulated in response to kanamycin exposure. Of these,\n                    <jats:italic>clpA<\/jats:italic>\n                    and\n                    <jats:italic>htpX<\/jats:italic>\n                    were the primary determinants responsible for intrinsic aminoglycoside (AG) resistance. Inactivation of\n                    <jats:italic>clpA<\/jats:italic>\n                    and\n                    <jats:italic>htpX<\/jats:italic>\n                    compromised protease-mediated intrinsic aminoglycoside resistance and weakened SmeYZ pump-mediated aminoglycoside resistance, signifying HtpX and ClpA as potential AG adjuvant targets for treatment of\n                    <jats:named-content content-type=\"genus-species\">S. maltophilia<\/jats:named-content>\n                    infections.\n                  <\/jats:p>","DOI":"10.1128\/aac.00554-18","type":"journal-article","created":{"date-parts":[[2018,5,11]],"date-time":"2018-05-11T05:33:42Z","timestamp":1526016822000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":22,"title":["ClpA and HtpX Proteases Are Involved in Intrinsic Aminoglycoside Resistance of Stenotrophomonas maltophilia and Are Potential Aminoglycoside Adjuvant Targets"],"prefix":"10.1128","volume":"62","author":[{"given":"Hsin-Hui","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Tsung","family":"Lin","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, Taipei Veterans General Hospital, Taipei, Taiwan"},{"name":"School of Medicine, National Yang-Ming University, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng-Ying","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li-Hua","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Taipei Veterans General Hospital, Taipei, Taiwan"},{"name":"School of Medical Laboratory Science and Biotechnology, College of Medical Science and Technology, Taipei Medical University, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hsiao-Chen","family":"Ning","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Chang Gung Memorial Hospital Linkou Branch, Taoyuan, Taiwan"},{"name":"Department of Medical Biotechnology and Laboratory Science, Chang Gung University, Taoyuan, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsuey-Ching","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a004366"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1515\/hsz-2016-0302"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M506180200"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.01951.x"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb1271"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1039\/C5MD00344J"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.05133-11"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.resmic.2017.06.002"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-43-3-606"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.00658"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01513-08"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.020339-0"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2008.09.038"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.5.1915-1926.2005"},{"key":"e_1_3_2_17_2","volume-title":"CLSI supplement M100","author":"Clinical and Laboratory Standards Institute","year":"2017","unstructured":"Clinical and Laboratory Standards Institute. 2017. performance standards for antimicrobial susceptibility testing, 27th ed. 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