{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T15:18:26Z","timestamp":1788189506543,"version":"build-2803163510"},"reference-count":21,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2015,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We examined the genetic context of 74 acquired\n            <jats:italic>ampC<\/jats:italic>\n            genes and 17 carbapenemase genes from 85 of 640\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Enterobacteriaceae<\/jats:named-content>\n            isolates collected in 2009. Using S1 pulsed-field gel electrophoresis and Southern hybridization, 37 of 74\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>AmpC<\/jats:sub>\n            genes were located on large plasmids of different sizes belonging to six incompatibility groups. We used sequencing and PCR mapping to investigate the regions flanking the acquired\n            <jats:italic>ampC<\/jats:italic>\n            genes. The\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CMY-2<\/jats:sub>\n            -like genes were associated with IS\n            <jats:italic>Ecp1<\/jats:italic>\n            ; the surrounding\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>DHA<\/jats:sub>\n            genes were similar to\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Klebsiella pneumoniae<\/jats:named-content>\n            plasmid pTN60013 associated with IS\n            <jats:italic>26<\/jats:italic>\n            and the\n            <jats:italic>psp<\/jats:italic>\n            and\n            <jats:italic>sap<\/jats:italic>\n            operons; and the\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>ACC-1<\/jats:sub>\n            genes were associated with IS\n            <jats:italic>26<\/jats:italic>\n            elements inserted into IS\n            <jats:italic>Ecp1<\/jats:italic>\n            . All of the carbapenemase genes (\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>VIM-1<\/jats:sub>\n            ,\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>IMP-22<\/jats:sub>\n            , and\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>IMP-28<\/jats:sub>\n            ) were located in class 1 integrons. Therefore, although plasmids are the main cause of the rapid dissemination of\n            <jats:italic>ampC<\/jats:italic>\n            genes among\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Enterobacteriaceae<\/jats:named-content>\n            , we need to be aware that other mobile genetic elements, such as insertion sequences, transposons, or integrons, can be involved in the mobilization of these genes of chromosomal origin. Additionally, three new integrons (In846 to In848) are described in this study.\n          <\/jats:p>","DOI":"10.1128\/aac.00562-15","type":"journal-article","created":{"date-parts":[[2015,6,16]],"date-time":"2015-06-16T03:01:14Z","timestamp":1434423674000},"page":"5260-5266","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":21,"title":["Mobile Genetic Elements Related to the Diffusion of Plasmid-Mediated AmpC \u03b2-Lactamases or Carbapenemases from Enterobacteriaceae: Findings from a Multicenter Study in Spain"],"prefix":"10.1128","volume":"59","author":[{"given":"L.","family":"Zamorano","sequence":"first","affiliation":[{"name":"Servicio de Microbiolog\u00eda and Unidad de Investigaci\u00f3n, Hospital Universitario Son Espases, Instituto de Investigaci\u00f3n Sanitaria de Palma (IdISPa), Palma de Mallorca, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Mir\u00f3","sequence":"additional","affiliation":[{"name":"Servei de Microbiologia, Hospital de la Santa Creu i Sant Pau, Institut d'Investigaci\u00f3 Biom\u00e8dica Sant Pau, Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Juan","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda and Unidad de Investigaci\u00f3n, Hospital Universitario Son Espases, Instituto de Investigaci\u00f3n Sanitaria de Palma (IdISPa), Palma de Mallorca, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"G\u00f3mez","sequence":"additional","affiliation":[{"name":"Servei de Microbiologia, Hospital de la Santa Creu i Sant Pau, Institut d'Investigaci\u00f3 Biom\u00e8dica Sant Pau, Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Bou","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda, Complejo Hospitalario Universitario a Coru\u00f1a, A Coru\u00f1a, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. J.","family":"Gonz\u00e1lez-L\u00f3pez","sequence":"additional","affiliation":[{"name":"Servei de Microbiologia, Hospital Vall d'Hebr\u00f3n, Barcelona, Spain"},{"name":"Departament de Gen\u00e8tica i Microbiologia, Universitat Aut\u00f2noma de Barcelona, Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Mart\u00ednez-Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda, Hospital Universitario Marqu\u00e9s de Valdecilla-IDIVAL, and Departamento de Biolog\u00eda Molecular, Universidad de Cantabria, Cantabria, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Aracil","sequence":"additional","affiliation":[{"name":"Laboratorio de Antibi\u00f3ticos, Servicio de Bacteriolog\u00eda, Centro Nacional de Microbiolog\u00eda, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. C.","family":"Conejo","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda, Facultad de Medicina, Universidad de Sevilla, Sevilla, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Oliver","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda and Unidad de Investigaci\u00f3n, Hospital Universitario Son Espases, Instituto de Investigaci\u00f3n Sanitaria de Palma (IdISPa), Palma de Mallorca, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4302-2838","authenticated-orcid":false,"given":"F.","family":"Navarro","sequence":"additional","affiliation":[{"name":"Servei de Microbiologia, Hospital de la Santa Creu i Sant Pau, Institut d'Investigaci\u00f3 Biom\u00e8dica Sant Pau, Barcelona, Spain"},{"name":"Departament de Gen\u00e8tica i Microbiologia, Universitat Aut\u00f2noma de Barcelona, Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01896-13"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01686-12"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-012-1737-0"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2013.02.001"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2010.01980.x"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2005.03.018"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkr412"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00753-08"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.46151-0"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.50.4.1590-1593.2006"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.50.2.607-617.2006"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00364-07"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00619-06"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00810-07"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00818-13"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00776-12"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00137-10"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2009.02861.x"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2012.1130"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00149-06"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2010.03.019"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.00562-15","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.00562-15","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T22:58:58Z","timestamp":1645484338000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.00562-15"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,9]]},"references-count":21,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2015,9]]}},"alternative-id":["10.1128\/AAC.00562-15"],"URL":"https:\/\/doi.org\/10.1128\/aac.00562-15","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,9]]},"assertion":[{"value":"2015-03-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-06-08","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-08-14","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}