{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T01:42:55Z","timestamp":1768700575874,"version":"3.49.0"},"reference-count":36,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2011,10,1]],"date-time":"2011-10-01T00:00:00Z","timestamp":1317427200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2011,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>The purpose of the present study was to evaluate the toxicity of voriconazole on cultured human corneal endothelial cells (HCECs). HCECs were cultured and exposed to various concentrations of voriconazole (5.0 to 1,000 \u03bcg\/ml). Cell viability was measured using a Cell Counting Kit-8 (CCK-8) and live\/dead viability\/cytotoxicity assays. Cell damage was assessed using phase-contrast microscopy after 24 h of exposure to voriconazole. To analyze the effect of voriconazole on the intercellular barrier, immunolocalization of zonula occludens 1 (ZO1) was performed. A flow cytometric assay was performed to evaluate the apoptotic and necrotic effects of voriconazole on HCECs. Cytotoxicity tests demonstrated the dose-dependent toxic effect of voriconazole on HCECs. Voriconazole concentrations of \u2265100 \u03bcg\/ml led to a significant reduction in cell viability. The morphological characteristics of HCECs also changed in a dose-dependent manner. Increasing concentrations of voriconazole resulted in fading staining for ZO1. Higher concentrations of voriconazole resulted in an increased number of propidium iodide (PI)-positive cells, indicating activation of the proapoptotic pathway. In conclusion, voriconazole may have a dose-dependent toxic effect on cultured HCECs. The results of this study suggest that although voriconazole concentrations of up to 50 \u03bcg\/ml do not decrease cell viability, intracameral voriconazole concentrations of \u2265100 \u03bcg\/ml may increase the risk of corneal endothelial damage.<\/jats:p>","DOI":"10.1128\/aac.00569-11","type":"journal-article","created":{"date-parts":[[2011,7,19]],"date-time":"2011-07-19T04:42:33Z","timestamp":1311050553000},"page":"4519-4523","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":44,"title":["Cytotoxicity of Voriconazole on Cultured Human Corneal Endothelial Cells"],"prefix":"10.1128","volume":"55","author":[{"given":"Sang Beom","family":"Han","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, Seoul National University Bundang Hospital, Seongnam, South Korea"},{"name":"Department of Ophthalmology, Seoul National University College of Medicine, Seoul, South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Young Joo","family":"Shin","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Hallym University College of Medicine, Kangnam Sacred Heart Hospital, Seoul, South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joon Young","family":"Hyon","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Seoul National University Bundang Hospital, Seongnam, South Korea"},{"name":"Department of Ophthalmology, Seoul National University College of Medicine, Seoul, South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Won Ryang","family":"Wee","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Seoul National University College of Medicine, Seoul, South Korea"},{"name":"Seoul Artificial Eye Center, Seoul National University Hospital Clinical Research Institute, Seoul, South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1345\/aph.1M008"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0420.2000.078002130.x"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00228-010-0914-2"},{"key":"e_1_3_2_5_2","first-page":"911","article-title":"Reactive oxygen species-induced apoptosis and necrosis in bovine corneal endothelial cells","volume":"40","author":"Cho K. S.","year":"1999","unstructured":"ChoK. S. LeeE. H. ChoiJ. S. JooC. K.. 1999. Reactive oxygen species-induced apoptosis and necrosis in bovine corneal endothelial cells. Invest. Ophthalmol. Vis. Sci. 40:911\u2013919.","journal-title":"Invest. Ophthalmol. Vis. Sci"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/43.1.149"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkl108"},{"key":"e_1_3_2_8_2","first-page":"56","article-title":"Isolation and long-term cultivation of human corneal endothelial cells","volume":"29","author":"Engelmann K.","year":"1988","unstructured":"EngelmannK. BohnkeM. FriedlP.. 1988. Isolation and long-term cultivation of human corneal endothelial cells. Invest. Ophthalmol. Vis. Sci. 29:56\u20131662.","journal-title":"Invest. Ophthalmol. Vis. Sci"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.122.11.1687"},{"key":"e_1_3_2_10_2","first-page":"242","article-title":"Voriconazole\u2014better chances for patients with invasive mycoses","volume":"7","author":"Ghannoum M. A.","year":"2002","unstructured":"GhannoumM. A. KuhnD. M.. 2002. Voriconazole\u2014better chances for patients with invasive mycoses. Eur. J. Med. Res. 7:242\u2013256.","journal-title":"Eur. J. Med. Res"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.12.4.501"},{"key":"e_1_3_2_12_2","first-page":"47","article-title":"Determination of vitreous, aqueous, and plasma concentration of orally administered voriconazole in humans","volume":"122","author":"Hariprasad S. M.","year":"2004","unstructured":"HariprasadS. M. . 2004. Determination of vitreous, aqueous, and plasma concentration of orally administered voriconazole in humans. Arch. Ophthalmol. 122:47.","journal-title":"Arch. Ophthalmol"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.19.1518"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2008.05.023"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1086\/367933"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.ico.0000136666.63870.18"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2009.01.008"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1097\/IAE.0b013e31818d4b9b"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2006.01.059"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1177\/00912700222011445"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkm330"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1089\/jop.2007.0105"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.1.250-253.2005"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.3.1087-1090.2003"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1086\/374557"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.3.1126-1129.2003"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.5.1875-1880.2003"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.84.8.928d"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.iae.0000250011.68532.a2"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01125-08"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.06-1362"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00762-06"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1001\/archophthalmol.2007.8"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(95)00072-I"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00587-10"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1097\/ICO.0b013e318030767e"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.07-1079"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.00569-11","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.00569-11","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T21:55:12Z","timestamp":1645480512000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.00569-11"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,10]]},"references-count":36,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2011,10]]}},"alternative-id":["10.1128\/AAC.00569-11"],"URL":"https:\/\/doi.org\/10.1128\/aac.00569-11","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,10]]},"assertion":[{"value":"2011-04-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-07-08","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-09-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}