{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T11:33:39Z","timestamp":1780572819265,"version":"3.54.1"},"reference-count":51,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2023,11,15]],"date-time":"2023-11-15T00:00:00Z","timestamp":1700006400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/doi.org\/10.1128\/ASMCopyrightv2"},{"start":{"date-parts":[[2023,11,15]],"date-time":"2023-11-15T00:00:00Z","timestamp":1700006400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"UofI | University of Illinois at Urbana-Champaign","award":["UIUC-HUJI Joint Seed grant"],"award-info":[{"award-number":["UIUC-HUJI Joint Seed grant"]}]},{"DOI":"10.13039\/501100003483","name":"Hebrew University of Jerusalem","doi-asserted-by":"crossref","award":["UIUC-HUJI Joint seed grant"],"award-info":[{"award-number":["UIUC-HUJI Joint seed grant"]}],"id":[{"id":"10.13039\/501100003483","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2023,11,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Indiscriminate use of antibiotics has imposed a selective pressure for the rapid rise in bacterial resistance, creating an urgent need for novel therapeutics for managing bacterial infectious diseases while counteracting bacterial resistance. Carbapenem-resistant\n            <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n            strains have become a major challenge in modern medicine due to their ability to cause an array of severe infections. Recently, we have shown that the 20-mer random peptide mixtures are effective therapeutics against three ESKAPEE pathogens. Here, we evaluated the toxicity, biodistribution, bioavailability, and efficacy of the ultra-short palmitoylated 5-mer phenylalanine:lysine (FK5P) random peptide mixtures against multiple clinical isolates of carbapenem-resistant\n            <jats:italic>K. pneumoniae<\/jats:italic>\n            and\n            <jats:italic>K. oxytoca<\/jats:italic>\n            . We demonstrate the FK5P rapidly and effectively killed various strains of\n            <jats:italic>K. pneumoniae<\/jats:italic>\n            , inhibited the formation of biofilms, and disrupted mature biofilms. FK5P displayed strong toxicity profiles both\n            <jats:italic>in vitro<\/jats:italic>\n            and in mice, with prolonged favorable biodistribution and a long half-life. Significantly, FK5P reduced the bacterial burden in mouse models of acute pneumonia and bacteremia and increased the survival rate in a mouse model of bacteremia. Our results demonstrate that FK5P is a safe and promising therapy against\n            <jats:italic>Klebsiella<\/jats:italic>\n            species as well as other ESKAPEE pathogens.\n          <\/jats:p>","DOI":"10.1128\/aac.00574-23","type":"journal-article","created":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T13:01:58Z","timestamp":1697029318000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":7,"title":["Antibacterial efficacy of an ultra-short palmitoylated random peptide mixture in mouse models of infection by carbapenem-resistant\n            <i>Klebsiella pneumoniae<\/i>"],"prefix":"10.1128","volume":"67","author":[{"given":"Jonathan Z.","family":"Lau","sequence":"first","affiliation":[{"name":"Department of Pathobiology, University of Illinois at Urbana-Champaign , Urbana, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanny Hsuan","family":"Kuo","sequence":"additional","affiliation":[{"name":"Department of Pathobiology, University of Illinois at Urbana-Champaign , Urbana, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yael","family":"Belo","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry, Food Science and Nutrition, The Robert H. 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Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem , Rehovot, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hannah E.","family":"Caraway","sequence":"additional","affiliation":[{"name":"Department of Pathobiology, University of Illinois at Urbana-Champaign , Urbana, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Myung Whan","family":"Oh","sequence":"additional","affiliation":[{"name":"Department of Pathobiology, University of Illinois at Urbana-Champaign , Urbana, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Materials Science and Engineering, University of Illinois at Urbana-Champaign , Urbana, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nahed","family":"Ismail","sequence":"additional","affiliation":[{"name":"Department of Pathology, College of Medicine, University of Illinois at Chicago , Chicago, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7962-3950","authenticated-orcid":true,"given":"Gee W.","family":"Lau","sequence":"additional","affiliation":[{"name":"Department of Pathobiology, University of Illinois at Urbana-Champaign , Urbana, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3582-4029","authenticated-orcid":true,"given":"Zvi","family":"Hayouka","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry, Food Science and Nutrition, The Robert H. 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The antimicrobial peptides and their potential clinical applications. 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